JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2021. Vol. 66. № 4. P. 5–12
Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions
A.A. Rastorgueva, T.A. Astrelina, V.A. Brunchukov, D.Yu. Usupzhanova, I.V. Kobzeva, V.A. Nikitina,
S.V. Lischuk, E.A. Dubova, K.A. Pavlov, V.A. Brumberg, A.S. Samoilov
A.I .Burnasyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Tatiana Alexandrovna Astrelina: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Background: To compare the results of the use of mesenchymal stromal cells (MSCs) of human gingival mucosa and MSCs of rat gingival mucosa, their conditioned media, and to evaluate their effect on tissue regeneration in local radiation injury (LRI).
Material and methods: The study included 120 white male Wistar rats weighing 210 ± 30 g at the age of 8–12 weeks, randomized into 6 groups (20 animals each): control (C), animals did not receive therapy; control with the introduction of culture medium concentrate (CM) three times for 1, 14, 21 days; administration of human gingival mucosa MSCs (HM) at a dose of 2 million per 1 kg three times for 1, 14, 21 days; administration of human gingival mucosa MSCS conditioned medium concentrate (HMCM) at a calculated dose of 2 million cells per 1 kg three times for 1, 14, 21 days; administration of rat gingival mucosal MSCs (RM) at a dose of 2 million cells per 1 kg three times for 1, 14, 21 days; administration of rat gingival mucosal MSCS (RMCM) conditioned medium concentrate at a calculated dose of 2 million cells per 1 kg three times for 1, 14, 21 days. Each laboratory animal was observed 17 times: on 1, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, 98, 105, 112 day after the burn simulation. Histological (hematoxylin-eosin staining) and immunohistochemical (CD31, CD68, VEGF, PGP 9.5, MMP2,9, Collag 1, TIMP 2) studies were performed. LRI was modeled on an X-ray machine at a dose of 110 Gy. MSCs were cultured according to the standard method up to 3–5 passages, the conditioned medium was taken and concentrated 10 times. The immunophenotype of MSCs (CD34, CD45, CD90, CD105, CD73, HLA-DR) and viability (7‑ADD) were determined by flow cytofluorimetry.
Results: In a comparative analysis with the control group (C), starting from the 42nd day of the study, a tendency to reduce the area of skin ulcers in animals in all groups was observed, despite the fact that not all days had statistically significant differences. On day 112th, complete healing of skin ulcers in the CM group was observed in 40 % of animals in the HM group – in 60 %, in the HMCM group – in 20 %
of animals, in the RMCM group–20 %, and in the C and RM groups there were no animals with a prolonged wound defect.
Positive expression of the VEGF marker was observed in groups C and CM on the 28th day and in experimental groups (HM, HMCM, RM, RMCM) on the 112th day. A statistically significant increase in the CD68 marker was observed in groups C, RM, and RMCM, while the remaining groups showed a decrease in the number of macrophages.
Conclusion: Thus, all the treatment methods used, including the administration of MSCs, culture concentrates and conditioned media at a dose of 2 million per 1 kg, were effective in treating skin LRI and led to a reduction in the lesion area, accelerated ulcer healing, and improved regenerative processes. In addition, the use of mesenchymal stromal cells of the human gum mucosa led to an improvement in vascularization and a decrease in inflammatory processes in the focus of radiation damage to a greater extent than similar cells obtained from a rat.
Key words: mesenchymal stromal cells, local radiation lesions, conditioned medium, cell technologies, X-ray radiation, skin
For citation: Rastorgueva AA, Astrelina TA, Brunchukov VA, Usupzhanova DYu, Kobzeva IV, Nikitina VA, Lischuk SV, Dubova EA, Pavlov KA, Brumberg VA, Samoilov AS. Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions. Medical Radiology and Radiation Safety. 2021;66(4):5–12.
DOI: 10.12737/1024-6177-2021-66-4-5-12
References
1. Brunchukov VA, Astrelina TA, Nikitina VA, Kobzeva IV, Suchkova YuB, Usupzhanova DYu, et. al. The use of Placental Mesenchymal Stromal Cells in Local Radiation Skin Lesions. Genes and Cells. 2019, V. 14, Appendix 41p. (In Russian).
