JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2023. Vol. 68. № 6
DOI:10.33266/1024-6177-2023-68-6-92-98
T.F. Malivanova, T.A. Astrelina, I.V. Kobzeva, V.A. Nikitina, Y.B. Suchkova,
A.I. Golovkova, A.S. Ostashkin, D.Y. Usupzhanova, V.A. Brunchukov,
A.A. Rastorgueva, E.I. Dobrovolskaya, A.P. Kirilchev, M.Yu. Sukhova,
O.G. Mikhadarkina, N.V. Sokolova, A.S. Samoilov
Features of the Systemic Response to Adjuvant Radiation Therapy
in Carriers of Polymorphism -308(G/A)TNF in Breast Cancer Patients
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Tatyana Feodorovna Malivanova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Background: Adjuvant radiation therapy (ART), an integral part of locoregional breast cance (BC) therapy, acting not only locally, but also systemically and leads to a shift in homeostasis, which is reflected in routine general clinical tests. Tumor necrosis factor (TNF) is a proinflammatory cytokine, the production of which can be influenced by the single-nucleotide substitution -308(G/A)TNF. The minor allele -308A can be included in the stable inherited haplotype AH8.1 of the HLA gene complex. At the same time, the carriage of the -308A without the AH8.1 haplotype is associated with a poor prognosis in patients with BC. The aim of the study was to evaluate the features of the systemic response to the course of ART in carriers of TNF-associated genotypes with BC.
Material and methods: The sample is represented by 147 BC patients who underwent a course of ART (2 Gy in 25 fractions). Clinical and morphological characteristics and data of general clinical blood analysis were obtained from medical histories. Venous blood samples for the study were obtained at the beginning and at the end of the ART course. Alleles -308(G/A)TNF and marker alleles of haplotype AH8.1 (HLA-A×01, HLA-B×08 and HLA-DRB1×03) were determined by allele-specific PCR. sTNF concentrations were determined by the ELISA in 102 blood plasma samples.
Results: TNF-associated comparison groups were identified based on genotyping: (1) 114 carriers -308GG of the TNF gene, regardless of the AH8.1 haplotype (77,6 %); (2) 23 carrier -308A(AH8.1pos) had at least one AH8.1 marker allele (15.6 %); (3) 10 carriers -308A(AH8.1neg) did not have any AH8.1 marker allele (6.8 %). In the -308A(AH8.1neg) group the average concentration of sTNF both at the beginning and at the end of ART was significantly higher and, unlike other comparison groups, did not significantly decrease at the end of the ART course. A significant decrease in absolute values was revealed during ART in a number of cases for leukocytes, platelets and lymphocytes, however within the reference values. In the group -308A(AH8.1neg) correlation analysis revealed a high strength of positive connections between sTNF and leukocytes (r=0.71; p=0.027), platelets (r=0.67; p=0.04), neutrophils (r=0.70; p=0.027) only at the end of ART, whereas at the beginning ART these correlations were weak (r≤0.3) and statistically unreliable. For other genetic groups, the revealed correlations were not strong enough.
Conclusion: The revealed features of the systemic response to ART for carriers of a prognostically unfavorable genotype -308A(AH8.1neg) – a high concentration of sTNF and a positive correlation with the content of leukocytes (probably due to neutrophils) and platelets – can be considered as targets of individualized therapy.
Keywords: breast cancer, adjuvant radiation therapy, tumor necrosis factor, genomic polymorphism
For citation: Malivanova TF, Astrelina TA, Kobzeva IV, Nikitina VA, Suchkova YB, Golovkova AI, Ostashkin AS, Usupzhanova DY, Brunchukov VA, Rastorgueva AA, Dobrovolskaya EI, Kirilchev AP, Sukhova MYu, Mikhadarkina OG, Sokolova NV, Samoilov AS. Features of the Systemic Response to Adjuvant Radiation Therapy in Carriers of Polymorphism -308(G/A)TNF in Breast Cancer Patients. Medical Radiology and Radiation Safety. 2023;68(6):92–98. (In Russian). DOI:10.33266/1024-6177-2023-68-6-92-98
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study was carried out with the financial support of the Federal Target Program “Ensuring Nuclear and Radiation Safety for 2016-2020 and for the period up to 2035”.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.07.2023. Accepted for publication: 27.08.2023.