Medical Radiology and Radiation Safety. 2023. Vol. 68. № 6

DOI:10.33266/1024-6177-2023-68-6-49-54

A.I. Kotikova, E.A. Blinova, A.V. Akleyev

Analysis of Vβ-Segment Diversity of T-Cell Receptor in Residents
of the Techa Riverside Villages Chronically Exposed to Radiation in the Long-Term Period

Urals Research Center for Radiation Medicine, Chelyabinsk, Russia

Chelyabinsk State University, Chelyabinsk, Russia

Contact person: Alisa Igorevna Kotikova, e-mail: Этот адрес электронной почты защищен от спам-ботов. У вас должен быть включен JavaScript для просмотра.

 

Abstract

Purpose: Study the repertoire of the T-cell receptor in persons chronically exposed to radiation in the long-term period.

Material and methods: The study involved 48 people, who were divided into two groups: a group of exposed persons –
31 individuals with the average accumulated dose to red bone marrow (RBM) of 981±130 mGy, and a comparison group –
17 individuals, the average accumulated dose to RBM was 25.3±5.91 mGy. The study groups did not differ significantly in age, gender and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index.

Results: The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (p=0.03 and p=0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor is uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups by the Gini-TCR index (p=0.14). 

Keywords: chronic radiation exposure, residents, the Techa river, peripheral blood, T-cell receptor, immunity

For citation: Kotikova AI, Blinova EA, Akleyev AV. Analysis of Vβ-Segment Diversity of T-Cell Receptor in Residents of the Techa Riverside Villages Chronically Exposed to Radiation in the Long-Term Period. Medical Radiology and Radiation Safety. 2023;68(6):49–54. (In Russian). DOI:10.33266/1024-6177-2023-68-6-49-54

 

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Conflict of interest. The authors declare no conflict of interest.

Financing.The work was carried out as part of the: “Study on the effect of chronic radiation exposure on the state of the human T-cell immune system”.

Contribution. Kotikova A.I. – methodology development, laboratory research, statistical processing, article authoring; Blinova E.A. – methodology development, article authoring; Akleyev A.V. – development of the research concept, scientific supervision, article authoring.

Article received: 20.07.2023. Accepted for publication: 27.08.2023.