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The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2022. Vol. 67. № 4
Three-Color FISH Method: Translocations in Peripheral Blood Lymphocytes Cultures before and after Local Gamma Radiation for Breast Cancer
V.Yu. Nugis, T.A. Astrelina, V.A. Nikitina, M.G. Kozlova,
I.V. Kobzeva, E.E. Lomonosova, Yu.B. Suchkova,
D.Yu. Usupzhanova, V.A. Brunchukov, A.A. Rastorgueva,
T.F. Malivanova, V.A. Brumberg,T.V. Karaseva, E.S. Lyubaeva,
D.Yu. Bobrov, N.G. Stepanyants, M.Yu. Sukhova, A.S. Samoilov
A.I. Burnasyan Federal Medical Biophysical Center of FMBA of Russia, Moscow, Russia
Контактное лицо: Nugis V.Yu., E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Study of chromosomal aberration yields with a three-color FISH method in the peripheral blood lymphocyte cultures of patients with breast cancer before and after local irradiation.
Material and methods: Venous blood was obtained from 24 patients aged from 33 to 79 years which suffering from cancer of the left or right breast after the implementation of a sectoral or radical resection of an affected organ with subsequent local adjuvant distant radiation therapy (DRT) to the scar area. Also it could be preceded by adjuvant polychimotherapy (PCT). A linear accelerator of the Trilogy system based on the Clinac iX platform was used for local irradiation. SOD was installed at 50 Gy per 25 fractions (daily 2 Gy). venous blood sampling produced to PCT and/or DRT and after 1 and/or 3 months after irradiation. A set of DNA probes to 1, 4 and 12 pairs of chromosomes was used for three-color FISH staining.
Results: Levels of translocations, dicentrics and acentrics (per 100 cells) in peripheral blood lymphocyte cultures of patients up to DRT did not differ significantly with and without PCT and were amounted to average 0.63 ± 0.10; 0.10 ± 0.04 and 0.17 ± 0.06, respectively. The frequency of these chromosome rearrangements significantly increased after radiation therapy by an average of up to 4.62 ± 0.46; 1.48 ± 0.19 and 0.95 ± 0.14. The differences between 1 and 3 months of taking material after irradiation were absent. One of the patients, unlike the rest, was cytogenetically examined 11 years after a similar local irradiation, and the frequencies of translocations and dicentrics in her lymphocyte culture were 4.56 and 0.19 (per 100 cells), respectively, while they were varied from 1.63 to 10.00 for translocations and from 0.27 (0 in one patient) to 4.06 for dicentrics for other patients.
Conclusion: When using a three-color FISH method there was a significant increase in the frequencies of translocations, dicentrics and acentrics in peripheral blood lymphocyte cultures of patients after DRT of breast cancer. At the same time there were more translocations than dicentrics and, apparently, they remain at the initial level for a long time. The frequency of translocations per 1 cell with translocations in the peripheral blood lymphocyte cultures in most cases exceeds 1 after local DRT of the breast cancer. The correlation between average physical doses to the whole body and cytogenetical estimates in individual patients was statistically insignificant.
Keywords: peripheral blood lymphocytes culture, three-color FISH method, translocations, breast cancer, local gamma-irradiation
For citation: Nugis VYu, Astrelina TA, Nikitina VA, Kozlova MG, Kobzeva IV, Lomonosova EE, Suchkova YuB, Usupzhanova DYu, Brunchukov VA, Rastorgueva AA, Malivanova TF, Brumberg VA, Karaseva TV, Lyubaeva ES, Bobrov DYu, Stepanyants NG, Sukhova MYu, Samoilov AS. Three-Color FISH Method: Translocations in Peripheral Blood Lymphocytes Cultures before and after Local Gamma Radiation for Breast Cancer. Medical Radiology and Radiation Safety. 2022;67(4):62-68. DOI: 10.33266/1024-6177-2022-67-4-62-68
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Informed Consent
All patients signed an informed consent to participate in the study.
Article received: 11.04.2022. Accepted for publication: 11.05.2022