Medical Radiology and Radiation Safety. 2019. Vol. 64. No. 1. P. 15–20


DOI: 10.12737/article_5c55fb18bed8d6.85887139

I.K. Belyaev

Protection of Spermatogenesis with β-Carothin in Radiation Exposure.
Message 2: Carotenoid Enrichment of Diet with Single Acute External γ-Irradiation

A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

I.K. Belyaev – Head of Lab., Senior Researcher, PhD Biol.


Purpose: Experimental evaluation of the efficiency of β-carotene by single acute γ-irradiation of spermatogenesis correction radiation-induced disorders in males (♂) of Wistar rats during prolonged carotenoid enrichment of a diet.

Material and methods: Single external gamma irradiation ♂ was performed at the IGUR facility (137Cs, dose rate 0.029 Gy/s). The carotenoid used up on feeding ♂ in a mixture with cottage cheese for 2 months before irradiation, then – throughout the study period three times a week. During the days that ♂ were in cells with intact ♀ β-carotene was administered ♂ orally by a probe in the form of a suspension in vegetable oil. The damage and effectiveness of protection of spermatogenesis of irradiated ♂ were judged by the state of their reproductive function.

Results: β-carotene in intact ♂ rats reduced the overall, pre- and postimplantation embryonic mortality, respectively, from 29 to 14, from 22 to 9, and from 10 to 5 %. At the stage of mature sperm following irradiation ♂ dose of 3 Gy – total and pre-implantation mortality from 45 to 29, and from 23 to 9 %. After irradiation at a dose of 5 Gy – total, an pre- and postimplantation induced embryonic death, respectively, from 67 to 56, from 23 to 13 % and from 56 to 43 %. At the stage of spermatids after irradiation in dose 1 Gy – pre-implantation mortality from 23 to 9 %; in the dose of 3 Gy – the number of dead embryos in pregnant ♀ from 12 to 3; at a dose of 5 Gy total embryonic mortality from 57 to 49 %. Carotenoid enrichment of a diet of ♂ irradiated at doses of 3 and 5 Gy, has not corrective impact on the state indicators of their reproductive function, evaluated at fertilization of ♀ sperm irradiated at the stage of spermatocyte. At the stage of spermatogonia after irradiation at a dose of 3 Gy β-carotene increased fertility of ♂ 7 to 12 living embryos and reduced the overall, before – induced postimplantation mortality, respectively, with 63 and 43, from 42 to 25 and from 35 to 19 %. No statistically significant effect of β-carotene on the weight loss of the gonads irradiated ♂ was revealed. Compared to short-term regimen, long-term enrichment of a diet of irradiated ♂ β-carotene more effectively reduced embryonic mortality of their offspring.

Conclusions: Correction effects of β-carotene enrichment of the diet in radiation-induced disturbances by single acute external γ-irradiation at doses of 3 and 5 Gy of spermatogenesis in Wistar rats at stages of mature spermatozoa, spermatids and spermatogonia have been established.

Key words: external acute γ-irradiation, spermatogenesis, β-carotene, introduction to the diet, rats


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3. Vakulova LA, Zhidkova TA, Samokhvalov GI, Khristoforov VL. Method for obtaining beta-carotene. 1995. Patent number 2032667. (Russian).

For citation: Belyaev IK. Protection of Spermatogenesis with β-Carothin in Radiation Exposure. Message 2: Carotenoid Enrichment of Diet with Single Acute External γ-Irradiation. Medical Radiology and Radiation Safety. 2019;64(1):15-20. (Russian).

DOI: 10.12737/article_5c55fb18bed8d6.85887139

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