JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 6
DOI:10.33266/1024-6177-2023-68-6-49-54
A.I. Kotikova, E.A. Blinova, A.V. Akleyev
Analysis of Vβ-Segment Diversity of T-Cell Receptor in Residents
of the Techa Riverside Villages Chronically Exposed to Radiation in the Long-Term Period
Urals Research Center for Radiation Medicine, Chelyabinsk, Russia
Chelyabinsk State University, Chelyabinsk, Russia
Contact person: Alisa Igorevna Kotikova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: Study the repertoire of the T-cell receptor in persons chronically exposed to radiation in the long-term period.
Material and methods: The study involved 48 people, who were divided into two groups: a group of exposed persons –
31 individuals with the average accumulated dose to red bone marrow (RBM) of 981±130 mGy, and a comparison group –
17 individuals, the average accumulated dose to RBM was 25.3±5.91 mGy. The study groups did not differ significantly in age, gender and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index.
Results: The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (p=0.03 and p=0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor is uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups by the Gini-TCR index (p=0.14).
Keywords: chronic radiation exposure, residents, the Techa river, peripheral blood, T-cell receptor, immunity
For citation: Kotikova AI, Blinova EA, Akleyev AV. Analysis of Vβ-Segment Diversity of T-Cell Receptor in Residents of the Techa Riverside Villages Chronically Exposed to Radiation in the Long-Term Period. Medical Radiology and Radiation Safety. 2023;68(6):49–54. (In Russian). DOI:10.33266/1024-6177-2023-68-6-49-54
References
1. Wang G.C., Dash P., McCullers J.A., Doherty P.C., Thomas P.G. T Cell Receptor αβ Diversity Inversely Correlates with Pathogen-Specific Antibody Levels in Human Cytomegalovirus Infection. Sci. Transl. Med. 2012;4;128:128ra42. doi:10.1126/scitranslmed.3003647.
2. Qi Q., Liu Y., Cheng Y., et al. Diversity and Clonal Selection in the Human T-Cell Repertoire. Proc. Natl. Acad. Sci. USA. 2014;111;36:13139-13144. doi:10.1073/pnas.1409155111.
3. Schrama D., Ritter C., Becker J.C. T Cell Receptor Repertoire Usage in Cancer as a Surrogate Marker for Immune Responses. Semin Immunopathol. 2017;39;3:255-268. doi:10.1007/s00281-016-0614-9.
4. McLean-Tooke A., Barge D., Spickett G.P., Gennery A.R. T Cell Receptor Vbeta Repertoire of T Lymphocytes and T Regulatory Cells by Flow Cytometric Analysis in Healthy Children. Clin. Exp. Immunol. 2008;151;1:190-198. doi:10.1111/j.1365-2249.2007.03536.x.
5. De Souza-Silva T.G., Gollob K.J., Dutra W.O. T-Cell Receptor Variable Region Usage in Chagas Disease: A Systematic Review of Experimental and Human Studies. PLoS Negl. Trop. Dis. 2022;16;9:e0010546. doi:10.1371/journal.pntd.0010546.
6. Fernández-Quintero M.L., Pomarici N.D., Loeffler J.R., Seidler C.A., Liedl K.R. T-Cell Receptor CDR3 Loop Conformations in Solution Shift the Relative Vα-Vβ Domain Distributions. Front Immunol. 2020;11:1440. doi:10.3389/fimmu.2020.01440.
7. Izrayelson M., Kasatskaya S., Pogorelyy M., Kirgizova V., Putintseva, E., Egorov.S., Britanova O.V., Chudakov D.M. Analysis of Individual Repertoires of T Cell Receptors. Immunologiya. 2016;37;6:347-352. doi: 10.18821/0206-4952-2016-37-6-347-352 (In Russ.).
8. Akleyev A.A., Dolgushin I.I. Immune Status of Persons with CRS at Later Time Points. Radiatsiya i Risk = Radiation and Risk. 2018;27;2:76-85. doi: 10.21870/0131-3878-2018-27-2-76-85 (In Russ.).
9. Rybkina V.L., Bannikova M.V., Adamova G.V., Dörr H., Scherthan H., Azizova T.V. Immunological Markers of Chronic Occupational Radiation Exposure. Health Phys. 2018;115;1:108-113. doi:10.1097/HP.0000000000000855.
10. Akiyama M. Late Effects of Radiation on the Human Immune System: an Overview of Immune Response among the Atomic-Bomb Survivors. Int. J. Radiat. Biol. 1995;68;5:497-508. doi:10.1080/09553009514551491.
11. Heylmann D., Rödel F., Kindler T., Kaina B. Radiation Sensitivity of Human and Murine Peripheral Blood Lymphocytes, Stem and Progenitor Cells. Biochimica et Biophysica Acta (BBA). Reviews on Cancer. 2014;1846;1:121–129. doi:10.1016/j.bbcan.2014.04.009.
12. Yoshida K., Misumi M., Kubo Y., Yamaoka M., Kyoizumi S., Ohishi W., Hayashi T., Kusunoki Y. Long-Term Effects of Radiation Exposure and Metabolic Status on Telomere Length of Peripheral Blood T Cells in Atomic Bomb Survivors. Radiat. Res. 2016;186:367–376. doi: 10.1667/RR14389.1.
13. Kyoizumi S., Yamaoka M., Kubo Y., et al. Memory CD4 T-Cell Subsets Discriminated by CD43 Expression Level in A-Bomb Survivors. Int. J. Radiat. Biol. 2010;86;1:56-62. doi:10.3109/09553000903272641.
14. Candéias S.M., Mika J., Finnon P., et al. Low-Dose Radiation Accelerates Aging of the T-Cell Receptor Repertoire in CBA/Ca Mice. Cell. Mol. Life Sci. 2017;74;23:4339-4351. doi:10.1007/s00018-017-2581-2.
