JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2017. Vol. 62. No. 1. P. 5-11
DOI: 10.12737/25028
Transplantation of Autologous Cells of Stromal Vascular Fraction of Adipose Tissue in Severe Local Radiation Injuries of Skin Caused by X-rays
V.G. Lebedev, T.A. Nasonova, Yu.B. Deshevoy, A.V. Lyrschikova, O.A. Dobrynina,
A.S. Samoylov, A.Yu. Bushmanov, B.B. Moroz
A.I. Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
V.G. Lebedev – leading researcher, PhD in Biological Sciences; T.A. Nasonova – leading researcher, PhD in Medical Sciences; Yu.B. Deshevoy – leading researcher, PhD in Medical Sciences; A.V. Lyrschikova – leading researcher, PhD in Biological Sciences; O.A. Dobrynina – junior research fellow; A.S. Samoylov – General Director SRC-FMBC, MD; A.Yu. Bushmanov – First Deputy General Director, MD, Professor; B.B. Moroz – Head of Lab., Academician of RAS, MD.
Abstract
Purpose: To investigate the effectiveness of autologous cells of stromal vascular fraction of adipose tissue in severe local radiation skin injuries after the exposure of rats to X-rays.
Material and methods: Experiments were performed on Wistar rats, weighing 200–230 g. Rats were exposed locally in iliolumbar region using X-ray machine LNC-268 (RAP 100-10) at a dose of 110 Gy (30 kV tube voltage, current 6.1 mA, thick Al filter 0.1 mm), dose rate: 17.34 Gy/min. Area of the irradiation field was 8.2–8.5 cm2. Transplantation of autologous cells of stromal vascular fraction (SVFC) of adipose tissue was carried out on 21st or 35th days after irradiation. SVFC isolation was performed by means of enzymatic treatment of adipose tissue. SVFC suspension was administered subcutaneously at a dose of 1×106 cells per injection around the radiation ulcers.The severity of radiation damage to the skin and the effects of cellular therapy were evaluated in the dynamics of clinical manifestations, with the help of plane geometry and pathomorphometry.
Results: It was found that by the 17–25th day after irradiation radiation ulcers were formedon rat skin. In the control group of animalsulcers persisted throughout the observation period of more than 3 months. The area of ulcers was 1,87 ± 0,35 cm2 and 1.52 ± 0.24 cm2 at 83th and 90th days after irradiation, respectively. In animals of the experimental group, with autologous stromal vascular fraction of adipose tissue, was significant decrease in ulceration the area in comparison to control animals. In 80 % of the rats treated with SVFC on 21st day after exposure, to the 90th day after irradiation complete healing of ulcers occurred with the formation of atrophic scar at the site of radiation injuries. These clinical observations and planimetric were correlated with the results of histomorphometry.
Conclusion: Transplantation autologous SVFC of adipose tissue contributes to accelerate the healing of radiation ulcers after local x-ray exposure in the experiment, indicating that the prospects of using adipose tissue cell products for the treatment of severe local radiation injuries.
Key words: stromal vascular fraction, adipose tissue, cell technology, local radiation damage, multipotent mesenchymal stromal cells, radiation skin ulcers
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For citation: Lebedev VG, Nasonova TA, Deshevoy YuB, Lyrschikova AV, Dobrynina OA, Samoylov AS, Bushmanov AYu, Moroz B.B. Transplantation of Autologous Cells of Stromal Vascular Fraction of Adipose Tissue in Severe Local Radiation Injuries of Skin Caused by X-rays. Medical Radiology and Radiation Safety. 2017;62(1):5-11. Russian. DOI: 10.12737/25028
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