Medical Radiology and Radiation Safety. 2015. Vol. 60. No. 6. P. 15-19

RADIATION MEDICINE

T.A. Astrelina¹, A.Yu. Bushmanov¹, I.V. Kobzeva¹, A.V. Akleyev^2,3, G.P. Dimov², P.S. Eremin¹, A.S. Samoilov¹

Evaluation of Skin Fibroblasts Proliferation and Secretion from Patients with Chronic Radiation Exposure

1. A.I. Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Urals Research Center for Radiation Medicine, Chelyabinsk, Russia; 3. Chelyabinsk State University, Russia

ABSTRACT

Purpose: To investigate skin fibroblasts proliferation and activity in patients with chronic radiation exposure.

Material and methods: The study of skin fibroblasts bioptates from patients with chronic radiation exposure (19 samples, the mean age was 66.1 ± 7.6 years) and healthy donors (15 samples). Culturing of skin fibroblasts were performed by standard methods. The average dose accumulated during chronic radiation exposure of the soft tissue was 0.03 ± 0.03 Gy in the study group, and 0.53 ± 0.75 Gy of the bone marrow. Study of proliferative activity of skin fibroblasts was carried out by Delta Cell Index for the normalized time point with the slope of the curve in the exponential growth phase using cell analyzer xCELLigence. Investigation of the secretory activity of skin fibroblasts (concentration vascular endothelial growth factor (VEGF), interleukin 6 (IL-6), brain-derived neurotrophic factor (BDNF), fibroblast growth factor (FGF) in the cultural medium were determined by ELISA kits (R & D Systems, USA).

Results: It is found that the normalized cell index delta of skin fibroblasts was higher in the skin fibroblasts of patients compared with the healthy donors (189.91 ± 21,35 EU and 108.67 ± 4.25 EU, respectively, p = 0.04), that is, the decrease of proliferative activity in the patients compared to the control group. The secretory activity of skin fibroblasts is different: the concentration level of VEGF and BDNF was higher in the healthy donors compared with patients -174.22 ± 12.21 and 86.37 ± 6.81 pg/ml, p = 0.04; 80.10 ± 7.22 and 12.66 ± 2.35 pg/ml, p = 0.03, respectively. The concentration of IL-6 in the culture medium of the healthy donors is lower than in the patients (26.45 ± 5.16 and 116.68 ± 10.01 pg/ml, p = 0.03). It was found that there is the correlation between the dose of chronic radiation exposure and the fibroblasts secretion activity and proliferative potential.

Conclusion: The dose accumulated during chronic radiation exposure affects the proliferation and activity of skin fibroblasts. The dose accumulated during chronic radiation exposure correlates with biochemical markers.

Key words: proliferation, secretion, skin fibroblasts, chronic radiation Exposure

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For citation: Astrelina TA, Bushmanov AYu, Kobzeva IV, Akleyev AV, Dimov GP, Eremin PS, Samoilov AS. Evaluation of Skin Fibroblasts Proliferation and Secretion from Patients with Chronic Radiation Exposure. Medical Radiology and Radiation Safety. 2015;60(6):15-9. Russian.

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