Медицинская радиология и радиационная безопасность. 2025. Том 70. № 1
DOI:10.33266/1024-6177-2025-70-1-21-29
А.В. Гутнов1, О.В. Белов2, Г.С. Качмазов1, Т.Т. Магкоев1,
Н.Р. Попова 3, Н.Е. Пухаева 1, 2
ВЛИЯНИЕ ОБЛУЧЕНИЯ ТЯЖЕЛЫМИ ИОНАМИ
НА МЕТАБОЛИЗМ ТЕХНОЛОГИЧЕСКИ И БИОЛОГИЧЕСКИ ЗНАЧИМЫХ МИКРООРГАНИЗМОВ:
БИОТЕХНОЛОГИЧЕСКИЕ ПЕРСПЕКТИВЫ ПРИМЕНЕНИЯ
1 Северо-Осетинский государственный университет, Владикавказ
2 Обьединенный институт ядерных исследований, Дубна
3 Институт теоретической и экспериментальной биофизики РАН, Пущино
Контактное лицо: А.В. Гутнов, e-mail: Этот адрес электронной почты защищен от спам-ботов. У вас должен быть включен JavaScript для просмотра.
РЕФЕРАТ
Цель: Провести обзор литературных данных по применению технологии тяжелоионного лучевого мутагенеза для селекции различных микроорганизмов, таких как бактерии, мицелиальные грибы, дрожжи и микроводоросли для биотехнологического применения.
Материал и методы: Собраны данные за последние 15 лет по метаболическим эффектам у мутантов, полученных облучением тяжелыми ионами, биотехнологически значимых микробиологических объектов (бактерии, грибы, водоросли).
Результаты: Обсуждаются биотехнологическая значимость, а также генетические, морфологические и прочие аспекты обнаруженных изменений мутантных микробиологических объектов. В настоящее время мутагенез, индуцированный тяжелоионным облучением с высокой линейной передачей энергии и высокой биологической эффективностью, признан в качестве нового мощного метода для создания микробных штаммов с ранее не известными свойствами. Мы полагаем, что направленная селекция с помощью тяжелоионного мутагенеза внесет большой прогрессивный вклад в моделирование промышленных штаммов-продуцентов для биотехнологии.
Заключение: Исследования, описанные в данном обзоре, позволяют предположить, что применение ионно-лучевой мутагенной технологии для микроорганизмов полезно как для фундаментальной науки, так и для прикладных исследований.
Ключевые слова: биотехнология, микроорганизмы, метаболизм, мутагенез, облучение тяжелыми ионами
Для цитирования: Гутнов А.В., Белов О.В., Качмазов Г.С., Магкоев Т.Т., Попова Н.Р., Пухаева Н.Е. Влияние облучения тяжелыми ионами на метаболизм технологически и биологически значимых микроорганизмов: биотехнологические перспективы применения // Медицинская радиология и радиационная безопасность. 2025. Т. 70. № 1. С. 21–29. DOI:10.33266/1024-6177-2025-70-1-21-29
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Поступила: 20.10.2024. Принята к публикации: 25.11.2024.