Medical Radiology and Radiation Safety. 2015. Vol. 60. No. 1. P. 15-19

RADIATION BIOLOGY

I.I. Eremin¹, A.Yu. Bushmanov¹, A.V. Akleyev^2,3, G.P. Dimov², A.A. Pulin¹, I.N. Korsakov¹, P.S. Eremin¹, N.L. Lazareva¹, V.L. Zorin¹, K.V. Kotenko¹

The Influence of Ionizing Radiation on Fibroblasts’ Secretion and Proliferation

1. A.I. Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Urals Research Center for Radiation Medicine, Chelyabinsk region, Russia; 3. Chelyabinsk State University, Russia

ABSTRACT

Purpose: To evaluate the influence of the ionizing radiation on fibroblasts secretion activity and their proliferative potential.

Material and methods: Secretion activity and proliferative potential of fibroblasts gained from chronically exposed to ionizing radiation people (10 specimens) and healthy donors (10 specimens) were studied. Cell index, normalized cell index delta, and slope of the exponential growth curve were calculated, concentration of vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), brain-derived neurotrophic factor (BDNF), and fibroblast growth factor (FGF) in the cultural media were determined.

Results: Higher concentration of VEGF and BDNF in cultural fluid of fibroblasts gained from healthy volunteers was found. Concentration of IL-6 and cell doubling time were greater in patients exposed to ionizing radiation. Cell doubling time was bigger for fibroblasts gained from patients with lower dose of irradiation. It was found that there is the correlation between the dose of ionizing radiation and the fibroblasts secretion activity and proliferative potential.

Conclusion: Ionizing radiation affects fibroblasts secretion and proliferation on doze-dependent manner.

Key words: cell index, skin fibroblasts, chronic radiation exposure, experimental model

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For citation: Eremin II, Bushmanov AYu, Akleyev AV, Dimov GP, Pulin AA, Korsakov IN, Eremin PS, Lazareva NL, Zorin VL, Kotenko KV. FThe Influence of Ionizing Radiation on Fibroblasts’ Secretion and Proliferation. Medical Radiology and Radiation Safety. 2015;60(1):15-9. Russian.

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