Medical Radiology and Radiation Safety. 2022. Vol. 67. № 5
DOI: 10.33266/1024-6177-2022-67-5-41-46
E.A. Kodintseva1, 2, A.А. Akleyev3
EFFECT OF CHRONIC EXPOSURE AND NON-RADIATION FACTORS ON THE INTRACELLULAR STAT3 CONCENTRATION
1Urals Research Center for Radiation Medicine, Chelyabinsk, Russia
2Chelyabinsk State University, Chelyabinsk, Russia
3Southern-Urals State Medical University of the RF Ministry of Public Health, Chelyabinsk, Russia
Contact person: Е.А. Kodintseva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To evaluate the influence of radiation and non-radiation factors on the intracellular concentration of the transcription factor STAT3 in peripheral blood lymphocytes of chronically irradiated residents of the coastal villages of the Techa River in the long-term after the onset of irradiation.
Material and methods: The main group consisted of 50 people aged 67 to 84 years old with an average dose of irradiation of the red bone marrow of 727.9±79.1 mGy; thymus and peripheral lymphoid organs – 85.9±13.6 mGy. The comparison group included 25 people aged from 61 to 87 who were not exposed to accidents, whose distribution by sex and ethnicity corresponded to the composition of the main group. Peripheral blood lymphocyte lysates were normalized for total protein concentration prior to enzyme immunoassay.
Results: The median intracellular concentration of STAT3 was 167.6 (118.3–240.1) pg/ml in the main group and 147.0 (116.7–179.2) pg/ml in the control group.
Conclusion: The intracellular level of STAT3 did not differ significantly between chronically exposed and non-exposed individuals, as well as in people from different dose subgroups. Correlations between the concentration of STAT3 and dose characteristics, gender and ethnicity of the examined people were not found. In people with a red bone marrow dose of 0.85 Gy or more, STAT3 concentration correlated with age at the time of examination (SR= ‒0.67, p=0.01). In other dose subgroups, no correlation was found between the estimated indicator and the achieved age. The results are preliminary.
Keywords: chronic radiation exposure, the Techa River, transcription factor STAT3, intracellular concentration, peripheral blood lymphocytes
For citation: Kodintseva ЕА, Akleyev АА. Effect of Chronic Exposure and Non-Radiation Factors on the Intracellular STAT3 Concentration. Medical Radiology and Radiation Safety. 2022;67(5):41–46. (In Russian). DOI: 10.33266/1024-6177-2022-67-5-41-46
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.06.2022. Accepted for publication: 25.08.2022.