Medical Radiology and Radiation Safety. 2015. Vol. 60. No. 2. P. 37-46

RADIATION MEDICINE

V.Yu. Nugis, M.G. Kozlova

Cytogenetic Researches in Two Situations of Unregulated Ionizing Radiation Source Finding

Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

ABSTRACT

Purpose: Evaluation of the results of cytogenetic studies aimed for biological dose indication for two groups of people probably irradiated with unregulated sources of ionizing radiation.

Material and methods: The cytogenetic studies were carried out with use of peripheral blood lymphocyte cultures, FISH-method was applied in one case and classic method of chromosome staining was applied in another case.

Results: It is shown that chromosome aberration frequencies in almost all examined people did not exceed limits of background values despite real existence of ionizing radiation sources. The frequency of FISH-registered translocations exceeded a reference level and corresponded to a dose of 0.34 Sv only in one individual. However his involvement into the radiation situation is doubtful.

Conclusion: Cytogenetic data received with use of classical or FISH-methods of chromosome painting corresponded to low doses estimated with physical methods for persons involved in two considered radiation incidents.

Key words: chromosome aberrations, peripheral blood lymphocytes culture, occasional irradiation

REFERENCES

1. Cytogenetic Analysis for Radiation Dose Assessment: A Manual. Vienna, International Atomic Energy Agency, 2001. Technical Reports series No. 405. 126 p.
2. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies. Vienna: IAEA. 2011. 245 p.
3. Koterov A.N. Ot ochen' malykh do ochen' bol'shikh doz radiatsii: novye dannye po ustanovleniyu diapazonov i ikh eksperimental'no-epidemiologicheskie obosnovaniya. Medical Radiology and Radiation Safety. 2013. Vol. 58. No. 2. P. 5-21.
4. Lucas J.N., Awa A., Straume T. et al. Rapid translocation frequency analysis in human decades after exposure to ionizing radiation. Int. J. Radiat. Biol. 1992. Vol. 62. No. 1. P. 53-63.
5. Mendelsohn M.L., Mayall B.H., Bogart E. et al. DNA content and DNA-based centromeric index of 24 human chromosomes. Science. 1973. Vol. 179. No. 78. P. 1126-1129.
6. Normy radiatsionnoi bezopasnosti (NRB-99/2009). Sanitarnye pravila i normativy SanPinN 2.6.1.2523-09. Moscow. 2009. 70 p.
7. Lloyd D.C., Edwards A.A., Moquet J.E. et al. Doses in radiation accidents investigated by chromosome aberration analysis. XXI: Review of cases investigated, 1994-1996. National Radiological Protection Board. 1996. NRPB-R291. 24 p.
8. Voisin P., Roy L., Banderitter M. Why can’t we find a better biological indicator of dose? Radiation Protection Dosimetry. 2004. Vol. 112. No. 4. P. 465-469.
9. Edwards A.A., Lindholm C., Darroudi F. et al. Review of translocations detected by FISH for retrospective biological dosimetry application. Radiat. Protect. Dosimetry. 2005. Vol. 113. No. 4. P. 396-402.
10. Ainsbury E.A., Bakhanova E., Barquinero J.F. Review of retrospective dosimetry techniques for external ionising radiation exposures. Radiat. Protect. Dosimetry. 2011. Vol. 147. No. 4. P. 573-592.
11. Snigireva G.P., Bogomazova A.N., Novitskaya N.N. et al. Biologicheskaya indikatsiya radiatsionnogo vozdeistviya na organizm cheloveka s ispol'zovaniem tsitogeneticheskikh metodov. Meditsinskaya tekhnologiya FS-2007/015-U. Moscow. 2007. 29 p.
12. Vorobtsova I.E., Taker Dzh.D., Timofeeva I.M. et al. Vliyanie vozrasta i oblucheniya na chastotu translokatsii i ditsentrikov, opredelyaemykh metodom FISH, v limfotsitakh cheloveka. Radiats. biologiya. Radioekologiya. 2000. Vol. 40. No. 2. P. 142-148.
13. Whitehouse C.A., Edwards A.A., Tawn E.J. et al. Translocation yields in peripheral blood lymphocytes from control populations. Int. J. Radiat. Biol. 2005. Vol. 81. No. 2. P. 139-145.
14. Sevan'kaev A.V., Khvostunov I.K., Snigireva G.P. et al. Sravnitel'nyi analiz rezul'tatov tsitogeneticheskikh obsledovanii kontrol'nykh grupp lits v razlichnykh otechestvennykh laboratoriyakh. Radiats. biologiya. Radioekologiya. 2013. Vol. 53. No. 1. P. 5-24.

For citation: Nugis VYu, Kozlova MG. Cytogenetic Researches in Two Situations of Unregulated Ionizing Radiation Source Finding. Medical Radiology and Radiation Safety. 2015;60(2):37-46. Russian.

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