JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 3
DOI:10.33266/1024-6177-2024-69-3-13-18
A.A. Osipov1, A.K. Chigasova1, 2, 3, E.I. Yashkina1, 2, M.A. Ignatov1, 2,
N.Yu. Vorobyеva1, 2, A.N. Osipov1, 2
Link Between Cellular Senescence and Changes in The Number and Size of Phosphorylated Histone H2ax Foci in Irradiated Human Fibroblasts
1 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
2 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
3 Institute of Biochemical Physics, Moscow, Russia
Contact person: N.Yu. Vorobyеva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To analyze the relationship between cellular aging and changes in the number and size of phosphorylated histone H2AX (γH2AX) foci in human fibroblasts and their descendants (up to 15 passages) after exposure to low and high doses of X-ray radiation.
Material and methods: The work was performed on a culture of human skin fibroblasts. Cells were irradiated in the exponential growth phase on an X-ray biological unit RUB RUST-M1 (Russia), equipped with two X-ray emitters, at a dose rate of 40 mGy/min (dose 100 mGy) or 850 mGy/min (doses 2000 and 5000 mGy) and temperature 4˚C. Immunocytochemical staining was used to assess the number and size of γH2AX foci and the proportion of proliferating cells using antibodies to γH2AX and Ki67 (a cell proliferation marker protein), respectively. To assess cellular senescence, the proportion of cells positive for senescence-associated β-galactosidase (CA-β-gal(+)) was analyzed. Statistical and mathematical analysis of the obtained data was carried out using the statistical software package Statistica 8.0 (StatSoft).
Results: The studies showed that irradiation of cultured human fibroblasts at a low dose (100 mGy) does not lead to statistically significant changes in the number and size of γH2AX foci, as well as the proportion of non-proliferating and senescent cells in the progenies of irradiated cells up to the 15th passage after irradiation. The phenomenon of aging-associated persistence of an increased number and size of γH2AX foci in passages of cells irradiated at a dose of 5000 mGy was discovered. Mathematical analysis of the relationship between changes in the proportion of CA-β-gal(+) cells, the number and size of γH2AX foci in populations of irradiated cells indicates that radiation-induced cellular aging is more associated with the size, rather than the number, of γH2AX foci.
Keywords: fibroblasts, X-ray radiation, γH2AX, senescence, proliferation, late radiation-induced effects
For citation: Osipov AA, Chigasova AK, Yashkina EI, Ignatov MA, Vorobyеva NYu, Osipov AN. Link Between Cellular Senescence and Changes in The Number and Size of Phosphorylated Histone H2ax Foci in Irradiated Human Fibroblasts. Medical Radiology and Radiation Safety. 2024;69(3):13–18. (In Russian). DOI:10.33266/1024-6177-2024-69-3-13-18
References
1. Dabin J, Mori M, Polo SE. The DNA Damage Response in the Chromatin Context: a Coordinated Process. Curr Opin Cell Biol. 2023;82:102176. doi: 10.1016/j.ceb.2023.102176.
2. Georgoulis A, Vorgias C, Chrousos G, Rogakou E. Genome Instability and γH2AX. International Journal of Molecular Sciences. 2017;18(9). doi: 10.3390/ijms18091979.
3. Shibata A, Jeggo PA. DNA Double-Strand Break Repair in a Cellular Context. Clin Oncol (R Coll Radiol). 2014;26(5):243-9. doi: 10.1016/j.clon.2014.02.004.
4. Rothkamm K, Barnard S, Moquet J, Ellender M, Rana Z, Burdak-Rothkamm S. DNA Damage Foci: Meaning and Significance. Environ Mol Mutagen. 2015;56(6):491-504. doi: 10.1002/em.21944.
5. Biswas H, Makinwa Y, Zou Y. Novel Cellular Functions of ATR for Therapeutic Targeting: Embryogenesis to Tumorigenesis. International Journal of Molecular Sciences. 2023;24(14). doi: 10.3390/ijms241411684.
6. Bushmanov A, Vorobyeva N, Molodtsova D, Osipov AN. Utilization of DNA Double-Strand Breaks for Biodosimetry of Ionizing Radiation Exposure. Environmental Advances. 2022;8. doi: 10.1016/j.envadv.2022.100207.
7. Merighi A, Gionchiglia N, Granato A, Lossi L. The Phosphorylated Form of the Histone H2AX (γH2AX) in the Brain from Embryonic Life to Old Age. Molecules. 2021;26(23). doi: 10.3390/molecules26237198.
8. Palla V-V, Karaolanis G, Katafigiotis I, Anastasiou I, Patapis P, Dimitroulis D, et al. gamma-H2AX: Can It Be Established as a Classical Cancer Prognostic Factor? Tumor Biology. 2017;39(3). doi: 10.1177/1010428317695931.
9. Marcotte R, Lacelle C, Wang E. Senescent Fibroblasts Resist Apoptosis by Downregulating Caspase-3. Mech Ageing Dev. 2004;125(10-11):777-83. doi: 10.1016/j.mad.2004.07.007.
10. Neumaier T, Swenson J, Pham C, Polyzos A, Lo AT, Yang P, et al. Evidence for Formation of DNA Repair Centers and Dose-Response Nonlinearity in Human Cells. Proceedings of the National Academy of Sciences. 2011;109(2):443-8. doi: 10.1073/pnas.1117849108.
11. Belov O, Chigasova A, Pustovalova M, Osipov A, Eremin P, Vorobyeva N, et al. Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation. Current Issues in Molecular Biology. 2023;45(9):7352-73. doi: 10.3390/cimb45090465.
12. Vaurijoux A, Voisin P, Freneau A, Barquinero JF, Gruel G. Transmission of Persistent Ionizing Radiation-Induced Foci Through Cell Division in Human Primary Cells. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2017;797-799:15-25. doi: 10.1016/j.mrfmmm.2017.03.003.
13. Stewart RD. Two-Lesion Kinetic Model of Double-Strand Break Rejoining and Cell Killing. Radiation Research. 2001;156(4):365-78. doi: 10.1667/0033-7587(2001)156[0365:tlkmod]2.0.co;2.
14. Miller I, Min M, Yang C, Tian C, Gookin S, Carter D, et al. Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence. Cell Rep. 2018;24(5):1105-12 e5. doi: 10.1016/j.celrep.2018.06.110.
15. Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Lleres D, et al. The cell proliferation antigen Ki-67 organises heterochromatin. Elife. 2016;5:e13722. doi: 10.7554/eLife.13722.
16. Sobecki M, Mrouj K, Colinge J, Gerbe F, Jay P, Krasinska L, et al. Cell-Cycle Regulation Accounts for Variability in Ki-67 Expression Levels. Cancer Res. 2017;77(10):2722-34. doi: 10.1158/0008-5472.CAN-16-0707.
17. Maier AB, Westendorp RG, D VANH. Beta-Galactosidase Activity as a Biomarker of Replicative Senescence during the Course of Human Fibroblast Cultures. Annals of the New York Academy of Sciences. 2007;1100:323-32. doi: 10.1196/annals.1395.035.
18. Osipov A, Chigasova A, Yashkina E, Ignatov M, Fedotov Y, Molodtsova D, et al. Residual Foci of DNA Damage Response Proteins in Relation to Cellular Senescence and Autophagy in X-Ray Irradiated Fibroblasts. Cells. 2023;12(8). doi: 10.3390/cells12081209.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out with the support of the RSF (project No. 23-14-00078).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.01.2024. Accepted for publication: 27.02.2024.