Medical Radiology and Radiation Safety. 2024. Vol. 69. № 3
DOI:10.33266/1024-6177-2024-69-3-19-25
T.R. Gaynutdinov1, 2, S.A. Ryzhkin1, 2, 3, 4, 5, K.N. Vagin1, 2, E.Yu. Trizna2,
S.E. Ohrimenko3, 6
Study of Clinical, Hematologic and Immunologic Parameters in Assessing the Anti-Radiation Efficacy of the Therapeutic Agent Based on the Microorganism Fusobacterium Necrophorum
1 Federal Center for Toxicological, Radiation and Biological Safety, Kazan, Russia
2 Kazan Federal University, Kazan, Russia
3 Russian Medical Academy of Continuing Professional Education, Moscow, Russia
4 Kazan State Medical University, Kazan
5 Academy of Sciences of the Republic of Tatarstan, Kazan, Russia
6 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Timur Rafkatovich Gaynutdinov, e-mail: Этот адрес электронной почты защищен от спам-ботов. У вас должен быть включен JavaScript для просмотра.
ABSTRACT
Purpose: To study clinical, hematologic and immunologic parameters in assessing the anti-radiation efficacy of the therapeutic agent based on the microorganism Fusobacterium necrophorum.
Material and methods: The studies on determination of the anti-radiation efficacy of the strains of microorganisms killed by gamma-irradiation were carried out on sexually mature sexless white mice and white rats with live weight of 18–20 and 180–200 g, respectively, divided into experimental and control groups according to the principle of analogs. Modeling of acute radiation sickness was carried out on a Puma gamma unit with a radioactive source of cesium-137 at a dose of LD100/30. As potential anti-radiation drugs we used inactivated by irradiation on the gamma unit Explorer preparations of microbial origin F. necrophorum strain 8. necrophorum strain 8TS630501 at doses of 15, 20, 25 and 30 kGy. The tested preparations were injected subcutaneously in the volume of 0.2 cm3 to white mice and 2.0 cm3 to white rats 3 days after radiation exposure.
Results: It was experimentally established that complete sterilization of the microbe occurs at doses of 25 and 30 kGy. The culture of
F. necrophorum, irradiated at doses of 25 and 30 kGy and administered to animals 3 days after external radiation exposure, promoted survival, preservation of 60 to 80 % of lethally irradiated white mice and rats. At the same time the recovery of leukocytes and hemoglobin number was slow and continued until the end of the study. In animals treated with the developed therapeutic agents, there was also a decrease in the number of T-cells, but it was less pronounced than in the irradiation control group. The number of B-lymphocytes was affected similarly to T-lymphocytes. The minimum of the number of B-lymphocytes in the experimental groups was noted at 14 days. Studies on the intensity of the process of lipid peroxidation (LPO) in the content of malonic dialdehyde in peripheral blood of gamma-irradiated, treated and intact rats, it was found that in the irradiated control group there is a significant increase in the LPO index in blood in relation to biological control and treatment groups.
Conclusion: It has been established that the highest anti-radiation efficacy is possessed by a therapeutic agent of microbial origin (RNF-30), which was obtained by gamma-irradiation at a dose of 30 kGy of the culture of F. necrophorum.
Keywords: acute radiation disease, Fusobacterium necrophorum, anti-radiation agent, laboratory animals, clinical, hematological and immunological parameters, radiotoxins, survival rate
For citation: Gaynutdinov TR, Ryzhkin SA, Vagin KN, Trizna EYu, Ohrimenko SE. Study of Clinical, Hematologic and Immunologic Parameters in Assessing the Anti-Radiation Efficacy of the Therapeutic Agent Based on the Microorganism Fusobacterium Necrophorum. Medical Radiology and Radiation Safety. 2024;69(3):19–25. (In Russian). DOI:10.33266/1024-6177-2024-69-3-19-25
References
1. Baird E., Reid C., Cancio L.C., Gurney J.M, Burmeister D.M. A Case Study Demonstrating Tolerance of the Gut to Large Volumes of Enteral Fluids in Burn Shock. Int J Burns Trauma. 2021;11(3):202–206. doi: 10.1002/14651858.CD007715.pub2. PMID: 34336386 PMCID: PMC8310868.
2. Cannon G., Kiang J.G. An Overview of the Impact of Radiation on Ecology: Wildlife Population. Int J Radiat Biol. 2020;1–9. Online ahead of print. Doi: 10.1080/09553002.2020.1793021. PMID: 32663058.
3. Burmeister D.M., Johnson T.R., Lai Z., Scroggins S., DeRosa M., Jonas R.B., Zhu C., Scherer E., Stewart R.M., Schwacha M.G., Jenkins D.H., Eastridge B.J., Nicholson S.E. The Gut Microbiome Distinguishes Mortality in Trauma Patients Upon Admission to the Emergency Department. J Trauma Acute Care Surg. 2020;88(5):579–587. Doi:10.1097/TA.0000000000002612 PMID: 32039976 PMCID: PMC7905995
4. Jones C.B., Davis C.M., Sfanos K.S. The Potential Effects of Radiation on the Gut-Brain Axis. Radiat Res. 2020;193(3):209–222. Doi: 10.1667/RR15493.1. PMID: 31898468 Review.
