JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-15-20
S.M. Rodneva1, L.P. Sycheva1, A.A. Maksimov1, E.S. Zhorova1, A.A. Tsishnatti1,
G.S. Tishchenko1, Yu.A. Fedotov1, 2, T.M. Trubchenkova1, E.I. Yashkina1,
D.V. Guryev1, V.G. Barchukov1
Genotoxic Effects in Spleen and Bone Marrow of Wistar Rats Chronically Exposed to Tritium Oxide and 3H-Thymidine with Drinking Water
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
Contact person: Sofya Mikhailovna Rodneva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: The operation of nuclear facilities such as NPPs is accompanied by the formation and release of tritium compounds into the environment. Currently available data on the biological effects of tritium, especially its organic compounds, are very scattered and fragmentary. There is still no consensus on the regulation of the content of inorganic and organic tritium compounds in different environments. This leads, for example, to a wide range of permissible levels of tritium in the environment in different countries, which requires additional experimental and calculated data in order to harmonize standards and ensure the safety of the members of the public living near such nuclear facilities. The purpose of this work is a comparative assessment of molecular cellular biological effects (formation of DNA double-strand breaks and micronuclei) upon exposure to individual tritiated compounds in mammals.
Material and methods: An in vivo study was conducted on male rats that received orally drinking water containing tritium oxide (HTO) or 3H-thymidine with a volumetric specific activity of 800 kBq/l for 10, 21 and 31 days. The number of DNA double-strand break (DSB) repair foci in rat splenocytes was assessed by immunocytochemical staining of phosphorylated histone H2AX (γH2AX) foci. An analysis of the frequencies of polychromatophilic erythrocytes (PCE) with micronuclei (MN) in the bone marrow of rats was also carried out.
Results: The genotoxic effect of both forms of tritium was determined by a significant yield of DNA DSBs in splenocytes and micronuclei in bone marrow PCE (a more pronounced effect on the 31st day of 3H-thymidine action). With chronic exposure to 3H-thymidine on days 21 and 31, the number of γH2AX foci significantly increases; in the case of HTO, the level of foci on days 31 does not significantly change. Exposure to HTO and 3H-thymidine caused approximately the same induction of PCE with micronuclei on days 10 and 21, but by day 31 the effect of 3H-thymidine was approximately 40 % greater than that of HTO. The experiment revealed a likely genotoxic effect of inhaled tritium in control rats that were kept in the same room as the rats that received HTO and 3H-thymidine orally. However, additional experiments are needed to confirm this effect.
Conclusion: The result of this work expands the understanding of the mutation process in mammalian cells exposed to internal ionizing radiation when taking compounds containing tritium. Increased genotoxicity is observed when rats ingest drinking water containing tritium with an activity of 800 kBq/l.
Keywords: tritium, tritiated water, organically bound tritium, 3H-thymidine, γH2AX foci, DNA double-strand breaks, splenocytes, micronucleus test on polychromatophilic erythrocytes, rats
For citation: Rodneva SM, Sycheva LP, Maksimov AA, Zhorova ES, Tsishnatti AA, Tishchenko GS, Fedotov YuA, Trubchenkova TM, Yashkina EI, Guryev DV, Barchukov VG. Genotoxic Effects in Spleen and Bone Marrow of Wistar Rats Chronically Exposed to Tritium Oxide and 3H-Thymidine with Drinking Water. Medical Radiology and Radiation Safety. 2024;69(5):15–20. (In Russian). DOI:10.33266/1024-6177-2024-69-5-15-20
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The research was carried out within the framework of the state task, the research topic is «Track-1» reg. # AAAA-A19-119031190033-1.
Contribution. Rodneva S.M., Sycheva L.P., Maksimov A.A., Zhorova E.S., Guryev D.V. – preparation of the text of the article, analysis and interpretation of data, conducting experiments, collection and analysis of literary material; Zhorova E.S., Guryev D.V. – development of the concept and design of the study, internal audit; Rodneva S.M., Sycheva L.P., Maksimov A.A., Zhorova E.S., Tsishnatti A.A., Tishchenko G.S., Fedotov Yu.A., Trubchenkova T.M. a., Yashkina E.I., Guryev D.V. – conducting experiments and statistical data processing; Rodneva S.M., Sycheva L.P., Guryev D.V., Maksimov A.A., Barchukov V.G. – scientific editing of the text, verification of critically important intellectual content; Guryev D.V. – approval of the final version of the manuscript.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.