2. Eremin P. S., Deev R. V., Bozo I. Ya., Deshoy Yu. B., Lebedev V. G., Eremin I. I., et al. Healing of Tissues in the Area of Severe Local Radiation Damage to the Skin During Gene-mediated Induction of Angiogenesis with Neovasculgen. Journal of Anatomy and Histopathology. 2020; 9(2) 26–34 DOI: 10.18499/2225-7357-2020-9-2-26-34 (In Russian).
3. Kotenko KV, Eremin II, Moroz BB, Bushmanov AYu, Nadezhina NM, Galstyan IA, Grinakovskaya OS, Aksinenko AV, Deshoy YuB, Lebedev VG,. Svobodina TS, Zhgutov YuA, Lauk-Dubitsky SE, Eremin PS. Cell Technologies in the Treatment of Radiation Burns: the Experience of the Burnazyan Federal Medical Center. Cell Transplantology and Tissue Engineering. 2012, 7(2) P. 97–102 (In Russian).
4. Zheng K, Wu W, Yang S, Huang L, Chen J, Gong C et al. Bone Marrow Mesenchymal Stem Cell Implantation for the Treatment of Radioactivity Induced Acute Skin Damage in Rats. Mol Med Rep. 2015. 12(5). P. 7065–7071.
5. Da Silva Meirelles L, Caplan A, Nardi N. In Search of the In Vivo Identity of Mesenchymal Stem Cells. Stem Cells. 2008. 26(9) 2287–2299. DOI:10.1634/Stemcells. 2007-1122.
6. Rastorgueva A A, Astrelina TA, Brunchukov VA, et al. Evaluation of the Therapeutic Potential of the Conditioned Media of Mesenchymal Stem Cells in Chemical Burns in Laboratory Animals. Genes and Cells. 2019. 14. Appendix: 192–193 (In Russian).
7. Temnov AA, Astrelina TA, Rogov KA, et al. To Study the Influence of Factors of Conditioned Environment Obtained During the Cultivation of Bone Marrow Mesenchymal Stromal Cells on the Course of Severe Local Radiation Skin Lesions in Rats. Medical Radiology and Radiation safety. 2018;63(1):35–39. (In Russian).
8. Brunchukov VA, Astrelina TA, Nikitina VA, Kobzeva IV, Suchkova YuB, Usupzhanova DYu, Rastorgueva AA, Karaseva TV, Gordeev TV, Maxsimova OA, Naumova LA, Lischuk SV, Dubova EA, Pavlov KA, Brumberg VA, Makhova AE, Lomonosova EE, Dobrovolsskaya EI, Barabash IM, Bushmanov AYu, Samoilov AS. Experimental Treatment of Radiation Skin Lesions with Mesenchymal Stem Cells and Their Conditioned Media. Medical Radiology and Radiation Safety. 2020;65(1):5-12. DOI: 10.12737/1024-6177-2020-65-1-5-12 (In Russian).
9. Xu S, Liu C, Ji H. Concise Review: Therapeutic Potential of the Mesenchymal Stem Cell Derived Secretome and Extracellular Vesicles for Radiation-Induced Lung Injury: Progress and Hypotheses. Stem Cells Transl Med. 2019; 8(4):344–354. DOI:10.1002/sctm.18-0038.
10. Zuo R, Liu M, Wang Y et al. Correction to: BM-MSC-Derived Exosomes Alleviate Radiation-Induced Bone Loss by Restoring the Function of Recipient BM-MSCs and Activating Wnt/β-catenin Signaling. Stem Cell Res Ther. 2020; 11(1) DOI:10.1186/s13287-020-1553-x.
11. Scuteri A, Donzelli E, Foudah D et al. Mesengenic Differentiation: Comparison of Human and Rat Bone Marrow Mesenchymal Stem Cells. Int J Stem Cells. 2014; 7(2):127–134. DOI:10.15283/ijsc. 2014.7.2.127.
12. Orbay H, Devi K, Williams P, Dehghani T, Silva E, Sahar D. Comparison of Endothelial Differentiation Capacities of Human and Rat Adipose-Derived Stem Cells. Plast Reconstr Surg. 2016; 138(6):1231–1241. DOI:10.1097/prs.0000000000002791.
13. Iacovelli N, Torrente Y, Ciuffreda A et al. Topical Treatment of Radiation-Induced Dermatitis: Current Issues and Potential Solutions. Drugs Context. 2020; 9 : 1-13. DOI:10.7573/dic.2020-4-7
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 16.03.2021.
Accepted for publication: 21.04.2021.