5. Kalkeri R., Walters K., Pol W.V.D., McFarland B.C., Fisher N., Koide F., Morrow C.D., Singh V.K. Changes in the Gut Microbiome Community of Nonhuman Primate Following Radiation Injury. BMC Microbiome. 2021;21(1):93. Doi: 10.1186/s12866-021-02146-w PMID: 33781201.
6. Kiang J.G., Smith J.T., Cannon G., Anderson M.N., Ho C., Zhai M., Cui W., Xiao M. Ghrelin, a Novel Therapy, Corrects Cytokine and NF-kB-AKT-MAPK Network and Mitigates Intestinal Injury Induced by Combined Radiation and Skin-Wound Trauma. Cell Biosci. 2020;10:63. Doi: 10.1186/s13578-020-00425-z PMID: 32426105.
7. Гайнутдинов Т.Р., Вагин К.Н., Рыжкин С.А. Способ лечения радиационно-термических ожогов // Радиация и риск. Бюллетень национального радиационно-эпидемиологического регистра. 2023. Т.32, №1. С 108–117. [Gaynutdinov T.R., Vagin K.N., Ryzhkin S.A. Method of Treatment of Radiation-Thermal Burns. Radiatsiya i Risk = Radiation and risk. Bulletin of the National Radiation-Epidemiological Register. 2023;32(1):108–117 (In Russ.)]. DOI: 10.21870/0131-3878-2023-32-1-108-117
8. DiCarlo A.L., Bandremer A.C., Hollingsworth B. A., Kasim S., Laniyonu A., Todd N.F., Wang S.J., Wertheimer E.R., Rios, C.I. Cutaneous Radiation Injuries: Models, Assessment and Treatments. Radiation research. 2020;194(3):315–344. Doi: 10.1667/RADE-20-00120.1.
9. Körmöndi S., Terhes G., Pál Z., Varga E., Harmati M., Buzás K., Urbán E. Human Pasteurellosis Health Risk for Elderly Persons Living with Companion Animals. Emerging infectious diseases. 2019;25(2):229–235. Doi: 10.3201/eid2502.180641.
10. Peng Z., Wang X., Zhou R., Chen H., Wilson B.A., Wu B. Pasteurella Multocida: Genotypes and Genomics. Microbiology and Molecular Biology Reviews: MMBR. 2019;83(4):e00014-19. Doi: 10.1128/MMBR.00014-19
11. Kannangara D.W., Pandya D., Patel P. Pasteurella multocida Infections with Unusual Modes of Transmission from Animals to Humans: a Study of 79 Cases with 34 Nonbite Transmissions. Vector Borne Zoonotic Dis. 2020;Sep;20(9):637–651. Doi: 10.1089/vbz.2019.2558. Epub 2020 May 18. PMID: 32423307.
12. Shome R., Deka R.P., Sahay S., Grace D., Lindahl J.F. Seroprevalence of Hemorrhagic Septicemia in Dairy Cows IN Assam, India. Infection Ecology and Epidemiology. 2019;9(1):1604064. Doi: 10.1080/20008686.2019.1604064.
13. Davis C.M., Allen A.R., Bowles D.E. Consequences of Space Radiation on the Brain and Cardiovascular System. J Environ Sci Health C Toxicol Carcinog. 2021;39(2):180–218. Doi: 10.1080/26896583.2021.1891825 PMID: 33902387
14. Gorbunov N.V., Kiang J.G. Brain Damage and Patterns of Neurovascular Disorder after Ionizing Irradiation. Complications in Radiotherapy and Radiation Combined Injury. Radiat Res. 2021;196(1):1–16. Doi: 10.1667/RADE-20-00147.1. PMID: 33979447.
15. Wang Z., Wang Q., Wang X., Zhu L., Chen J., Zhang B., Chen Y., Yuan, Z. Gut Microbial Dysbiosis is Associated with Development and Progression of Radiation Enteritis during Pelvic Radiotherapy. Journal of Cellular and Molecular Medicine. 2019;23(5):3747–3756. Doi: 10.1111/jcmm.14289
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out at the expense of the funds of the subsidy allocated by the Federal State Budgetary Institution “FCTRB-VNIVI” for the performance of research work, state registration No. 01200202604.
Contribution. T.R. Gaynutdinov – a literary review on the topic of the article was conducted, the experimental part of the work was performed, the received material was processed, the text was edited, the manuscript was prepared. S.A. Ryzhkin – scientific guidance. K.N. Vagin – advisory assistance was provided on the implementation of research. E.Y. Trizna –the experimental part of the work was performed. S.E. Okhrimenko – advisory assistance was provided in the implementation of the experimental part of the work..
Article received: 20.01.2024. Accepted for publication: 27.02.2024.