Medical Radiology and Radiation Safety. 2017. Vol. 62. No. 4. P. 5-11

RADIATION BIOLOGY

DOI: 10.12737/article_59b10253ece3a0.60813066

Bioeffect of Negative Ionized Air Impact on the Course of Reparative Processes in the Organism after Gamma-Irradiation

S.V. Tatarkin¹, A.V. Shafirkin², M.Yu. Barantseva², S.M. Ivanova², S.V. Vorozhtsova², L.N. Tatarkina², A.Ch. Hurshut³, V.N. Bezgreshnov³

1. International Sustainable Energy Centre under the auspices of UNESCO, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Institution of Medicine Biology Problems, Moscow, Russia; 3. LLC Scientific-Industrial Center «Istion – Zdorov’ye», Moscow, Russia

S.V. Tatarkin – PhD Biol., Head of sector; A.V. Shafirkin – Dr. Sc. Biol., Head of Lab., M.Iu. Barantseva – PhD Biol., Leading Researcher; S.M. Ivanova – PhD Biol., Leading Researcher; S.V. Vorozhtsova – PhD Biol., Leading Researcher; L.N. Tatarkina – Research Worker; A.Ch. Hurshut – Deputy Chief Designer; V.N. Bezgreshnov – Chief Designer

Abstract

Purpose: The integrated research of the reaction of the hematopoietic system, including hematopoietic, metabolic changes in the cells and modify their antioxidant capacity during the recovery period after radiation exposure at doses and concentrations that are typical for interplanetary flight: 1) in isolation, i.e. without any external influence; 2) under the influence of negatively ionized air.

Material and methods: The study was conducted on sexually mature mice of line F1(CBA×C57BL6) subject to the rules and regulations of biomedical ethics on the basis of SSC RF MPIB RAS. The pilot study included 2 stages: 1) fractionated γ-irradiation (350 cGy) with the duration of 63 days; 2) the recovery period, lasting 28 days, during which the animals were exposed to negatively ionized air (daily for 70 min). Studied cytological, morphological, biochemical parameters of hematopoiesis: the total number of nucleated cells (bone marrow); the amount of production of erythrocytes, reticulocytes, hemoglobin (in peripheral blood); the content of ATP, lactate, glutathione, activity of glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH) (in erythrocytes); the ratio and amount of lipid fractions (in the membrane of erythrocytes).

Results: The biological effect of the impact of negatively ionized air on the course of reparative processes in the body after gamma irradiation consists in the accelerated recovery of the number of peripheral blood cells and in a more pronounced degree of myeloid growth, due to:

• increase in the rate of recovery of energy production processes (increase in LDH activity, improvement of lactate and ATP, and more pronounced normalization of oxidation-reduction processes), which facilitates recapitalization of cells with lipid membrane saturation with membrane cholesterol;
• membrane-protective effect of negatively ionized air, based on the restoration of the membrane potential of cells expressing negative effects of blood cells;
• increased life span of cells in peripheral blood and smaller values of the half-life of granulocytes from the bloodstream in the tissue.

Key words: hemopoesis, reparative processes, gamma-irradiation, negative ionized air (air ions), cellular metabolism, membrane potential

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For citation: Tatarkin SV, Shafirkin AV, Barantseva MYu, Ivanova SM, Vorozhtsova SV, Tatarkina LN, Hurshut ACh, Bezgreshnov VN. Bioeffect of Negative Ionized Air Impact on the Course of Reparative Processes in the Organism after Gamma-Irradiation. Medical Radiology and Radiation Safety. 2017;62(4):5-11. Russian. DOI: 10.12737/article_59b10253ece3a0.60813066

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