JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2019. Vol. 64. No. 5. P. 5–8
DOI: 10.12737/1024-6177-2019-64-5-5-8
G.M. Minkabirova, S.A. Abdullaev
Increase of Cell-Free Nuclear and Mitochondrial DNA Content in the Urine of Rats after X-ray Irradiation or Bleomycin Administration
Institute of Theoretical and Experimental Biophysics, Pushchino, Russia. Е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
G.M. Minkabirova – Junior Researcher;
S.A. Abdullaev – Research Fellow, PhD Biol.
Abstract
Purpose: To study the content of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear DNA (cf-nDNA) in urine of rats exposed to ionizing radiation, and after injection of a cytostatic drug bleomycin.
Material and methods: Wistar male rats aged 3 months were used in the experiments. Rats were irradiated at a doses of 3, 5, and 8 Gy. Bleomycin was administered intraperitoneally in concentrations of 3, 7, and 10 mg/kg. The DNA content was measured by real-time PCR.
Results: The results showed an increase in the level of the number of cf-nDNA and cf-mtDNA fragments in urine of irradiated rats. It was shown that the content of cf-nDNA and cf-mtDNA has a linear dependence on the X-ray dose. Thus, the maximum number of mtDNA and nDNA copies was recorded for 12–24th hours after irradiation. The number of PCR amplification products of cf-mtDNA is 2–3 times higher than those of cf-nDNA. Data analysis of the content of cf-nDNA and cf-mtDNA in rat urine after introduction of bleomycin also showed elevated levels compared with control animals. It was shown that the content of cf-nDNA and cf-mtDNA has a linear dependence on the dose of the chemotherapeutic drug.
Conclusion: Thus, it has been shown that it is possible to overcome the transrenal (renal) barrier in animals with cf-mtDNA and cf-nDNA and pass them into the urine after X-ray irradiation, as well as after the administration of bleomycin. The dose dependence of the identified effects was found. The increased content of cell-free DNA in the urine can be considered as a potential biomarker for assessing the level of genotoxic load during radiation damage to the body, as well as when exposed to other genotoxic agents.
Key words: cell-free DNA in urine, X-ray irradiation, bleomycin, rats
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For citation: Minkabirova GM, Abdullaev SA. Increase of Cell-Free Nuclear and Mitochondrial DNA Content in the Urine of Rats after X-ray Irradiation or Bleomycin Administration. Medical Radiology and Radiation Safety. 2019;64(5):5-8. (in Russian).
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