Medical Radiology and Radiation Safety. 2024. Vol. 69. № 1
DOI:10.33266/1024-6177-2024-69-1-61-66
T.V. Vishnevskaya1, D.S. Isubakova1, M.Yu. Tsyplenkova1, O.S. Tsymbal1, I.V. Milto1, 2, R.M. Takhauov1, 2
Comparative Retrospective Analysis of the Results of Cytogenetic Studies of Employees of the Object of Use of Ionizing Radiation
1 Seversk Biophysical Research Center of the Federal Medical-Biological Agency, Seversk, Russia
2 Siberian State Medical University, Tomsk, Russia
Contact person: T.V. Vishnevskaya, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. , This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To conduct a comparative retrospective analysis of the results of cytogenetic studies of workers at facilities using ionizing radiation in 2003 and 2018.
Material and methods: The material for the study was the venous blood of workers at facilities using ionizing radiation. The study was conducted on conditionally healthy workers (n = 11), of which 2 groups were formed: the control group (blood sampling and cytogenetic study of blood lymphocytes were carried out in 2003) and the study group (blood sampling was carried out in 2018). For all examined individuals, blood lymphocytes were cultured and standard cytogenetic analysis followed by statistical processing of the results.
Results: A comparative retrospective analysis showed that in the study group (2018) compared to the control group (2003), the frequency of chromatid fragments was reduced (p = 0.0452). The frequencies of other types of cytogenetic disorders studied (aberrant cells, chromosomal fragments, dicentric and circular chromosomes) do not differ between groups.
Cytogenetic abnormalities in blood lymphocytes are a highly sensitive measure of the degree of radiation exposure in the early and late periods after exposure and can be used as a biological indicator of ionizing radiation.
The absence of differences in indicators of radiation exposure markers may be due to the large interval of time that elapsed after irradiation until the moment of examination (15 years), during which lymphocytes with chromosomal aberrations were eliminated from the blood. In the future, for the completeness of the retrospective analysis and the accuracy of the result, it is planned to conduct a study on a larger sample with a shorter time interval between cytogenetic studies.
Conclusion: The result of this work allows us to supplement the understanding of the mutation process in the somatic cells of individuals exposed to ionizing radiation in the course of professional activities and indicates genotoxicity.
Keywords: occupational exposure, cytogenetic study, retrospective analysis, chromosomal aberrations, blood lymphocytes
For citation: Vishnevskaya TV, Isubakova DS, Tsyplenkova MYu, Tsymbal OS, Milto IV, Takhauov RM.Comparative Retrospective Analysis of the Results of Cytogenetic Studies of Employees of the Object of Use of Ionizing Radiation. Medical Radiology and Radiation Safety. 2024;69(1):61–66. (In Russian). DOI:10.33266/1024-6177-2024-69-1-61-66
References
1. Akleyev A.V., Krestinina L.Yu., Varfolomeeva T.A., Ostroumova E.V., Pushkarev S.A., Shalaginov S.A., Khudyakova O.I., Veremeeva G.A., Vozilova A.V., Hall P. Medico-Biological Effects of Chronic Exposure to Ionizing Radiation on Humans. Medical Science and Education of the Urals. 2008;9;2:8–10 (In Russ.).
2. Ivanitskaya M.V., Isayeva L.N., Yachmenev V.A., Govorun A.P., Liksonov V.I., Potapov V.N., Chesnokov A.V., Shcherbak S.B. Distribution of 137Cs Pollution Levels in the Floodplain of the Techa River in the Village of Brodokalmak. Problems of Ecology of the Southern Urals. 1996;1:7–18 (In Russ.).
3. Akleyev A.V., Degteva M.O., Krestinina L.Yu. Radioepidemiological Studies in the Urals: Outcomes and Future Directions. Radiatsionnaya Gigiyena = Radiation Hygiene. 2021;14(4):31–44. DOI: 10.21514/1998-426X-2021-14-4-31-44 (In Russ.).
4. Neronova E.G., Aleksanin S.S. Evaluation of Cytogenetic Parameters in Persons Who Have Contacted with Ionizing Radiation. Mediko-Biologicheskiye i Sotsialno-Psikhologicheskiye Problemy Bezopasnosti v Chrezvychaynykh Situatsiyakh = Medicо-Biological and Socio-Psychological Problems of Safety in Emergency Situations. 2014;1:70–76 (In Russ.).
5. Petushkova V.V., Pelevina I.I., Serebryanyy A.M., Kogarko I.N., Kogarko B.S., Akleyev A.V., Azizova T.V., Neifakh E.A., Aleshchenko A.V., Ganeyev I.I., Ktitorova O.V. Some Approaches to the Analysis of the Structure of the Adaptive Response in Occupational Exposure. Radiatsiya i Risk = Radiation and Risk. 2020;29;4:97–105. DOI: 10.21870/0131-3878-2020-29-4-97-105 (In Russ.).
6. Volkov A.N., Nacheva L.V. Cytogenetic Methods in the Practice of Modern Biomedical Research. Part I: History and Theoretical Foundations of Human Cytogenetics. Fundamentalnaya i Klinicheskaya Meditsina = Fundamental and Clinical Medicine. 2021;6;4:142–150. DOI: 10.23946/2500-0764-2021-6-4-142-150 (In Russ.).
