Medical Radiology and Radiation Safety. 2025. Vol. 70. № 2
DOI:10.33266/1024-6177-2025-70-2-35-39
M.V. Pustovalova1, V.D. Nekrasov1, E.V. Andreev3, 4, I.N. Fadeikina3, 4,
S.V. Leonov1, A.N. Nechaev3, 4, A.N. Osipov1
Synthesized Using β-Cyclodextrin Silver and Gold Nanoparticles
as Radiosensitizers in Breast Cancer Radiotherapy
1 Moscow Institute of Physics and Technology, Dolgoprudny, Russia
2 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
3 Joint Institute for Nuclear Research, Dubna, Russia
4 Dubna State University, Dubna, Russia
Contact person: A.N. Osipov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: 1) Synthesis and characterization of β-cyclodextrin stabilized silver and gold nanoparticles. 2) Evaluation of the effect of the synthesized nanoparticles on the severity of radiobiological effects in irradiated breast cancer (BC) cells.
Material and methods: Gold and silver nanoparticles were synthesized using β-cyclodextrin as a reducing agent and stabilizer. Human BC cell lines MDA-MB-231 (ER-/PR-/ EGFR+/ HER2-) and MCF7 (ER+/ PR+/ EGFR-/ HER2-) were used in the work. The cells were irradiated on a RUB RUST-M1 (Russia) X-ray irradiation facility, equipped with two X-ray emitters, at a dose rate of 0.85 mGy/min, 200 kV voltage, 5.0 mA current, 1.5 mm Al filter. Nanoparticles were added 24 h before irradiation at a concentration of 0.5 mg/l. Cells without nanoparticles were used as a control. To assess radiobiological effects, foci of the DNA damage marker protein (γH2AX) were analyzed 1 and 24 hours after irradiation. Statistical and mathematical data analysis was performed using GraphPad Prism 9.0.2.161 software (GraphPad Software). Statistical significance was assessed using analysis of variance (ANOVA).
Results: Effective radiosensitization of BC cell lines MDA-MB-231 and MCF7 using β-cyclodextrin stabilized gold and silver nanoparticles (AuNPs and AgNPs) was shown. The results obtained indicate the achievement of statistically significant results already at a concentration of 0.5 mg/l, which is at least 20 times less than the concentrations previously used to achieve a significant effect.
Keywords: breast cancer, radiation therapy, radiosensitizers, silver nanoparticles, gold nanoparticles, β-cyclodextrin
For citation: Pustovalova MV, Nekrasov VD, Andreev EV, Fadeikina IN, Leonov SV, Nechaev AN, Osipov AN. Synthesized Using β-Cyclodextrin Silver and Gold Nanoparticles as Radiosensitizers in Breast Cancer Radiotherapy. Medical Radiology and Radiation Safety. 2025;70(2):35–39. (In Russian). DOI:10.33266/1024-6177-2025-70-2-35-39
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. Radiobiological research was carried out with the support of the Russian Science Foundation (project No. 24-45-20002, https://rscf.ru/project/24-45-20002 /). The synthesis and characterization of nanoparticles were performed within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (No. 1024011000011-7-1.4.2;3.5.2 Conjugates of boron-containing quantum dots with biovectors for the diagnosis and boron-neutron capture therapy of superficial malignant tumors (FEEM-2024-0011)).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.12.2024. Accepted for publication: 25.01.2025.