Medical Radiology and Radiation Safety. 2024. Vol. 69. № 6
DOI:10.33266/1024-6177-2024-69-6-38-41
L.P. Sycheva1, A.F. Bobrov1, S.M. Kiselev1, T.M. Novikova2
Analysis of the Relationship of Biomarkers of Cytogenetic and Psychophysiological Status of Personnel under Conditions of the Combined Influence of Occupational Factors
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 Central Medical and Sanitary Unit No. 91, Sverdlovsk region, Lesnoy
Contact person: L.P. Sycheva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Background: Analysis of the Relationship of biomarkers of Cytogenetic and Psychophysiological Status (CGS and PPS) of personnel under conditions of the combined influence of occupational factors.
Material and methods: CGS was determined using a non-invasive Buccal Micronucleus Cytome Assay (BMCA) and determination of the accumulation index of cytogenetic damage and the level of cytogenetic stress. PPS of personnel was determined in accordance with
MR 2.2.9.84 – 2015 FMBA of Russia and the Health-Test program.
Results: People with average and high levels of psychophysiological adaptation have a low level of cytogenetic damages. Also, in the group with a high accumulation index of cytogenetic damages a low level of psychophysiological adaptation was determined.
Conclusion: An increased level of cytogenetic damages has been established in people with a low level of psychophysiological adaptation. Taking into account the literature data, we can assume an indirect dependence of the cytogenetic status of the organism on its psychophysiological adaptation, which may be due to hormonal imbalance and the induction of oxidative stress.
Keywords: personnel, ionizing radiation, chemical, cytogenetic status, psychophysiological status, buccal micronucleus cytome assay, micronucleus, apoptosis
For citation: Sycheva LP, Bobrov AF, Kiselev SM, Novikova TM. Analysis of the Relationship of Biomarkers of Cytogenetic and Psychophysiological Status of Personnel under Conditions of the Combined Influence of Occupational Factors. Medical Radiology and Radiation Safety. 2024;69(6):38–41. (In Russian). DOI:10.33266/1024-6177-2024-69-6-38-41
References
1. Holland N., Bolognesi C., Kirsch-Volders M., Bonassi S., Zeiger E., Knasmueller S., Fenech M. The Micronucleus Assay in Human Buccal Cells as a Tool for Biomonitoring DNA Damage: the HUMN Project Perspective on Current Status and Knowledge Gaps. Mutat. Res. 2008;659:93–108.
2. Fenech M., Holland N., Zeiger E., Chang W.P., Burgaz S., Thomas P., Bolognesi C., Knasmueller S., Kirsch-Volders M., Bonassi S. The HUMN and HUMNxL International Collaboration Projects on Human Micronucleus Assays in Lymphocytes and Buccal Cells - Past, Present and Future. Mutagenesis. 2011;26:239–245.
3. Organization and Conduct of Psychophysiological Examinations of Employees of Organizations Operating Especially Radiation-Hazardous and Nuclear-Hazardous Production Facilities and Facilities in the Field of Nuclear Energy Use, when Employees Undergo Medical Examinations in Medical Organizations of the Federal Medical and Biological Agency of Russia. 2.2.9.84 – 2015. (https://drive.google.com/file/d/0B_ WTIHndwyxNLXp4QTM1SkY3eVE/view?resourcekey=0-dpURYaUj69mF-HkAxbfTpQ) (In Russ.).
4. Bobrov A.F., Ivanov V.V., Kalinina M.Yu., Novikova T.M., Rataeva V.V., Sedin V.I., et al. Innovative Technology of Pre-Shift Psychophysiological Examination of Personnel as a Means of Increasing Radiation Exposure Safety. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost’ = Medical Radiology and Radiation Safety. 2018;5:5–10 (In Russ.).
