Medical Radiology and Radiation Safety. 2022. Vol. 67. № 6
DOI: 10.33266/1024-6177-2022-67-6-5-11
I.V. Kobzeva1, T.A. Astrelina1, V.A. Brunchukov1, V.A. Brumberg1, A.A. Rastorgueva1,
Yu.B. Suchkova1, D.Yu. Usupzhanova1, T.F. Malivanova1, V.A. Nikitina1, S.V. Lishchuk1,
E.A. Dubova1, K.A. Pavlov1, Ya.V. Tonkal1, O.F. Serova1,2, A.S. Samoilov1
Transplantation of Human Decellularized Amniotic Membrane in Local Radiation Injuries
1A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2Moscow Regional Perinatal Center, Balashikha, Russia
Contact person: T.A. Astrelina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Evaluation of the effectiveness of the use of human decellularized amniotic membrane, both as an independent covering material and as a cell-free matrix for syngeneic regenerative cells of adipose tissue (stromal-vascular fraction – SVF, adipose tissue ‒ AT) in local radiation injuries (LRI) IIIb-IV severity in laboratory animals.
Material and methods: 42 laboratory animals were studied. LRI modeling was carried out on an X-ray at a dose of 110 Gy. Animals were randomized randomly and divided into 6 groups depending on the type of treatment:
1st group (K) ‒ control group animals after irradiation did not receive specific treatment; the 2nd group (Gl) ‒ after irradiation, medical glue BF-6 was applied to the ulcer surface on the 21st day; 3rd group (Am) ‒ animals after irradiation on the ulcer surface was applied decellularized amniotic membrane, fixed with interrupted sutures on the 21st day; 4th group (Am-Gl) ‒ animals after irradiation on the ulcer surface was applied decellularized amniotic membrane, fixed with medical adhesive BF-6 for 21 days; 5th group (SVF-Gl) ‒ after irradiation, the SVF AT at a dose of 0.4×106 cells was applied to the ulcer surface after irradiation, followed by fixation with BF-6 medical glue for
21 days; the 6th group (Am-SVF) ‒ after irradiation, SVF AT was applied to the ulcer surface at a dose of 0.4×106 cells under the decellularized amniotic membrane, fixed with interrupted sutures on the 21st day.
Results: On the 112th day, complete healing of the ulcer was observed in 100 % of animals in the Am-Gl group, in 70 % in the Am and Gl groups. There was no complete healing of the ulcer in the SVF-CGl and Am-SVF groups. The greatest reduction in the area of the total altered skin from 21 to 112 days of the experiment was noted in the groups Gl-SVF (34.7 %), K (31.6 %), Am-SVF (30.7 %). In the Am-Gl and Am groups, a reduction in the area of the total altered skin was recorded by 24.6 % and 14.7 %, respectively. In the GL group, the reduction in the area of the total altered skin was the smallest, by only 13.5 %.
Conclusion: The use of a decellularized human amniotic membrane fixed with medical adhesive BF-6 can be considered as a promising method for the conservative treatment of LRI of the skin.
Keywords: local radiation injury, transplantation, decellularized human amniotic membrane, efficacy, rat
For citation: Kobzeva IV, Astrelina TA, Brunchukov VA, Brumberg VA, Rastorgueva AA, Suchkova YuB, Usupzhanova DYu, Malivanova TF, Nikitina VA, Lishchuk SV, Dubova EA, Pavlov KA, Tonkal YaV, Serova OF, Samoilov AS. Transplantation of Human Decellularized Amniotic Membrane in Local Radiation Injuries. Medical Radiology and Radiation Safety. 2022;67(6):5–11. (In Russian). DOI:10.33266/1024-6177-2022-67-6-5-11
References
1. Ilin L.A., Kirillov V.F., Korenkov I.P. Radiatsionnaya Gigiyena = Radiation Hygiene. A Textbook for Universities. Moscow, GEOTAR Media Publ., 2010 (In Russ.).
2. Fang Z., Chen P., Tang S., Chen A., Zhang C., Peng G., Li M., Chen X. Will Mesenchymal Stem Cells Be Future Directions for Treating Radiation-Induced Skin Injury? Stem. Cell. Res. Ther. 2021;12;12;1:179.
