Medical Radiology and Radiation Safety. 2017. Vol. 62. No. 3. P.13-16

DOI: 10.12737/article_5926b8549d17e4.90563872

Extract of Lecanicillium Lecanii Fungus Suppress Thymocyte Apoptosis after Irradiation

M.V. Bibikova¹, I.A. Spiridonova¹, A.F. Korystova², L.N. Kublik², M.Kh. Levitman², V.V. Shaposhnikova², Yu.N. Korystov²

1. LLC «VIORIN», Moscow, Russia; 2. Institute of Theoretical and Experimental Biophysics of RAS, Moscow region, Pushchino. Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

M.V. Bibikova - General Director LLC «VIORIN», Dr. Sc. Biol.; I.A. Spiridonova - Senior Researcher, PhD Biol.; A.F. Korystova - Senior Researcher; L.N. Kublik - Senior Researcher, PhD Biol.; M.Kh. Levitman - Senior Researcher, PhD Biol.; V.V. Shaposhnikova - Senior Researcher, PhD Biol.; Yu.N. Korystov - Head of Lab., Dr. Sc. Biol.

Abstract

Purpose: The effects of Lecanicilium lecanii fungus extract on 15-lipoxygenase activity and rat thymocyte apoptosis after irradiation were studied.

Material and methods: Lecanicilium lecanii fungus was extracted with acetone then acetone was evaporated and sediment was dissolved in ethanol. Wistar rat thymocytes were irradiated with X-rays at a dose of 6 Gy and incubated 6 h in nutrition medium. Apoptosis of thymocytes was recorded by nucleus damage and DNA fragmentation. 15-lipoxygenase activity was determined with linoleic acid oxidation.

Results: Fungus extract suppressed 15-lipoxygenase activity. Extract concentration that inhibited 15-lipoxygenase activity by 50 % (IC₅₀) = 15 µg/ml. It was shown that the share of damaged nuclei increased to 67 % after irradiation of thymocytes. The addition of the extract immediately after irradiation decreased the percent of damaged nuclei and DNA fragmentation in irradiated thymocytes. The effect increased with growth of extract concentration and in a dose of the extract 50 µg/ml percent of damaged nuclei in irradiated thymocytes decreased to control (16 %) and DNA fragmentation up to level below controlling one. IC₅₀ of extract on nucleus damage was 6 µg/ml.

Conclusion: These data show that Lecanicilium lecanii fungus extract contains effective 15-lipoxygenase inhibitor.

Key words: apoptosis, thymocytes, radiation, inhibitors, 15 lipoxygenase

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For citation: Bibikova MV, Spiridonova IA, Korystova AF, Kublik LN, Levitman MKh, Shaposhnikova VV, Korystov YuN. Therapy of Experimental Neurosis in Rabbits by Using the Electromagnetic Field in Comparison with Electrical and Chemical Nature. Medical Radiology and Radiation Safety. 2017;62(3):13-6. Russian. DOI: 10.12737/article_5926b8549d17e4.90563872

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