5. HealthTest Program. Manual. Version: 10.2.1.26. Multidisciplinary Enterprise. St. Petersburg, ELSIS Publ. (In Russ.).
6. Fenech M., Knasmueller S., Knudsen L.E., Kirsch-Volders M., Deo P., Franzke B., Stopper H., Andreassi M.G., Bolognesi C., Dhillon V.S., Laffon B., Wagner K.H., Bonassi S. Micronuclei and Disease, Special Issue: Aims, Scope, and Synthesis of Outcomes. Mutat Res Rev Mutat Res. 2021;788:108384. doi: 10.1016/j.mrrev.2021.108384.
7. Abilev S.K., Glazer V.M., Aslanyan M.M. Osnovy Mutageneza i Toksikologii = Fundamentals of Mutagenesis and Toxicology. St. Petersburg, Nestor-Istoria Publ., 2012. 148 p. (In Russ.).
8. Durnev A.D., Zhanataev A.K., Eremina Yu.A. Geneticheskaya Toksikologiya = Genetic Toxicology. Moscow, Mittel Press Publ., 2022. 286 p. (In Russ.)
9. Radiation safety standards (NRB-99/2009). Sanitary and Epidemiological Rules and Regulations. Moscow, Federal Center for Hygiene and Epidemiology of Rospotrebnadzor Publ., 2009. 100 p. (In Russ.).
10. Sycheva L.P. Cytogenetic Monitoring to Assess the Safety of the Human Environment. Gigiyena i Sanitariya = Hygiene and Sanitation. 2012;91;6:68-72 (In Russ.).
11. Minkin V.A. Vibraimage, Cybernetics, Emotions. St. Petersburg, Renome Publ., 2020. 164 p. (In Russ.).
12. Gidron Y., Russ K., Tissarchondou H., Warner J. The Relation Between Psychological Factors and DNA-Damage: a Critical Review. Biological Psychology. 2006;72;3:291–304.
13. Bagchi D., Carryl O.R., Tran M.X., Bagchi M., Garg A., Milnes M.M., Williams C.B., Balmoori J., Bagchi D.J., Mitra S., Stohs S.J. Acute and Chronic Stress-Induced Oxidative Gastrointestinal Mucosal Injury in Rats and Protection by Bismuth Subsalicylate. Mol Cell Biochem. 1999;196;1-2:109-116.
14. Lesgards J.F., Durand P., Lassarre M., Stocker P., Lesgards G., Lanteaume A., Prost M., Lehucher-Michel M.P. Assessment of Lifestyle Effects on the Overall Antioxidant Capacity of Healthy Subjects. Environ Health Perspect. 2002;110;5:479-486.
15. Kang D.H. Oxidative Stress, DNA Damage, and Breast Cancer. AACN Clin Issues. 2002;13;4:540-549.
16. Hoeijmakers J.H. Genome Maintenance Mechanisms for Preventing Cancer. Nature. 2001;411;6835:366-374.
17. Bonassi S., El-Zein R., Bolognesi C., Fenech M. Micronuclei Frequency in Peripheral Blood Lymphocytes and Cancer Risk: Evidence from Human Studies. Mutagenesis. 2011;26;1:93-100.
18. Reimann H., Stopper H., Polak T., Lauer M., Herrmann M.J., Deckert J., Hintzsche H. Micronucleus Frequency in Buccal Mucosa Cells of Patients with Neurodegenerative Diseases. Sci Rep. 2020;10;1:22196.
19. Bolognesi C., Bonassi S., Knasmueller S, Fenech M., Bruzzone M., Lando C., Ceppi M. Clinical Application of Micronucleus Test in Exfoliated Buccal Cells: a Systematic Review and Metanalysis. Mutat Res Rev Mutat Res. 2015;766:20-31.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Sycheva L.P.: research design development, research, collection and analysis of literary material, statistical data processing, writing and scientific text editing. Bobrov A.F.: development of the research concept, development of research design, conducting research, scientific text editing. Kiselev S.M.: development of the research concept, development of research design, conducting research, writing and scientific editing of the text.Novikova T.M.: conducting research, scientific text editing.
Article received: 20.07.2024. Accepted for publication: 25.09.2024.