3. Higashi Y., Yusoff F.M., Kishimoto S., Maruhashi T. Regenerative Medicine for Radiation Emergencies. J. Radiat Res. 2021;5:62.
4. Vorobyeva N.Yu., Boyeva O.V., Osipov A.N., Bozhenko V.K. Radiation-Induced DNA Double-Strand Breaks and Dynamics of Apoptotic Death of Human Peripheral Blood Lymphocytes. Vestnik Rentgenologii i Radiologii = Journal of Radiology and Nuclear Medicine. 2008;4:6 (In Russ.).
5. Kim J.H., Jenrow K.A., Brown S.L. Mechanisms of Radiation-Induced Normal Tissue Toxicity and Implications for Future Clinical Trials. Radiat. Oncol. J. 2014;32;3:103-115.
6. Myung H., Jang H., Myung J.K., Lee C., Lee J., Kang J.H. Platelet-Rich Plasma Improves the Therapeutic Efficacy of Mesenchymal Stem Cells by Enhancing their Secretion of Angiogenic Factors in a Combined Radiation and Wound Injury Model. Exp. Dermatol. 2020;29:158-167.
7. Bray F.N., Simmons B.J., Wolfson A.H., Nouri K. Acute and Chronic Cutaneous Reactions to Ionizing Radiation Therapy. Dermatol. Ther. (Heidelb). 2016;6;2:185–206.
8. Ahmed E.A., Agay D., Schrock G., Drouet M., Meineke V., Scherthan H. Persistent DNA Damage after High Dose in Vivo Gamma Exposure of Minipig Skin. PLoS One. 2012;7;6:e39521.
9. Burnett L.R., Hughes R.T., Rejeski A.F., Moffatt L.T., Shupp J.W., Christy R.J., Winkfield K.M. Review of the Terminology Describing Ionizing Radiation-Induced Skin Injury: A Case for Standardization. Technol. Cancer. Res. Treat. 2021.
10. Yarnold J., Brotons M.C. Pathogenetic Mechanisms in Radiation Fibrosis. Radiother. Oncol. 2010;97;1:149-161.
11. Galstyan I.A., Nadezhina N.M. Local radiation injuries and their long-term consequences. Meditsina Truda i Promyshlennaya Ekologiya = Russian Journal of Occupational Health and Industrial Ecology. 2017;9:42-43
(In Russ.).
12. Brunchukov V., Astrelina T., Usupzhanova D., Rastorgueva A., Kobzeva I., Nikitina V., Lishchuk S., Dubova E., Pavlov K., Brumberg V. et al. Evaluation of the Effectiveness of Mesenchymal Stem Cells of the Placenta and Their Conditioned Medium in Local Radiation Injuries. Cells. 2020;29;9;12:2558
13. Sun J., Zhang Y., Song X., Zhu J., Zhu Q. The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats. Cell Transplant. 2019;28;1:105-115.
14. Chu C., Gao Y., Lan X., Lin J., Thomas A.M., Li S. Stem-Cell Therapy as a Potential Strategy for Radiation-Induced Brain Injury. Stem Cell Rev Rep. 2020;16;4:639-649.
15. Vyas K.S., Saba E.S., Tran N. Regenerative Properties of Autologous Fat Grafting in a Complicated Radiation-Induced Wound. Wounds. 2021;33;2:E20-E23.
16. Павлова О. Н., Гуленко О. Н., Девяткин А.А. Methods of Treatment and Prevention of Pterygium. Vestnik Meditsinskogo Instituta «REAVIZ». Reabilitatsiya, Vrach i Zdorovye = Bulletin of the Medical Institute “REAVIZ” (Rehabilitation, Doctor and Health). 2019;3:39 (In Russ.).
17. Silini A.R., Cargnoni A., Magatti M., Pianta S., Parolini O. The Long Path of Human Placenta, and Its Derivatives, in Regenerative Medicine. Front Bioeng Biotechnol. 2015;19;3:162.
