Medical Radiology and Radiation Safety. 2021. Vol. 66. № 4. P. 13–17

Comparative Evaluation оf the Anti-Radiation Efficacy of Flagellin
by Survival and Micronucleus Test

N.I. Lisina, K.Yu. Romanova, L.P. Sycheva, L.M. Rozhdestvensky

A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia

Contact person: Nina Ivanovna Lisina: This email address is being protected from spambots. You need JavaScript enabled to view it.  

ABSTRACT

Purpose: Comparative evaluation of the effectiveness of the domestic drug flagellin (development of the State Research Institute of High-Purity Biological Products, St. Petersburg) in an extended range of drug administration periods before and after irradiation, as well as evaluation of the possibility of using the micronucleus test as a biomarker of its effectiveness.

Material and methods: The work was performed on male ICR CD1 mice weighing 20–22g. The radioprotective effectiveness of flagellin was evaluated by the 30-day survival rate of experimental animals in comparison with control groups. The cytogenetic effect was evaluated by a micronucleus test in polychromatophilic erythrocytes (MJ-PCE) of mouse bone marrow. Irradiation was performed on the RUST M1 X-unit at a dose rate of 1.1 Gy/min in the dose range from 7 to 10 Gy for survival assessment and at a dose of 1 Gy for the micronucleus test. Flagellin was administered intraperitoneally at 0.2 mg/kg for 18 h and 30 min before irradiation, 10 and 30 min after irradiation. The animals of the control groups were injected with a solvent – phosphate-albumin buffer at the same time and in the same volume.

Results: The most effective was the use of flagellin for 30 min and 10 min in relation to radiation (survival at 8.5 Gy 92 % and 78 %, at 9 Gy 81 % and 55 %, respectively) with survival in the control 29 % and 0. In the most studied version of the introduction of flagellin 30 minutes before exposure, the FID values at the level of generally accepted estimates LD16, LD50, LD84, were 1.3; 1.2 and 1.2, respectively.

Conclusions: It is of interest to further expand the time range of the use of flagellin, especially after irradiation. The use of the method of estimating the proportion of polychromatophilic erythrocytes with micronuclei in the bone marrow allowed us to identify the optimal timing of drug administration much faster than in terms of survival (MJ-PCR test was performed 24 hours after irradiation). The MJ-PHE indicator can be considered as a potential biomarker of drug-induced increased radioresistance (this was previously shown in our studies for betaleukin, indralin, and riboxin).

Key words: anti-radiation drugs, flagellin, X-radiation, mice, radioprotective efficacy, micronucleus test, polychromatophilic erythrocytes

For citation: Lisina NI, Romanova KYu, Sycheva LP, Rozhdestvensky LM. Comparative Evaluation of the Anti-Radiation Efficacy 

of Flagellin by Survival and Micronucleus Test. Medical Radiology and Radiation Safety. 2021;66(4):13-17.

DOI: 10.12737/1024-6177-2021-66-4-13-17

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Conflict of interest. The authors declare no conflict of interest.

Financing. The study had no sponsorship.

Contribution. Article was prepared with equal participation of the authors.

Article received: 16.03.2021. 

Accepted for publication: 21.04.2021.