JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
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The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2015. Vol. 60. No. 6. P. 15-19
RADIATION MEDICINE
T.A. Astrelina1, A.Yu. Bushmanov1, I.V. Kobzeva1, A.V. Akleyev2,3, G.P. Dimov2, P.S. Eremin1, A.S. Samoilov1
Evaluation of Skin Fibroblasts Proliferation and Secretion from Patients with Chronic Radiation Exposure
1. A.I. Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Urals Research Center for Radiation Medicine, Chelyabinsk, Russia; 3. Chelyabinsk State University, Russia
ABSTRACT
Purpose: To investigate skin fibroblasts proliferation and activity in patients with chronic radiation exposure.
Material and methods: The study of skin fibroblasts bioptates from patients with chronic radiation exposure (19 samples, the mean age was 66.1 ± 7.6 years) and healthy donors (15 samples). Culturing of skin fibroblasts were performed by standard methods. The average dose accumulated during chronic radiation exposure of the soft tissue was 0.03 ± 0.03 Gy in the study group, and 0.53 ± 0.75 Gy of the bone marrow. Study of proliferative activity of skin fibroblasts was carried out by Delta Cell Index for the normalized time point with the slope of the curve in the exponential growth phase using cell analyzer xCELLigence. Investigation of the secretory activity of skin fibroblasts (concentration vascular endothelial growth factor (VEGF), interleukin 6 (IL-6), brain-derived neurotrophic factor (BDNF), fibroblast growth factor (FGF) in the cultural medium were determined by ELISA kits (R & D Systems, USA).
Results: It is found that the normalized cell index delta of skin fibroblasts was higher in the skin fibroblasts of patients compared with the healthy donors (189.91 ± 21,35 EU and 108.67 ± 4.25 EU, respectively, p = 0.04), that is, the decrease of proliferative activity in the patients compared to the control group. The secretory activity of skin fibroblasts is different: the concentration level of VEGF and BDNF was higher in the healthy donors compared with patients -174.22 ± 12.21 and 86.37 ± 6.81 pg/ml, p = 0.04; 80.10 ± 7.22 and 12.66 ± 2.35 pg/ml, p = 0.03, respectively. The concentration of IL-6 in the culture medium of the healthy donors is lower than in the patients (26.45 ± 5.16 and 116.68 ± 10.01 pg/ml, p = 0.03). It was found that there is the correlation between the dose of chronic radiation exposure and the fibroblasts secretion activity and proliferative potential.
Conclusion: The dose accumulated during chronic radiation exposure affects the proliferation and activity of skin fibroblasts. The dose accumulated during chronic radiation exposure correlates with biochemical markers.
Key words: proliferation, secretion, skin fibroblasts, chronic radiation Exposure
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For citation: Astrelina TA, Bushmanov AYu, Kobzeva IV, Akleyev AV, Dimov GP, Eremin PS, Samoilov AS. Evaluation of Skin Fibroblasts Proliferation and Secretion from Patients with Chronic Radiation Exposure. Medical Radiology and Radiation Safety. 2015;60(6):15-9. Russian.
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