Medical Radiology and Radiation Safety. 2022. Vol. 67. № 5
DOI: 10.33266/1024-6177-2022-67-5-18-23
V.P. Mamina
RADIOPROTECTIVE EFFECT OF THE ERACOND
ON SPERMATOGENESIS UNDER THE INFLUENCE OF A SINGLE EXTERNAL ACUTE γ-IRRADIATION
Institute of Plant and Animal Ecology, Ural Division of Russian Academy of Sciences, Ekaterinburg, Russia
Contact person: Vera Pavlovna Mamina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: Experimental evaluation of the radioprotective effect of Erakond on spermatogenesis in BALB/c mice under external acute γ-irradiation.
Material and methods: Single external γ-irradiation of males at a dose of 1 Gy was carried out at the IGUR facility (137Cs, dose rate 0.85 Gy/min). Erakond was administered orally for 6 days followed by irradiation 2 weeks later. Violation of spermatogenesis and its correction by eracondas in irradiated animals was assessed by the morphofunctional state of the testes, spermatozoa and the reproductive function of males.
Results: In mice on days 16, 24, 48 after irradiation, the number of seminiferous tubules with destructive changes in the spermatogenic epithelium increases from 2.4 to 5.4; 10.2 8.3 % respectively. Erakond contributed to the reduction in the number of seminiferous tubules with destruction to 3.2; 5.1 and 3.5 % respectively.
On the 24th, 48th day after irradiation, the number of spermatozoa with an abnormal head increases from 2.8 to 4.8 and 4.5 %; and tail pathology from 4.2 to 8.2 and 7.5 %, respectively, the number of live spermatozoa decreases from 58.1 to 40.5 and 20.0 %, respectively. Erakond contributed to a decrease in the number of spermatozoa with an abnormal head to 3.0; 3.5 %, with tail pathology up to 6.1; 5.3 % and an increase in the number of live sperm up to 50.8; 35.5 % respectively.
In mice, on days 16, 24, and 48 after irradiation, the spermatogenea index decreased from 3.4 to 1.5; 0.9 and 2.1, respectively, Erakond contributed to an increase in the spermatogenesis index to 1.9; 2.1 and 2.9 respectively.
In mice after irradiation, pre-implantation death at the stages of mature spermatozoa, spermatids, spermatocytes and spermatogonia increased from 24.1 to 41.0; 39.8 and 44.7; 42.0 %, respectively, and post-implantation death at the stages of mature sperm, spermatids, spermatocytes from 16.9; up to 26.5; 27.1 and 38.4 % respectively. The number of live fetuses per female decreased from 5.8 to 3.0; 3.9 and 3.7 respectively. Erakond statistically significantly contributed to the reduction of pre-implantation death at the stages of mature sperm, spermatids, spermatocytes and spermatogonia to 35.6; 33.4; 37.5 and 34.1, respectively, and a decrease in post-implantation death at the stages of mature sperm, spermatids and spermatocytes to 21.6; 20.5 and 28.2 respectively.
Conclusion: The data obtained indicate the possible preventive use of Erakond as an effective dietary supplement for the correction of spermatogenesis disorders when exposed to ionizing radiation.
Keywords: external acute γ-irradiation, spermatogenesis, spermatozoa, testis, Еracond, mice
For citation: Mamina VP. Radioprotective Effect of the Eracond on Spermatogenesis under the Influence of a Single External Acute γ-Irradiation. Medical Radiology and Radiation Safety. 2022;67(5):18–23. (In Russian). DOI: 10.33266/1024-6177-2022-67-5-18-23
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Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.06.2022. Accepted for publication: 25.08.2022.