Medical Radiology and Radiation Safety. 2018. Vol. 63. No. 1. P. 35-39

RADIATION BIOLOGY

DOI: 10.12737/article_5a82eb9a9bbac1.67972336

Investigation of the Influence of the Conditioning Medium Factors Obtained During the Cultivation of Bone Marrow Mesenchymal Stem Cells on the Course of Severe Local Radiation Injuries of Skin in Rats

A.A. Temnov¹, T.A. Astrelina¹, K.A. Rogov², V.G. Lebedev¹, T.A. Nasonova¹, A.V. Lyrschikova¹, Y.B. Deshevoy¹, O.A. Dobrynina¹, A.V. Melerzanov³, A.S. Samoylov¹, A.Yu. Bushmanov¹, B.B. Moroz¹

1. A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Research Institute of Human Morphology; Moscow, Russia; 3. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow region, Russia

A.A. Temnov - Leading Researcher, Dr. Sc. Med.; T.A. Astrelina - Head of Center for Biomedical Technologies, Dr. Sc. Med.; K.A. Rogov - Leading Researcher, Dr. Sc. Med., Prof.; V.G. Lebedev - Leading Researcher, PhD Biol.; T.A. Nasonova - Leading Researcher, PhD Med.; Yu.B. Deshevoy - Leading Researcher, PhD Med.; A.V. Lyrschikova - Senior Researcher, PhD Biol.; O.A. Dobrynina - Research Worker; A.V. Melerzanov - Head of Cells Center of MPhTI, PhD Med.; A.S. Samoylov - Director General, Dr. Sc. Med.; A.Yu. Bushmanov - First Deputy Director General, Dr. Sc. Med., Prof.; B.B. Moroz - Head of Lab., Academician of RAS

Abstract

Purpose: Study of the effect of paracrine factors, produced by MMSC of bone marrow during the cultivation, on the severity of local radiation injuries in the conditions of application in the early periods after irradiation.

Material and methods: Experiments were performed on rats of the breed Wistar weighing 280 g. Rats were exposed locally in iliolumbar region of the back using X-ray machine LNC-268 (RAP 100-10) at a dose of 110 Gy (30 kV tube voltage, current 6.1 mA, filter Al 0.1 mm thick), dose rate is 21.4 Gy/min. Area of the irradiation field was 8.2-8.5 cm². The conditioned medium obtained by culturing MMSC of rats’ bone marrow was administered in dose 1.0 ml (total protein 8 mg/ml) at 1, 3, 6, 8 and 10 days after irradiation. The severity of radiation damage to the skin and the effects of therapy were evaluated in dynamics by clinical manifestations, using planimetry and histological methods.

Results: It was shown that in control animals and in rats, with the introduction of the conditioned medium, the values of the skin lesion area in the period up to the 29th day after irradiation practically did not differ, gradually decreasing in control animals from 5.9 ± 0.6 cm² to 2.2 ± 0.3 cm² at the 15th and 29th days after irradiation, respectively. Then, in the control group, the lesion area ranged from 1.4 ± 0.6 cm² on the 50th day to 1.9 ± 0.8 cm² on the 71st day. In the experimental group of animals, with the introduction of factors of the conditioning medium, a decrease in the area of the lesion and a stable dynamics of healing of radiation ulcers, beginning from the 36th day, there was a gradual decrease in the area of the lesion, which reached 0.2 ± 0.1 cm² by the 71st day after irradiation. On the 64-71th day after irradiation, the difference between the areas of skin lesion in the experimental and control groups was statistically significant, p <0.05. The histological analysis showed that the use of paracrine factors obtained from MMSC in the process of cultivation significantly reduces the severity of the inflammatory reaction and accelerates the regeneration processes.

Conclusion: Thus, the introduction of conditioned medium factors obtained during the cultivation of mesenchymal stem cells of the bone marrow facilitates a more easy flow of the pathological process and the healing of radiation ulcers after local radiation damage to the skin of rats. Apparently, the favorable effect of paracrine factors introduced in the early periods after irradiation, with severe local radiation injuries, is associated with their effect on pathological processes in the inflammatory-destructive stage.

Key words: paracrine factors, conditioned medium, cell technology, local radiation injuries, mesenchymal stem cells, bone marrow, radiation skin ulcers, rats

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For citation: Temnov AA, Astrelina TA, Rogov ˀ, Lebedev VG, Nasonova TA, Lyrschikova AV, Deshevoy YB, OA Dobrynina, Melerzanov AV, Samoylov AS, Bushmanov AYu, Moroz BB. Investigation of the Influence of the Conditioning Medium Factors Obtained During the Cultivation of Bone Marrow Mesenchymal Stem Cells on the Course of Severe Local Radiation Injuries of Skin in Rats. Medical Radiology and Radiation Safety. 2018;63(1):35-9. DOI: 10.12737/article_5a82eb9a9bbac1.67972336. Russian.

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