15. Candéias S.M., Kabacik S., Olsen A.K., et al. Ionizing Radiation Does Not Impair the Mechanisms Controlling Genetic Stability During T Cell Receptor Gene Rearrangement in Mice. Int. J. Radiat. Biol. 2018;94;4:357-365. doi:10.1080/09553002.2018.1439195.
16. Seki H., Kanegane H., Iwai K., et al. Ionizing Radiation Induces Apoptotic Cell Death in Human TcR-Gamma/Delta+ T and Natural Killer Cells Without Detectable p53 Protein. Eur. J. Immunol. 1994;24;11:2914-2917. doi:10.1002/eji.1830241150.
17. Smirnova S.G., Orlova N.V., Zamulayeva I.A., Tkachenko N.P., Lozebnoy N.I., Kaplan M.A., Tumanov K.A., Kashcheyev V.V., Ivanov V.K., Sayenko A.S. Monitoring of the Frequency of Lymphocytes with Mutations at T-Cell Receptor Locus in Chernobyl Cleanup Workers. Radiatsiya i Risk = Radiation and Risk. 2012;21;1:20-29. (In Russ.).
18. Saenko A.S., Zamulaeva I.A., Smirnova S.G., et al. Determination of Somatic Mutant Frequencies at Glycophorin A and T-Cell Receptor Loci for Biodosimetry of Acute and Prolonged Irradiation. Appl. Radiat. Isot. 2000;52;5:1145-1148. doi:10.1016/s0969-8043(00)00061-0.
19. Blinova E.A., Veremeyeva G.A., Markina T.N., Akleyev A.V. Apoptosis of Blood Lymphocytes and Muta-Tions in the T-Cell Receptor of Late Time after Chronic Radiation Exposure in Humans. Voprosy Radiatsionnoy Bezopasnosti = Journal of Radiation Safety Issues. 2011;4:38-44 (In Russ.).
20. Akleyev A.A., Blinova E.A., Dolgushin I.I. TCR-Mutations in Peripheral Blood Lymphocytes and Immune Status in Individuals Exposed to Chronic Radiation Exposure. Rossiyskiy Immunologicheskiy Zhurnal = Russian Journal of Immunology. 2019;22;1:13-23. doi: 10.31857/S102872210005016-2 (In Russ.)
21. Smith L.E., Nagar S., Kim G.J., Morgan W.F. Radiation-Induced Genomic Instability: Radiation Quality and Dose Response. Health Phys. 2003;85;1:23-29. doi:10.1097/00004032-200307000-00006.
22. Van der Geest K.S., Abdulahad W.H., Horst G., Lorencetti P.G., Bijzet J., Arends S., van der Heiden M., Buisman A.M., Kroesen B.J., Brouwer E., Boots A.M. Quantifying Distribution of Flow Cytometric TCR-Vβ Usage with Economic Statistics. PLoS One. 2015;10;4:e0125373. doi: 10.1371/journal.pone.0125373.
23. Consequences of radioactive Contamination of the Techa River. Ed. Akleyev A.V. Chelyabinsk Publ., 2016. 390 p. (In Russ.).
24. Degteva M.O., Napye B.A., Tolstykh E.I., Shishkina E.A., Bugrov N.G., Krestinina L.Yu., Akleyev A.V. Individual Dose Distribution in Cohort of People Exposed as a Result of Radioactive Contamination of the Techa River. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2019;64;3:46-53 (In. Russ.). doi: 10.12737/article_5cf2364cb49523.98590475.
25. Kotikova A.I., Blinova E.A., Akleyev A.V. Subpopulation Composition of T-helpers in the Peripheral Blood of Persons Chronically Exposed to Radiation in the Long Term. Meditsina Ekstremalnykh Situatsiy = Extreme Medicine. 2022;24;2:65-73. doi: 10.47183/mes.2022.018.
26. Sanitary Rules and Regulations SanPin 2.6.1.2523-09. Standards of Radiation Safety (NRB - 99/2009). Moscow Publ., 2009. 225 p. (In Russ.).
27. Protocol of Analysis Beta Mark TCR Vβ Repertoire Kit, Beckman Coulter.
28. Huang L., Betjes M.G.H., Klepper M., Langerak A.W., Baan C.C., Litjens N.H.R. End-Stage Renal Disease Causes Skewing in the TCR Vβ-Repertoire Primarily within CD8+ T Cell Subsets. Front. Immunol. 2017;2017;8:1826. doi:10.3389/fimmu.2017.01826.
29. Kusunoki Y., Yamaoka M., Kasagi F., Hayashi T., MacPhee D.G., Kyoizumi S. Long-Lasting Changes in the T-Cell Receptor V Beta Repertoires of CD4 Memory T-Cell Populations in the Peripheral Blood of Radiation-Exposed People. Br. J. Haematol. 2003;122;6:975-984. doi:10.1046/j.1365-2141.2003.04520.x.
30. Goulielmaki M., Davanos N., Kogionou P., et al. The Impact of Radiation Therapy on the TCR Vβ Chain Repertoire in Patients with Prostate Cancer. Int. J. Oncol. 2022;60;6:71. doi:10.3892/ijo.2022.5361.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing.The work was carried out as part of the: “Study on the effect of chronic radiation exposure on the state of the human T-cell immune system”.
Contribution. Kotikova A.I. – methodology development, laboratory research, statistical processing, article authoring; Blinova E.A. – methodology development, article authoring; Akleyev A.V. – development of the research concept, scientific supervision, article authoring.
Article received: 20.07.2023. Accepted for publication: 27.08.2023.