JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-11-15
N.Yu. Vorobyeva1,2, T.A. Astrelina1, E.I. Yashkina1,2, A.K. Chigasova3,
A.A. Osipov2, D.Yu. Usupzhanova1, I.V. Kobzeva1, Yu.B. Suchkova1,
V.A. Brunchukov1, A.A. Rastorgueva1, Yu.A. Fedotov1,2, A.S. Samoilov1,
A.N. Osipov1,2
Effect of a Humic-Fulvic Acid Preparation on the Quantitative Yield of Residual γH2AX Foci and Proliferative Activity in Irradiated Human Mesenchymal Stromal Cells
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
3 Institute of Biochemical Physics, Moscow, Russia
Contact person: N.Yu. Vorobyeva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To evaluate the influence of a humic-fulvic acid substance on the quantitative yield of residual foci of the DNA double-strand break (DSB) repair protein-marker - phosphorylated histone H2AX (γH2AX) and proliferation activity in a culture of human mesenchymal stromal cells (MSCs) 24, 48, and 72 h after exposure to X-ray radiation at doses of 2, 4 and 10 Gy.
Material and methods: Through 24 hours after incubation of MSCs with a substance of humic-fulvic acids (Humic Complex, OOO Sistema-BioTechnologies, Russia) at a dilution of 1/1000. Cells were irradiated on an X-ray biological device RUB RUST-M1 at a voltage of 200 kV, beam current 2×5 mA, aluminum filter 1.5 mm, absorbed dose rate 0.85 Gy/min. Immunocytochemical staining was used to quantify the residual γH2AX foci and the percentage of proliferating cells using antibodies to γH2AX and Ki-67 (a marker protein for cell proliferation), respectively. Statistical analysis of the obtained data was carried out using the statistical software package Statistica 8.0 (StatSoft). To assess the significance of differences between samples, Student’s t-test was used.
Results and conclusion: The conducted studies showed that on the cell model used and under the above experimental conditions, the humic-fulvic acid substance does not affect the efficiency of repair of radiation-induced DNA DSBs, however, it significantly reduces the proliferation activity of both irradiated and non-irradiated MSCs. It is advisable to conduct detailed studies of the molecular and cellular mechanisms of the antiproliferative effect of humic and fulvic acids.
Keywords: mesenchymal stromal cells, X-ray radiation, γH2AX, residual foci, DNA double-strand breaks, cell proliferation, humic acids, fulvic acids
For citation: Vorobyeva NYu, Astrelina TA, Yashkina EI, Chigasova AK, Osipov AA, Usupzhanova DYu, Kobzeva IV, Suchkova YuB, Brunchukov VA, Rastorgueva AA, Fedotov YuA, Samoilov AS, Osipov AN. Effect of a Humic-Fulvic Acid Preparation on the Quantitative Yield of Residual γH2AX Foci and Proliferative Activity in Irradiated Human Mesenchymal Stromal Cells. Medical Radiology and Radiation Safety. 2023;68(2):11–15. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-11-15
References
1. Nardi S., Schiavon M., Francioso O. Chemical Structure and Biological Activity of Humic Substances Define Their Role as Plant Growth Promoters. Molecules. 2021;26;8. doi: 10.3390/molecules26082256.
2. Klucakova M. Size and Charge Evaluation of Standard Humic and Fulvic Acids as Crucial Factors to Determine Their Environmental Behavior and Impact. Front Chem. 2018;6:235. doi: 10.3389/fchem.2018.00235.
3. Benderskii N.S., Kudelina O.M., Gantsgorn E.V., Safronenko A.V. Fulvic Acid: an Active Food Additive or Medication? Kuban Scientific Medical Bulletin. 2020;27;3:78-91. doi: 10.25207/1608-6228-2020-27-3-78-91.
4. Buzlama A.V., Chernov Iu N. [Humic Substances: Pharmacological Properties, Mechanisms of Action, and Prospects for Use in Medicine]. Eksp Klin Farmakol. 2010;73;9:43-48.
5. van Rensburg C.E. The Antiinflammatory Properties of Humic Substances: A Mini Review. Phytother Res. 2015;29;6:791-795. doi: 10.1002/ptr.5319.
6. Pustovalova M., Astrelina Т.A., Grekhova A., Vorobyeva N., Tsvetkova A., Blokhina T., et al. Residual γH2AX Foci Induced by Low Dose X-Ray Radiation in Bone Marrow Mesenchymal Stem Cells Do Not Cause Accelerated Senescence in the Progeny of Irradiated Cells. Aging. 2017;9;11:2397-2410. doi: 10.18632/aging.101327.
7. Tsvetkova A., Ozerov I.V., Pustovalova M., Grekhova A., Eremin P., Vorobyeva N., et al. γH2AX, 53BP1 and Rad51 Protein Foci Changes in Mesenchymal Stem Cells During Prolonged X-ray irradiation. Oncotarget. 2017;8;38:64317-64329. doi: 10.18632/oncotarget.19203.
