Medical Radiology and Radiation Safety. 2012. Vol. 57. No. 6. P. 51-57


E.V. Klisho, I.V. Kondakova, Е.L. Choinzonov, D.А. Shishkin, S.Yu. Chizhevskaya

Relation of the Level of Matrix Metalloproteinases and Their Tissue Inhibitors in Blood Serum to the Radiation Therapy Efficiency of Patients with Squamous Cell Head and Neck Cancer

Cancer Research Institute, SB of RAMS, Tomsk, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.


Purpose: To study of the levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in blood serum of patients with squamous cell head and neck carcinoma for identification of molecular markers and to create the mathematical model for predicting tumor response to radiotherapy.

Material and methods: Serum MMP and TIMP levels were determined by ELISA (enzyme-linked immunosorbent assay).

Results and conclusions: In patients with squamous cell head and neck carcinoma, serum TIMP-1 (133.54 ± 9.50 ng/mL) and TIMP-2 (88.39 ± 15.25 ng/mL) levels were significantly lower in the group of patients with complete tumor response to radiotherapy than those in patients without any response (193.24 ± 22.68 and 125.94 ± 4.80 ng/mL, respectively, р <0.01). Mathematical models for predicting response to radiotherapy were devised. Serum levels of TIMP-1, TIMP-2 and MMP-2 before the treatment and the primary tumor size were the most important predictors of radiotherapy response. The sensitivity, specificity and diagnostic accuracy of the discriminant model were 96 %, 75 % and 89 %, respectively.

Key words: squamous cell head and neck carcinomas, molecular markers, efficacy of radiotherapy, matrix metalloproteinases, tissue inhibitors