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The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2022. Vol. 67. № 3
E.V. Golub, V.V. Polkin, G.F. Mikhailova,
T.G. Shkavrova, V.V. Tsepenko, F.E. Sevrukov, V.S. Medvedev
Detection of Radiosensivity of Patients with Oral Cancer Using the Frequency of Cells
with Polysomy 7 and 11 Chromosomes in Process of Fractionated Gamma-Radiotherapy
A.F. Tsyb Medical Radiological Research Center, Obninsk, Russia
Contact person: E.V. Golub, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To study the dynamics of frequency of tumor cells with polysomy 7 and 11 chromosomes in patients with oral cancer during fractionated gamma-radiotherapy.
Material and methods: The study was carried out using fluorescence in situ hybridization (FISH) on smears of 19 patients with oral cancer before treatment, after first total focal dose of gamma-radiotherapy (up to 18 Gy) and after second total focal dose (up to 32 Gy). As for control oral smears of 12 healthy donors were taken. Molecular cytogenetic abnormalities were examined in taken samples.
Results: The average group frequency of cell with polysomy 7 and 11 chromosomes in controls was 0.7 ± 0.2 % and 0.3 ± 0.1 % respectively. The average frequency of cells with chromosome 7 polysomy in group oral cancer patients before treatment was 32.3 ± 4.5 %, after first focal dose was 15.7 ± 2.9 % and after second focal dose was 8.0 ± 2.3 %. Dynamics analysis of these values revealed the significant decreasing (р < 0.05) in cells with chromosome 7 polysomy during radiotherapy. The average group frequency of cells with polysomy
11 in cancer patients before treatment was 25.8 ± 5.6 %, 12.2 ± 2.4 % and 5.4 ± 0.9 % after first and second focal dose respectively. These values was significantly (р < 0.01) higher compare with the one in the control group. After radiotherapy the 16 (84 %) patients with polysomy chromosomes 7 and/or 11 had significant decreasing (р < 0.05) in aberrant levels. Some of them (6 persons – 32 %) had decreasing frequency up to the control levels. 4 persons (21 %) observed firstly the increasing of cells with polysomy but after there was the reduction. 5 patients (26 %) over full course of gamma-radiotherapy demonstrated the gain in frequency of cells with polysomy or the same value as it was before treatment.
Conclusion: Thus, the study of molecular cytogenetic abnormalities in cells of oral cancer patients before and after radiotherapy was shown the polysomy of chromosomes being the marker of genome instability could indicate the tumor response ongoing therapy.
Keywords: oral cancer, polysomy chromosomes, FISH, radiosensitivity, fractionated radiotherapy
For citation: Golub EV, Polkin VV, Mikhailova GF, Shkavrova TG, Tsepenko VV, Sevrukov FE, Medvedev VS. Detection of Radiosensivity of Patients with Oral Cancer Using the Frequency of Cells with Polysomy 7 and 11 Chromosomes in Process of Fractionated Gamma-Radiotherapy. Medical Radiology and Radiation Safety. 2022;67(3):47–52. (In Russian). DOI:10.33266/1024-6177-2022-67-3-47-52
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 17.01.2022. Accepted for publication: 15.03.2022.