Medical Radiology and Radiation Safety. 2017. Vol. 62. No. 5. P. 5-10

RADIATION BIOLOGY

DOI: 10.12737/article_59f2ef130f5421.00591025

Study of Uridylic Nucleotides Contents and the Investigation Aspartate Carbamoyltransferase in Liver and Small Intestine Mucosa Exposed when Administered to Rats Orotic Acid and Perftoran

E.R. Nagiev, S.E. Nagieva, F.E. Ismailova

Dagestan State Medical University, Makhachka, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

E.R. Nagiev – Academician of the Russian Academy of Natural Sciences, Honored Worker of the Higher School of Russian Federation, Prof., Dr. Sc. Med., Head the Department of General and Biological Chemistry, Dagestan State Medical University; S.E. Nagieva – PhD Med., Assoc. Prof.; F.E. Ismailova – PhD Med., Assistant

Abstract

Purpose: Study of uridylic nucleotides content and aspartate carbamoyltransferase which was a key enzyme on the pathway for the synthesis of pyrimidine nucleotides in tissues of irradiated animals upon administration of orotic acid and perftorane was conducted.

Material and methods: Studies were performed on random-bred albino rats subjected to a single γ-ray exposure at a total dose of 6 Gy. Orotic acid was injected as potassium salt in a dose of 60 mg / kg, perftoran salt in a dose of 1 ml / 100 g water.

Results: A decrease in the content of UTP and UDP, as well as an increase in UMP after irradiation, especially on the 7^th day, was established. The most pronounced changes in the studied biochemical parameters take place in the mucosa of the small intestine. The administration of orotic acid and perftorane to irradiated animals contributes to a significant correction of both the nucleotide content and the activity of aspartate carbamoyltransferase.

Conclusion: The radiation leads to some decrease in the content of UDP and UTP. Changes in the content of nucleotides and activity of aspartate carbamoyltransferase in the mucosa of the small intestine are more pronounced in comparison with liver tissue. The combined administration of orotic acid and perftorane promotes the normalization of the content of nucleotides, and the activity of aspartate carbamoyltransferase in the liver and mucous membrane of the small intestine of irradiated animals.

Key words: uridylic nucleotides, aspartate carbamoyltransferase, irradiation, orotic acid, perftoran, liver, small intestine, mucosa

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For citation: Nagiev ER, Nagieva SE, Ismailova FE. Study of Uridylic Nucleotides Contents and the Investigation Aspartate Carbamoyltransferase in Liver and Small Intestine Mucosa Exposed when Administered to Rats Orotic Acid and Perftoran. Medical Radiology and Radiation Safety. 2017;62(5):5-10. Russian. DOI: 10.12737/article_59f2ef130f5421.00591025

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