18. Anselmo D.S., McGuire J.B., Love E., Vlahovic T. Application of Viable Cryopreserved Human Placental Membrane Grafts in the Treatment of Wounds of Diverse Etiologies: a Case Series. Wounds. 2018;30;3:57‐61.
19. Castellanos G., Bernab‐Garcia A., Moraleda J.M., Nicolas F.J. Amniotic Membrane Application for the Healing of Chronic Wounds and Ulcers. Placenta. 2017;59:146‐153.
20. Dhall S., Sathyamoorthy M., Kuang J.Q., et al. Properties of Viable Lyopreserved Amnion Are Equivalent to Viable Cryopreserved Amnion with the Convenience of Ambient Storage. PLoS ONE. 2018;13;10:e0204060.
21. Regulski M.J., Danilkovitch A., Saunders M.C. Management of a Chronic Radiation Necrosis Wound with Lyopreserved Placental Membrane Containing Viable Cells. Clin. Case Rep. 2019;28;7;3:456-460.
22. Kotenko K.V., Moroz B.B., Nasonova T.A., et al. Experimental Model of Severe Local Radiation Skin Lesions after Exposure to X-Rays. Patologicheskaya Fiziologiya i Eksperimentalnaya Terapiya = Pathological Physiology and Experimental Therapy. 2013;3:121–123 (In Russ.).
23. Samoylov A.S., Brumberg V.A. Sposob Polucheniya Beskletochnogo Matriksa Amnioticheskoy Membrany dlya Posleduyushchey Rekonstruktsii Defektov Tkaney = A Method for Obtaining a Cell-Free Matrix of the Amniotic Membrane for the Subsequent Reconstruction of Tissue Defects. Patent 2 751 353 C1 Russian Federation, IPC A61K35/50 A61L27/38 C12N5/73, No. 2020124830; Publ. 07.13.2021, Bull. No. 2 (In Russ.).
24. Bose B. Burn Wound Dressing with Human Amniotic Membrane. Ann. R. Coll. Surg. Engl. 1979;61:444‐447
25. Cornwell K.G., Landsman A., James K.S. Extracellular Matrix Biomaterials for Soft Tissue Repair. Clin. Podiatr. Med. Surg. 2009;26:507‐523.
26. John T. Human Amniotic Membrane Transplantation: Past, Present, and Future. Ophthalmol Clin. North. Am. 2003;16:43‐65.
27. Quinby J.W., Hoover H.C., Scheflan M., et al. Clinical Trials of Amniotic Membranes in Burn Wound Care. Plast. Reconstr. Surg. 1982;70:711‐717.
28. Sawhney C. Amniotic Membrane as a Biological Dressing in the Management of Burns. Burns. 1989;15:339‐342.
29. Diyarbakırlıoğlu M., Bağhaki S., Ercan A., et al. Effect of Fresh Human Amniotic Membrane on Radiation-Induced Wounds in a Murine Experimental Model. Eur. J. Plast. Surg. 2018;41:279–284.
30. Fernandez D. Cryopreserved Amniotic Membrane and Umbilical Cord for a Radiation-Induced Wound with Exposed Dura: a Case Report. J. Wound Care. 2019;1;28:S4-S8.
31. Lobo Gajiwala A., Sharma V. Use of Irradiated Amnion as a Biological Dressing in the Treatment of Radiation Induced Ulcers. Cell. Tissue. Bank. 2003;4;2-4:147-150.
32. Murphy S.V., Skardal A., Nelson R.A., Sunnon K., Reid T., Clouse C., Kock N.D., Jackson J., Soker S., Atala A. Amnion Membrane Hydrogel and Amnion Membrane Powder Accelerate Wound Healing in a Full Thickness Porcine Skin Wound Model. Stem. Cells Transl. Med. 2020;9;1:80-92.
33. Kakabadze Z., Chakhunashvili D., Gogilashvili K., Ediberidze K., Chakhunashvili K., Kalandarishvili K., Karalashvili L. Bone Marrow Stem Cell and Decellularized Human Amniotic Membrane for the Treatment of Nonhealing Wound After Radiation Therapy. Exp Clin Transplant. 2019;17;Suppl 1:92-98.
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Поступила: 20.07.2022. Принята к публикации: 25.09.2022.