JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2025. Vol. 70. № 4
DOI:10.33266/1024-6177-2025-70-4-16-20
E.A. Mysina1, N.R. Popova1, A.E. Shemyakov1, 2, I.V. Savintseva1, N.N. Chukavin1, A.L. Popov1
Study of Proton Beam Influence on the Growth Dynamics and Viability of 3D Cell Spheroids Formed From 4T1 Carcinoma Cells
1 Institute of Theoretical and Experimental Biophysics, Pushchino, Russia
2 PTC LPI, Protvino, Russia
Contact person: A.L. Popov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Background: Proton therapy is considered one of the most promising methods in the treatment of complex localized tumors, but still has some shortcomings, which requires the development of new approaches to improve its effectiveness. One of the most promising approaches is the use of radiosensitizers that can enhance the radiation-induced effects of a proton beam. However, the use of 2D tumor cell models for screening potential radiosensitizers is insufficient for the effective translation of the experimental data to the in vivo level. 3D cellular spheroids are a convenient and relevant model for studying new approaches in the therapy of solid tumors, since they allow simulating the conditions of the microenvironment of tumor cells and simulating in vivo conditions, including the presence of an intercellular matrix and the formation of a certain zonality.
Purpose: To create an experimental model of a tumor spheroid based on 4T1 tumor cells irradiated with a proton beam for screening potential nanoradiosensitizers.
Material and methods: In vitro biological activity was assessed using a 4T1 cell line (mouse carcinoma) culture. The hanging drop method was used to form cell spheroids. The spheroids were irradiated with a proton beam at the Bragg peak on at a dose of 0–12 Gr using the “Prometheus” therapeutic proton complex . The clonogenic test was used to analyze the viability and mitotic activity of the cells after irradiation. The growth dynamics of irradiated 3D spheroids has been assessing by analyzing micromorphometry for 8 days after irradiation.
Keywords: cell spheroid, tumor model, hadron therapy, protons
For citation: Mysina EA, Popova NR, Shemyakov AE, Savintseva IV, Chukavin NN, Popov AL. Study of Proton Beam Influence on the Growth Dynamics and Viability of 3D Cell Spheroids Formed From 4T1 Carcinoma Cells. Medical Radiology and Radiation Safety. 2025;70(4):16–20. (In Russian). DOI:10.33266/1024-6177-2025-70-4-16-20
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The article was prepared within the framework of the Russian Science Foundation grant No. 22-73-10231, https://rscf.ru/project/22-73-10231 /.
Contribution. E.A. Mysina – work with spheroids (cultivation, irradiation, viability analysis), N.R. Popova – scientific text editing, A.E. Shemyakov – radiation and dosimetry at the proton therapeutic complex Prometheus, I.V. Savintseva – cell culture, N.N. Chukavin – scientific text editing, A.L. Popov – research design development, scientific guidance.
Article received: 20.03.2025. Accepted for publication: 25.04.2025.