Medical Radiology and Radiation Safety. 2015. Vol. 60. No. 2. P. 5-8

RADIATION BIOLOGY

K.V. Kotenko, B.B. Moroz, Yu.B. Deshevoy, T.A. Nasonova, O.A. Dobrynina, V.G. Lebedev, A.V. Lirshikova, I.I. Eremin

Syngenic Multipotent Mesenchymal Stromal Cells in the Treatment of Persisted Radiation Skin Ulcers in the Experiment

A.I. Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

ABSTRACT

Purpose: The effectiveness of syngenic multipotent mesenchymal stromal cells (MMSC) in treatment of persisted radiation skin ulcers caused by relatively soft x-ray was studied.

Material and methods: Experiments were carried out on rats by the inbreed strain Wistar-Kyoto. Rats had been irradiated locally in lumbar-iliac region using x-ray machine RAP 100-110 with the dose of 110 Gy (30 kV tube voltage, current 6.1 ma, thick Al filter 0.1 mm). Dose rate is 173 Gy/min. 27 rats (from 40 being irradiated) with expanded radiation skin ulcers of the were selected on day 84 after exposure to radiation. This group of animals were divided into three groups (in accordance with the dimensions and clinical course of ulcers) Two groups of rats have been treated with transplantation of MMSC isolated from bone marrow and developed in vitro: once (in 85 days) or twice(on 85 and 93 days). MMSC (1.7×106 cells per injection) was injected under the skin around the radiation ulcer. The third group was the control.

Results: The double imposition of syngenic MMSC stimulated healing of persisted radiation ulcers, that became clear from 93 to 111 days after exposure.

Conclusion: The findings suggest that singenic MMSC can be effective in the treatment of persisted radiation skin ulcers.

Key words: multipotent mesenchymal stromal cells, X-ray, radiation skin ulcers

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For citation: Kotenko KV, Moroz BB, Deshevoy Yu.B, Nasonova TA, Dobrynina OA, Lebedev VG, Lirshikova AV, Eremin II. Syngenic Multipotent Mesenchymal Stromal Cells in the Treatment of Persisted Radiation Skin Ulcers in the Experiment. Medical Radiology and Radiation Safety. 2015;60(2):5-8. Russian.

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