JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2022. Vol. 67. № 6
DOI: 10.33266/1024-6177-2022-67-6-5-11
I.V. Kobzeva1, T.A. Astrelina1, V.A. Brunchukov1, V.A. Brumberg1, A.A. Rastorgueva1,
Yu.B. Suchkova1, D.Yu. Usupzhanova1, T.F. Malivanova1, V.A. Nikitina1, S.V. Lishchuk1,
E.A. Dubova1, K.A. Pavlov1, Ya.V. Tonkal1, O.F. Serova1,2, A.S. Samoilov1
Transplantation of Human Decellularized Amniotic Membrane in Local Radiation Injuries
1A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2Moscow Regional Perinatal Center, Balashikha, Russia
Contact person: T.A. Astrelina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Evaluation of the effectiveness of the use of human decellularized amniotic membrane, both as an independent covering material and as a cell-free matrix for syngeneic regenerative cells of adipose tissue (stromal-vascular fraction – SVF, adipose tissue ‒ AT) in local radiation injuries (LRI) IIIb-IV severity in laboratory animals.
Material and methods: 42 laboratory animals were studied. LRI modeling was carried out on an X-ray at a dose of 110 Gy. Animals were randomized randomly and divided into 6 groups depending on the type of treatment:
1st group (K) ‒ control group animals after irradiation did not receive specific treatment; the 2nd group (Gl) ‒ after irradiation, medical glue BF-6 was applied to the ulcer surface on the 21st day; 3rd group (Am) ‒ animals after irradiation on the ulcer surface was applied decellularized amniotic membrane, fixed with interrupted sutures on the 21st day; 4th group (Am-Gl) ‒ animals after irradiation on the ulcer surface was applied decellularized amniotic membrane, fixed with medical adhesive BF-6 for 21 days; 5th group (SVF-Gl) ‒ after irradiation, the SVF AT at a dose of 0.4×106 cells was applied to the ulcer surface after irradiation, followed by fixation with BF-6 medical glue for
21 days; the 6th group (Am-SVF) ‒ after irradiation, SVF AT was applied to the ulcer surface at a dose of 0.4×106 cells under the decellularized amniotic membrane, fixed with interrupted sutures on the 21st day.
Results: On the 112th day, complete healing of the ulcer was observed in 100 % of animals in the Am-Gl group, in 70 % in the Am and Gl groups. There was no complete healing of the ulcer in the SVF-CGl and Am-SVF groups. The greatest reduction in the area of the total altered skin from 21 to 112 days of the experiment was noted in the groups Gl-SVF (34.7 %), K (31.6 %), Am-SVF (30.7 %). In the Am-Gl and Am groups, a reduction in the area of the total altered skin was recorded by 24.6 % and 14.7 %, respectively. In the GL group, the reduction in the area of the total altered skin was the smallest, by only 13.5 %.
Conclusion: The use of a decellularized human amniotic membrane fixed with medical adhesive BF-6 can be considered as a promising method for the conservative treatment of LRI of the skin.
Keywords: local radiation injury, transplantation, decellularized human amniotic membrane, efficacy, rat
For citation: Kobzeva IV, Astrelina TA, Brunchukov VA, Brumberg VA, Rastorgueva AA, Suchkova YuB, Usupzhanova DYu, Malivanova TF, Nikitina VA, Lishchuk SV, Dubova EA, Pavlov KA, Tonkal YaV, Serova OF, Samoilov AS. Transplantation of Human Decellularized Amniotic Membrane in Local Radiation Injuries. Medical Radiology and Radiation Safety. 2022;67(6):5–11. (In Russian). DOI:10.33266/1024-6177-2022-67-6-5-11
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Поступила: 20.07.2022. Принята к публикации: 25.09.2022.