JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
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Medical Radiology and Radiation Safety. 2023. Vol. 68. № 6
DOI:10.33266/1024-6177-2023-68-6-55-59
E.A. Kodintseva1, 2, A.А. Akleyev3
Effect of Radiation and Non-Radiation Factors on the ТBX21 Concentration in lysates of the Mitogen-Stimulated Mononuclear Cells
1 Urals Research Center for Radiation Medicine, Chelyabinsk, Russia
2 Chelyabinsk State University, Chelyabinsk, Russia
3 South-Ural State Medical University, Chelyabinsk, Russia
Contact person: Екатерина Александровна Кодинцева, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To evaluate the effect of radiation and non-radiation factors on the intracellular concentration of the ТBX21 transcription factor in peripheral blood PHA-stimulated mononuclear cells of chronically exposed residents of the Techa Riverside settlements in the long-term period after the start of exposure.
Material and methods: The main group consisted of 30 people aged 67–80 years with the mean dose to the red bone marrow 867±136 mGy, to thymus and peripheral lymphoid organs 125±20 mGy. The comparison group included 10 unexposed people aged 63–82 years. The main and the comparison groups had similar sex and ethnic composition. Peripheral blood mononuclear cells were PHA-stimulated for 24 hours. Cellular lysates were normalized by total protein concentration prior to being used for ELISA test.
Results: After 24 hours of incubation the median and interquartile range of the intracellular TBX21 concentration in mononuclear cells was 34.2 (6.6–86.0) pg/ml after the mitogen stimulation, and 0 (0–24.9) pg/ml – without mitogen stimulation (р=0.001). In the comparison group these values made up 24.8 (0.2–47.6) and 13.0 (0–19.2) pg/ml, respectively
Conclusion: The intracellular TBX21 concentration after 24-hour mitogen stimulation did not differ statistically significantly in chronically exposed and unexposed people, as well as in people from different dose groups. Statistically significant increase in TBX21 concentration in the lysates of mononuclear cells that were PHA-stimulated for 24 hours relative to non-mitogen stimulated cells was noted in chronically exposed people with medium and high doses to the red bone marrow. No correlation was revealed between the TBX21 concentration and dose characteristics, sex, and ethnicity of the studied individuals. The results are preliminary.
Keywords: chronic radiation exposure, residents, the Techa River, peripheral blood, ТBX21 transcription factor, lysates of mononuclear cells, Phytohemagglutinin
For citation: Kodintseva ЕА, Akleyev АА. Effect of Radiation and Non-Radiation Factors on the ТBX21 Concentration in lysates of the Mitogen-Stimulated Mononuclear Cells. Medical Radiology and Radiation Safety. 2023;68(6):55–59. (In Russian). DOI:10.33266/1024-6177-2023-68-6-55-59
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. This study was carried out within the framework of the state assignment of the FMBA of Russia: “The state of human cellular immunity in the period of the development of the late effects of chronic radiation exposure”.
Contribution. All authors confirm that their authorship meets the international ICMJE criteria. Kodintseva Е.А. — participated in the development of the concept and design of the study, research, data analysis and interpretation, preparation of the article; read and approved the final version before publication. Akleуev А.А. — participated in the development of the concept and design of the study, interpretation of results, scientific editing; read and approved the final version before publication
Article received: 20.07.2023. Accepted for publication: 27.08.2023.