JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2024. Vol. 69. № 4
DOI:10.33266/1024-6177-2024-69-4-13-19
D.T. Petrosova1, D.V. Uskalova1, O.V. Kuzmicheva1, V.O. Saburov3,
E.I. Sarapultseva1, 2
Enhancement of the Cytotoxic Effect of Proton Irradiation by Gold Nanoparticles
1 Obninsk Institute for Nuclear Power Engineering, Obninsk, Russia
2 National Research Nuclear University “MEPhI”, Moscow, Russia
3 A.F. Tsyb Medical Radiological Research Center, Obninsk, Russia
Contact person: D.T. Petrosova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To evaluate the bioefficiency and biosafety of proton irradiation in combination with gold nanoparticles (AuNPs) on a model of a higher invertebrate animal Daphnia magna from the suborder crustaceans in in vivo experiments.
Materials and methods: The synthesis of AuNPs was carried out by a single-stage method of femtosecond laser ablation. A laboratory culture of Daphnia magna (D. magna) was used. Animals were cultured in a climatostat (model P2). Biological parameters (viability, fertility and cytotoxicity) were evaluated in two consecutive generations (F0) and (F1). Only animals of the parental generation (F0) were exposed to acute irradiation with a scanning proton beam (energy 150 MeV) on the Prometheus proton complex. Viability and fertility D. magna were evaluated in a 21-day experiment on a daily basis. A total of 10 to 60 individuals were analyzed in control and experimental groups. Cytotoxicity was analyzed by an MTT test modified for studies of the effect on invertebrates in in vivo experiments. From 11 to 97 samples were analyzed for cytotoxicity. There were 20 animals in each sample. The results are analyzed by methods of mathematical statistics adjusted for multiple comparisons.
Results: Irradiation at doses of 10 and 30 Gy caused a decrease in animal viability, which was increased in 1.35 times. Reproductive dysfunction was found in both irradiated and first-generation animals. The use of AuNPs did not cause oxidative stress in D. magna, but increased the cytotoxic effect of proton irradiation. AuNPs contributed to the cytotoxic effect.
Conclusions: Since the results obtained are consistent with the data published in the cited articles on vertebrates, it is possible to assume a universal mechanism of cytotoxic effect of proton irradiation in combination with AuNPs on both invertebrates and vertebrates, including humans, and the possibility of using AuNPs as radiosensitizers to enhance the effect of irradiation in binary proton therapy technologies.
Keywords: Daphnia magna, protons, gold nanoparticles (AuNPs), viability, fertility, cytotoxicity, transgeneration effect
For citation: Petrosova DT, Uskalova DV, Kuzmicheva OV, Saburov VO., Sarapultseva E.I. Enhancement of the Cytotoxic Effect of Proton Irradiation by Gold Nanoparticles. Medical Radiology and Radiation Safety. 2024;69(4):13–19. (In Russian). DOI:10.33266/1024-6177-2024-69-4-13-19
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out with the financial support of the Russian Academy of Sciences within the framework of scientific project No. 23-24-10041. The irradiation was carried out on the equipment of the Central Research Center «Radiological and Cellular Technologies» of the Federal State Budgetary Institution «NMIC Radiology» of the Ministry of Health of the Russian Federation.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.03.2024. Accepted for publication: 25.04.2024.