Medical Radiology and Radiation Safety. 2023. Vol. 68. № 6
DOI:10.33266/1024-6177-2023-68-6-55-59
E.A. Kodintseva1, 2, A.А. Akleyev3
Effect of Radiation and Non-Radiation Factors on the ТBX21 Concentration in lysates of the Mitogen-Stimulated Mononuclear Cells
1 Urals Research Center for Radiation Medicine, Chelyabinsk, Russia
2 Chelyabinsk State University, Chelyabinsk, Russia
3 South-Ural State Medical University, Chelyabinsk, Russia
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ABSTRACT
Purpose: To evaluate the effect of radiation and non-radiation factors on the intracellular concentration of the ТBX21 transcription factor in peripheral blood PHA-stimulated mononuclear cells of chronically exposed residents of the Techa Riverside settlements in the long-term period after the start of exposure.
Material and methods: The main group consisted of 30 people aged 67–80 years with the mean dose to the red bone marrow 867±136 mGy, to thymus and peripheral lymphoid organs 125±20 mGy. The comparison group included 10 unexposed people aged 63–82 years. The main and the comparison groups had similar sex and ethnic composition. Peripheral blood mononuclear cells were PHA-stimulated for 24 hours. Cellular lysates were normalized by total protein concentration prior to being used for ELISA test.
Results: After 24 hours of incubation the median and interquartile range of the intracellular TBX21 concentration in mononuclear cells was 34.2 (6.6–86.0) pg/ml after the mitogen stimulation, and 0 (0–24.9) pg/ml – without mitogen stimulation (р=0.001). In the comparison group these values made up 24.8 (0.2–47.6) and 13.0 (0–19.2) pg/ml, respectively
Conclusion: The intracellular TBX21 concentration after 24-hour mitogen stimulation did not differ statistically significantly in chronically exposed and unexposed people, as well as in people from different dose groups. Statistically significant increase in TBX21 concentration in the lysates of mononuclear cells that were PHA-stimulated for 24 hours relative to non-mitogen stimulated cells was noted in chronically exposed people with medium and high doses to the red bone marrow. No correlation was revealed between the TBX21 concentration and dose characteristics, sex, and ethnicity of the studied individuals. The results are preliminary.
Keywords: chronic radiation exposure, residents, the Techa River, peripheral blood, ТBX21 transcription factor, lysates of mononuclear cells, Phytohemagglutinin
For citation: Kodintseva ЕА, Akleyev АА. Effect of Radiation and Non-Radiation Factors on the ТBX21 Concentration in lysates of the Mitogen-Stimulated Mononuclear Cells. Medical Radiology and Radiation Safety. 2023;68(6):55–59. (In Russian). DOI:10.33266/1024-6177-2023-68-6-55-59
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Conflict of interest. The authors declare no conflict of interest.
Financing. This study was carried out within the framework of the state assignment of the FMBA of Russia: “The state of human cellular immunity in the period of the development of the late effects of chronic radiation exposure”.
Contribution. All authors confirm that their authorship meets the international ICMJE criteria. Kodintseva Е.А. — participated in the development of the concept and design of the study, research, data analysis and interpretation, preparation of the article; read and approved the final version before publication. Akleуev А.А. — participated in the development of the concept and design of the study, interpretation of results, scientific editing; read and approved the final version before publication
Article received: 20.07.2023. Accepted for publication: 27.08.2023.