Medical Radiology and Radiation Safety. 2022. Vol. 67. № 5

DOI: 10.33266/1024-6177-2022-67-5-5-9

F.E. Ismailova, S.E. Nagieva, E.R. Nagiev

Research of the Activity of Glutathionperoxidase and the Content of Glutathion in Various Structural and Functional Divisions of the Radrain Brain when Exposed to Radiation

Dagestan State Medical University, Makhachkala, Russia

Contact person: E.R. Nagiev, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

ABSTRACT

Purpose: To study the specific content (per 1 g of tissue) of reduced glutathione and the activity of the enzyme of antioxidant protection glutathione peroxidase conjugated with it in various structural and functional parts of the rat brain when exposed to ionizing radiation at a dose of 6 Gy.

Material and methods: The studies were carried out on white outbred rats subjected to a single total γ-radiation at an average lethal dose of 6 Gy. The content of reduced glutathione and the activity of glutathione peroxidase were determined in various structural and functional parts of the brain of irradiated animals: cortex, brain stem, and cerebellum. A group of non-irradiated intact rats served as a control.

Results: In control animals, the activity of glutathione peroxidase in the cytoplasm is significantly higher than in mitochondria obtained from cells of the cortex, cerebellum, and brain stem. The content of reduced glutathione in control rats is highest in the cerebral cortex, followed by the cerebellum and brainstem. Significant violations of the studied biochemical parameters in the cerebral cortex, brainstem and cerebellum were found in the dynamics of radiation damage. Thus, in particular, the content of reduced glutathione in the cerebral cortex of rats on the 7th day after radiation exposure decreases to approximately 66 % compared with the indicators of the control group of animals.

Conclusions: Exposure to radiation at a dose of 6 Gy leads to a decrease in the content of the antioxidant glutathione in various structural and functional parts of the rat brain, especially on the 7th day after radiation injury. The detected changes in the glutathione content in the dynamics of acute radiation sickness correlate with changes in the activity of glutathione peroxidase, an enzyme directly coupled to this antioxidant.

Key words: glutathione, glutathione peroxidase, ionizing radiation, brain, cortex, brain stem, cerebellum, rats

For citation: Ismailova FE, Nagieva SE, Nagiev ER. Research of the Activity of Glutathionperoxidase and the Content of Glutathion in Various Structural and Functional Divisions of the Radrain Brain when Exposed to Radiation. 2022;67(5):5–9. (In Russian). DOI: 10.33266/1024-6177-2022-67-5-5-9

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Conflict of interest. The authors declare no conflict of interest.

Financing. The study had no sponsorship.

Contribution. Article was prepared with equal participation of the authors.

Article received: 20.06.2022. Accepted for publication: 25.08.2022.