Medical Radiology and Radiation Safety. 2023. Vol. 68. № 5
DOI:10.33266/1024-6177-2023-68-5-5-10
S.A. Abdullaev1, 4, D.V. Saleeva1, M.V. Dushenko1, 2,
N.F. Raeva1, А.I. Abdullaeva1, G.D. Zasukhina1, 3, A.N. Osipov1, 2
Protective Properties of Compound Aicar in Vivo Exposed to Radiation
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
3 N.I. Vavilov Institute of General Genetics, Moscow, Russia
4 Institute of Theoretical and Experimental Biophysics, Pushchino, Russia
Contact person: S.A. Abdullaev, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To study the effect of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) on the survival rate of mice and proportion of polychromatophilic erythrocytes (PCE) in the bone marrow cells with micronuclei (MN), as well as post-irradiation urinary excretion of cell-free nuclear DNA (cf-nDNA) and mitochondrial DNA (cf-mtDNA) in rats.
Material and methods: Male Balb/c mice aged 2 months and Fisher-344 male rats aged 3 months were used. To determine the survival rate of mice, X-irradiation was performed at a dose of 8 Gy, and to analysis the proportion of PCE in the bone marrow cells with MN, at a dose of 2 Gy. Rats were X-irradiated at a dose of 5 Gy. AICAR was administered to animals intraperitoneally at a dose of 400 mg/kg. The drug was administered 30 min before and 20 min after irradiation of the animals. The DNA content was measured by real-time PCR.
Results: The results of the study showed that the introduction of AICAR causes a statistically significant increase in the survival rate of irradiated animals. The greatest effect was shown in the group of mice treated with AICAR 20 min after their irradiation at a lethal dose. The introduction of AICAR before irradiation reduces the proportion of PCE with MN by 30 %, and after irradiation ‒ by 70 %, in comparison to the control. AICAR promoted enhanced urinary excretion of cf-nDNA and cf-mtDNA fragments in rats after irradiation.
Conclusion: The results show that AICAR acts as a radiomitigation effector and promotes active DNA excretion of damaged cell from animal tissues in the post-radiation period.
Keywords: X-rays, AICAR, survival rate, micronuclei, cell-free DNA in the urine, rats, mice
For citation: Abdullaev SA, Saleeva DV, Dushenko MV, Raeva NF, Abdullaeva АI, Zasukhina GD, Osipov AN. Protective Properties of Compound Aicar in Vivo Exposed to Radiation. Medical Radiology and Radiation Safety. 2023;68(5):5–10. (In Russian). DOI:10.33266/1024-6177-2023-68-5-5-10
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out on the topic of the A.I. Burnazyan Federal State Budgetary Research Center «Technology-3» (state task
No. 123011300105-3).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.04.2023. Accepted for publication: 27.05.2023.