Medical Radiology and Radiation Safety. 2014. Vol. 59. No. 6. P. 5-17


F.S. Ambesi-Impiombato1, A.A. Ivanov2,3,4, A. Mancini5, O.V. Belov4, A. Borrelli5, A.G. Molokanov6, T.M. Bulynina2,3, S.V. Vorozhtsova2,3, A.N. Abrosimova2,3, E.A. Krasavin4

Effect of Recombinant Manganese Superoxide Dismutase (rMnSOD) on the Hematologic Status in Mice Irradiated by Protons

1. Department of Medical and Biological Sciences, University of Udine, Udine, Italy; 2. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 3. A.I. Burnazyan Federal Medical and Biophysical Center of FMBA, Moscow, Russia; 4. Laboratory of Radiation Biology, Joint Institute for Nuclear Research, Dubna, Russia; 5. Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, “Istituto Nazionale Tumori “ Fondazione G. Pascale” — IRCCS, Naples, Italy; 6. Laboratory of Nuclear Problems, Joint Institute for Nuclear Research, Dubna, Russia


Purpose: To study therapeutic properties of the human recombinant Manganese Superoxide Dismutase (rMnSOD) in mice exposed to the proton radiation.

Material and methods: Using the model of sublethal whole-body irradiation with protons available at Phasotron of the Joint Institute for Nuclear Research (Dubna, Russia), we reconstructed the bone-marrow form of the acute radiation syndrome in mice to test the therapeutic effect of rMnSOD. Male C57Bl/6 SPF mice with the average weight of 24 g were exposed to 171 MeV proton beam at the dose of 4 Gy and then subcutaneously treated with either rMnSOD or physiological solution.

Results: After irradiation, the daily subcutaneous treatment with rMnSOD for 6 days, has provided a statistically significant acceleration of the recovery of thymus and spleen mass and of the number of leukocytes in mice peripheral blood. In contrast mock-treated, irradiated control mice did not demonstrate these positive effects at day seven after exposure. The number of nucleated cells in the bone marrow of irradiated mice has even exceeded its basal level in the control group seven days after irradiation. The rMnSOD-treated group has thus demonstrated a significant hyper restoration of their normal characteristic.

Conclusion: The results on the state of organs of the immune system and hemopoiesis indicate a significant therapeutic effect of rMnSOD in treating the acute radiation disease induced by a sublethal dose of proton irradiation.

Key words: superoxidedismutase, rMnSOD, proton radiation, acute radiation syndrome, hematopoiesis, experimental therapy