JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
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The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
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The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 3
DOI:10.33266/1024-6177-2025-70-3-11-15
S.A. Korneva1, A.K. Chigasova1, 2, 3, A.A. Osipov2, M.A. Ignatov1, 2,
N.Yu. Vorobyova1, 2, V.O. Saburov4, E.I. Kazakov4, S.N. Koryakin4,
Yu.A. Fedotov1, 2, A.Yu. Bushmanov1, A.N. Osipov1, 2
Post-Irradiation Changes in the Number of γH2ax and patm Protein Foci in Human Mesenchymal Stem Cells Irradiated with 14.1 MeV Neutrons
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
3 Institute of Biochemical Physics, Moscow, Russia
4 A.F. Tsyb Medical Radiological Research Center, Obninsk, Russia
Contact person: A.N. Osipov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: Comparative analysis of dose-response dependences and kinetics of post-radiation changes in the number of γH2AX and pATM protein foci in human mesenchymal stem cells (MSCs) exposed to 14.1 MeV neutrons and cobalt-60 gamma-radiation.
Material and methods: The study used a primary culture of human MSCs obtained from the collection of BioloT LLC (Russia). The cells were irradiated using a neutron generator NG-14 (VNIIA, Russia), which provided neutron fluxes with an energy of 14.1 MeV, and a gamma-therapeutic device ROKUS-AM (JSC Ravenstvo, Russia; cobalt-60, dose rate 0.5 Gy/min) at doses of 0.1, 0.25 and 0.5 Gy. For quantitative assessment of γH2AX and pATM foci, immunocytochemical staining was done using antibodies to γH2AX and pATM, respectively. Statistical significance was assessed using analysis of variance (ANOVA).
Results: It was shown that the kinetics of post-radiation changes in the number of γH2AX foci in cells irradiated with neutrons is slower than after gamma irradiation. 24 h after irradiation with neutrons, ~ 62 % of γH2AX foci and ~ 52 % of pATM foci were recorded from their number 0.5 h after irradiation. These values were statistically significantly (p < 0.001) higher than the proportions of residual foci calculated after exposure to gamma-radiation: ~ 16 % and 6 %, respectively. The results obtained indicate that the proportion of complex, difficult-to-repair DNA damage in cells irradiated with neutrons is significantly higher than with gamma-radiation.
Keywords: mesenchymal stem cells, fast neutrons, gamma-radiation, γH2AX, рATM, DNA damage, DNA repair
For citation: Korneva SA, Chigasova AK, Osipov AA, Ignatov MA, Vorobyova NYu, Saburov VO, Kazakov EI, Koryakin SN, Fedotov YuA, Bushmanov AYu, Osipov AN. Post-Irradiation Changes in the Number of γH2AX and patm Protein Foci in Human Mesenchymal Stem Cells Irradiated with 14.1 MeV Neutrons. Medical Radiology and Radiation Safety. 2025;70(3):11–15. (In Russian). DOI:10.33266/1024-6177-2025-70-3-11-15
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The research was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (No. 1023112000035-8, code “Cosmos-DNA”).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.02.2025. Accepted for publication: 25.03.2025.