Medical Radiology and Radiation Safety. 2018. Vol. 63. No. 2. P. 15-17
RADIATION BIOLOGY
DOI: 10.12737/article_5ac6190e95da25.42157674
Dependence of Body Weight on Age for Random-Bred Albino Rat and for Eight Lines of Laboratory Rat: Synthetic Studies of Data from Experimental Works and Nurseries in Aspect of the Relationship with Radiosensitivity. Some Characteristics of Rat Species
A.N. Koterov1, L.N. Ushenkova1, E.S. Zubenkova1, A.A. Wainson2, A.P. Biryukov1, A.S. Samoylov1
1. A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. N.N. Blokhin National Medical Research Center, Moscow
Abstract
For random-bred albino rat and for eight most known rat lines (Wistar, Wistar Hannover, Wistar Kyoto, Sprague Dawley, Lewis, Fisher 344, Lister and Long-Evans) a brief review of the origins and features was made, and data on the age-weight dependences in norm obtained from experimental works and presented in the materials of firms and nurseries were analysed. The data extracted from the sources by digitizing the original curves or taken from there from the tables were combined (Mean ± 95% Confidence Intervals), and the values were compared in parallel along the Student’s t-test and the Mann-Whitney U-test.
For half the rat lines (males and females) it was found that the body weight growth in works and nurseries does not coincide (statistically significant or in the form of distinct trends), and the discrepancy can began either from a certain time moment (Wistar Hannover, Sprague Dawley), or almost immediately after birth (Lewis, Long-Evans).
The detected phenomenon has practical significance for the object selection for radiosensitivity investigation. Differences in age at the same weight of animals in the experiment and in nurseries can cause errors in background radioresistance. A review of the studies on dependence of the radiosensitivity on the age of irradiated rats was performed with the reproduction of a number of published data in a graphic form and it was concluded that a mistake in the age of rats even for a few weeks can strongly affect the radiosensitivity. It is noted that the importance of taking into account the body mass index is due to the dependence on it of the mass of internal organs, the magnitude of which is affected, among other things, on the results of internal dosimetry.
Distribution by growth intensity (an age of achievement of weight 200 g) for males is follows: Wistar > Sprague-Dawley = Lister > Long-Evans (from nurseries) > Wistar Hannover > Lewis > Wistar Kyoto > Fisher 344 > Long-Evans (from works) > Wistar from 1906-1932 > random-bred albino.
As a result of the study, standard, tabular growth curves for random-bred rat and eight mentioned rat strains obtained by combining and statistical processing of data from all available sources were also presented. This material continues the traditions of Donaldson’s Tables (H.H. Donaldson, 1915) and the growth standards for laboratory animal lines in work of S.M. Poiley (1972).
The report of the individual data by some characteristics of a rat species is presented: average life expectancy, age and weight for various physiological periods of the development, and also a certain ‘standard’ weight for a rat as a species.
Key words: random-bred and pure-bred rat strains, Wistar, Wistar Hannover, Wistar Kyoto, Sprague Dawley, Lewis, Fisher 344, Lister, Long-Evans, standard weight growth curve, age-dependent radiosensitivity
CONTENT
1. Introduction: a brief historical review, the actualityof the problem, the formation of the aim and tasks of thestudy
1.1. The origins of the use of rats in biology and medicine, in particular, in radiobiology
1.2. The history of breading of the first laboratory lines of rats. Wistar and others
1.3. Ancestral source of linear rats and their genetics
1.4. Attempts to standardize the lines of rats by body weight and internal organs. Donaldson’s Tables of 1915 and later reference materials
1.5. The need to unify rodents by age and body weight for radiobiological experiments and for synthetic studies
1.6. Purpose and objectives of the study
2. Materials and methods
2.1. The investigated lines of rats
2.2. Used literary and commercial sources
- Experimental studies
- Prospectuses, catalogs and websites of firms and nurseries
2.3. The method of extraction and processing of primary data
2.4. Statistical processing and presentation of final data
2.5. Conflict of interest and the possibility of subjective biases
3. The results of the combined analysis of the data for age and body weight relationship for random-bred and linear rats
3.1. Random-bred white rats
3.2. Wistar. The ‘traditional’ line and Wistar Hannover and Wistar Kyoto sub-lines
- The origins of lines and their features
- Parameters of the Wistar line in experimental works of different periods and in nurseries
- Comparison of the dynamics of body weight growth for the Wistar Hannover and Wistar Kyoto lines in experimental works and in nurseries
- Comparison of the relationship between age and body weight for the Wistar, Wistar Hannover and Wistar Kyoto lines
3.3. Sprague-Dawley
- The origins of the line and its features
- Comparison of growth curves for experimental works and nurseries
3.4. Lewis
3.5. Fisher (Fisher 344; F344)
3.6. Lister
3.7. Long-Evans
- The origins of the line and the features of its use
- Comparison of growth curves for experimental works and nurseries
4. What rats are growing faster: comparison of age for reaching the reference value of body weight by random-bred and linear rats
5. Some characteristics of the rat species
5.1. Age periods of life Average life time Age periods
5.2. What is the value for the average body weight of a rat?
Conclusion
List of literature (198 sources, for Russian sources there is a translation into English)
Tables (16)
Figures (15)
For citation: Koterov AN, Ushenkova LN, Zubenkova ES, Wainson AA, Biryukov AP, Samoylov AS. Dependence of Body Weight on Age for Random-Bred Albino Rat and for Eight Lines of Laboratory Rat: Synthetic Studies of Data from Experimental Works and Nurseries in Aspect of the Relationship with Radiosensitivity. Some Characteristics of Rat Species. Medical Radiology and Radiation Safety, official website [Internet]. 2018 Apr. [cited 2018 Apr. 24];63(2):[about 41 p.]. Available from: http://medradiol.ru/journal_medradiol/abstracts/2018/2/15_Koterov_full.pdf. Russian. DOI: 10.12737/article_5ac6190e95da25.42157674
PDF (RUS) Full-text article (in Russian)