8. Ulyanenko S., Pustovalova M., Koryakin S., Beketov E., Lychagin A., Ulyanenko L., et al. Formation of γH2AX and pATM Foci in Human Mesenchymal Stem Cells Exposed to Low Dose-Rate Gamma-Radiation. International Journal of Molecular Sciences. 2019;20;11:2645. doi: 10.3390/ijms20112645.
9. Krenning L., van den Berg J., Medema R.H. Life or Death after a Break: What Determines the Choice? Molecular cell. 2019;76;2:346-358. doi: 10.1016/j.molcel.2019.08.023.
10. Aliper A.M., Bozdaganyan M.E., Orekhov P.S., Zhavoronkov A., Osipov A.N. Replicative and Radiation-Induced Aging: a Comparison of Gene Expression Profiles. Aging. 2019;11;8:2378-2387. doi: 10.18632/aging.101921.
11. Ulyanenko S., Pustovalova M., Koryakin S., Beketov E., Lychagin A., Ulyanenko L., et al. Formation of GammaH2AX and pATM Foci in Human Mesenchymal Stem Cells Exposed to Low Dose-Rate Gamma-Radiation. International Journal of Molecular Sciences. 2019;20;11:2645. doi: 10.3390/ijms20112645.
12. Vorob’eva N.Y., Kochetkov O.A., Pustovalova M.V., Grekhova A.K., Blokhina T.M., Yashkina E.I., et al. Comparative Analysis of the Formation of gammaH2AX Foci in Human Mesenchymal Stem Cells Exposed to (3)H-Thymidine, Tritium Oxide, and X-Rays Irradiation. Bull. Exp. Biol. Med. 2018;166;1:178-181. doi: 10.1007/s10517-018-4309-1.
13. Grekhova A.K., Pustovalova M.V., Eremin P.S., Ozerov I.V., Maksimova O.A., Gordeev A.V., et al. Evaluation of the Contribution of Homologous Recombination in DNA Double-Strand Break Repair in Human Fibroblasts after Exposure to Low and Intermediate Doses of X-ray Radiation. Biology Bulletin. 2020;46;11:1496-1502. doi: 10.1134/s1062359019110037.
14. Bushmanov A., Vorobyeva N., Molodtsova D., Osipov A.N. Utilization of DNA Double-Strand Breaks for Biodosimetry of Ionizing Radiation Exposure. Environmental Advances. 2022;8. doi: 10.1016/j.envadv.2022.100207.
15. Banath J.P., Klokov D., MacPhail S.H., Banuelos C.A., Olive P.L. Residual GammaH2AX Foci as an Indication of Lethal DNA Lesions. BMC Cancer. 2010;10:4. doi: 10.1186/1471-2407-10-4.
16. Vorobyeva N.Y., Babayan N.S., Grigoryan B.A., Sargsyan A.A., Khondkaryan L.G., Apresyan L.S., et al. Increased Yield of Residual γH2AX Foci in p53-Deficient Human Lung Carcinoma Cells Exposed to Subpicosecond Beams of Accelerated Electrons. Bulletin of Experimental Biology and Medicine. 2022;172;6:756-759. doi: 10.1007/s10517-022-05472-9.
17. Babayan N.S., Guryev D.V., Vorobyeva N.Y., Grigoryan B.A., Tadevosyan G.L., Apresyan L.S., et al. Colony-Forming Ability and Residual Foci of DNA Repair Proteins in Human Lung Fibroblasts Irradiated with Subpicosecond Beams of Accelerated Electrons. Bulletin of Experimental Biology and Medicine. 2021;172;1:22-25. doi: 10.1007/s10517-021-05323-z.
18. Hseu Y.C., Lin E., Chen J.Y., Liua Y.R., Huang C.Y., Lu F.J., et al. Humic Acid Induces G1 Phase Arrest and Apoptosis in Cultured Vascular Smooth Muscle Cells. Environ Toxicol. 2009;24;3:243-258. doi: 10.1002/tox.20426.
19. Salehi M., Piri H., Farasat A., Pakbin B., Gheibi N. Activation of Apoptosis and G0/G1 Cell Cycle Arrest Along with Inhibition of Melanogenesis by Humic Acid and Fulvic Acid: BAX/BCL-2 and Tyr Genes Expression and Evaluation of Nanomechanical Properties in A375 Human Melanoma Cell Line. Iran J. Basic Med. Sci. 2022;25;4:489-496. doi: 10.22038/IJBMS.2022.60651.13444.
20. Yang H.L., Huang P.J., Chen S.C., Cho H.J., Kumar K.J., Lu F.J., et al. Induction of Macrophage Cell-Cycle Arrest and Apoptosis by Humic Acid. Environ Mol. Mutagen. 2014;55;9:741-750. doi: 10.1002/em.21897.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The analysis of residual foci was carried out with the support of the RNF (project No. 22-2400490).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.