7. Aleksanin S.S., Slozina N.M., Neronova E.G. Emergencies and the Human Genome. Ministry of the Russian Federation for Civil Defense, Emergency Situations and Elimination of Consequences of Natural Disasters, All-Russian Center for Emergency and Radiation Medicine A.M. Nikiforova. St. Petersburg Publ., 2010. 84 p. (In Russ.).
8. Takhauov R.M., Karpov A.B., Zerenkov A.G., Ovchinnikov A.V., Izmestiev K.M., Spasibenko P.V., et al. Medical and Dosimetric Register of the Personnel of the Siberian Chemical Plant – a Basis for Assessing the Effects of Chronic Exposure. Radiatsionnaya Biologiya. Radioekologiya = Radiation Biology. Radioecology. 2015;55;5:467–473 (In Russ.).
9. Takhauov R.M., Karpov A.B., Albach E.N., Khalyuzova M.V., Freidin M.B., Litviakov N.V., Sazonov A.E., Isubakova D.S., Bolshakov M.A., Mezheritskiy S.A., Mironova E.B., Semenova J.V., Nekrasov G.B., Izosimov A.S., Gagarin A.A., Brendakov R.V., Maksimov D.E., Ermolaev Y.D. The Bank of Biological Samples Representing Individuals Exposed to Long-Term Ionizing Radiation at Various Doses. Biopreservation and Biobanking. 2015;13;2:72–78. DOI: 10.1089/bio.2014.0035.
10. Snigireva G.P. Biological Dosimetry Based on Cytogenetic Analysis. Bulletin of the Russian Scientific Center for Roentgen Radiology of the Ministry of Health of Russia. 2011;11;1:18 (In Russ.).
11. Khalyuzova M.V., Tsyganov M.M., Isubakova D.S., Bronikovskaya E.V., Usova T.V., Litvyakov N.V., Karpov A.B., Takhauova L.R., Takhauov R.M. Genome-Wide Associative Study of the Association of Polymorphic Loci with an Increased Frequency of Chromosomal Aberrations in Individuals Exposed to Prolonged Radiation Exposure. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2019;64;4:32–40. DOI: 10.12737/article_5d1103efefe893.65968050 (In Russ.).
12. Volkov A.N., Rytenkova O.I. Cytogenetic Methods in the Practice of Modern Biomedical Research. Part III: Numerical Anomalies of the Human Karyotype. Fundamentalnaya i Klinicheskaya Meditsina = Fundamental and Clinical Medicine. 2022;7;3:85–96. DOI: 10.23946/2500-0764-2022-7-3-85-96 (In Russ.).
13. Apsalikov K.N., Muldagaliev T.Zh., Belikhina T.I., Tanatova Z.A., Kenzhina L.B. Analysis and Retrospective Evaluation of the Results of Cytogenetic Examinations of the Population of Kazakhstan Exposed to Radiation as a Result of Nuclear Weapons Testing at the Semipalatinsk Test Site, and Their Descendants. Medico-Biological Problems of Life. 2013:9;1:42–49 (In Russ.).
14. Cherednichenko O.G. Stable Aberrations of Chromosomes During Long-Term Cultivation of Lymphocytes and in the Process of Formation of a Radioadaptive Response. Bulletin of KazNU. Biological Series. 2011;1;47:49–53 (In Russ.).
15. Neronova E., Slozina N., Nikiforov A. Chromosome Alterations in Cleanup Workers Sampled Years after the Chernobyl Accident. Radiat. Res. 2003;160;1:46–51. DOI: 10.1667/0033-7587(2003)160[0046:CAICWS]2.0.CO;2.
16. Yarmonenko S.P., Vaynson A.A. Radiobiology of Man and Animals. Tutorial. Moscow, Higher school Publ., 2004. 549 p. (In Russ.).
17. Isubakova D.S., Litvyakov N.V., Tsymbal O.S., Usova T.V., Tsyplenkova M.Yu., Milto I.V., Takhauov R.M. Search for Polymorphic Variants of Candidate genes of Individual Radiosensitivity. Bulletin of Siberian Medicine. 2022;21;4:79–87. DOI: 10.20538/1682-0363-2022-4-79-87 (In Russ.).
18. Vozilova A.V., Akhmadullina Yu.R. The Study of Individual Radiosensitivity in Humans Based on the Assessment of the Frequency of Chromosomal Aberrations and Micronuclei in T-lymphocytes of Peripheral Blood. Genetika = Russian Journal of Genetics. 2019;55;10:1180–1188 (In Russ.).
19. Ivanova T.V. Cytogenetic Research Methods: the Possibilities of Laboratory Diagnostics and Information Content for the Doctor. Therapist. 2020; 5:12–19 (In Russ.).
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Conflict of interest. The authors declare no conflict of interest.
Financing. The research was carried out within the framework of the state task, the research topic is «Studying the relationship of single-nucleotide polymorphisms of cell cycle genes with the frequency of chromosomal aberrations in blood lymphocytes of employees of the Siberian Chemical Plant».
Contribution. Vishnevskaya T.V. – preparation of the text of the article, analysis and interpretation of data, conducting experiments, collection and analysis of literary material, development of the concept and design of the study; Isubakova D.S. – development of the concept and design of the study, internal audit; Tsyplenkova M.Yu., Tsymbal O.S. – conducting experiments and statistical data processing; Milto I.V. – scientific editing of the text, verification of critically important intellectual content; Takhauov R.M. – development of the concept and design of the study, approval of the final version of the manuscript.
Article received: 20.10.2023. Accepted for publication: 27.11.2023.