JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
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The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
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The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-21-27
L.A. Romodin, А.А. Moskovskij
Assessment of the Effect of Ascorbic, Malic and Succinic Acids
on Radiation-Induced Oxidative Stress in A549 Cells
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: L.A. Romodin, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Relevance: For modern radiobiology, the problem of finding pharmacological protection against radiation damage remains urgent. Interest in this topic does not weaken due to the high chemical toxicity of all generally recognized radioprotectors. One of the most studied drugs in this regard are substances with antioxidant activity, which is due to the ability of antioxidants to inhibit the processes of oxidative stress.
Purpose: The effect of malic, succinic and ascorbic acids on radiation-induced oxidative stress in the culture of human lung adenocarcinoma cells of the A549 line.
Material and methods: In the course of the work, the effect of solutions of malic, ascorbic and succinic acids in concentrations of 0.1, 0.5, 1 and 2 mM on the intensity of radiation-induced oxidant stress in the adsorption culture of cells of the A549 line was studied. Oxidative stress was induced by X-ray radiation at a dose of 8 Gy. The level of reactive oxygen species was estimated based on the ratio of the fluorescence intensity of the dye dichlorofluorescein to that of the dye Hoechst-33342.
Results: Under the influence of the studied substances, a statistically significant decrease in the content of reactive oxygen species in the cells was observed. The most pronounced effect is observed in samples treated with succinic acid. In non-irradiated samples in the presence of ascorbic and malic acids at a concentration of the studied substances of 100 mM, a statistically significant increase in the intensity of fluorescence is observed, which can be explained by the reduction of intracellular trivalent iron to Fe2+ under the action of these substances, which contributed to the Fenton reaction.
Conclusions: Based on the results obtained during this study, it can be assumed that malic acid, ascorbic acid and, in particular, succinic acid have some radioprotective properties. However, additional studies on other model systems, including various cell lines, are needed to confirm the presence of these properties. The results of the presented work make it possible in the future to begin the development of therapeutic schemes to alleviate the effects of radiation using the studied substances.
Keywords: A549 cells, X-rays, oxidative stress, malic acid, ascorbic acid, succinic acid
For citation: Romodin LA, Moskovskij АА. Assessment of the Effect of Ascorbic, Malic and Succinic Acids on Radiation-Induced Oxidative Stress in A549 Cells. Medical Radiology and Radiation Safety. 2024;69(5):21–27. (In Russian). DOI:10.33266/1024-6177-2024-69-5-21-27
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out within the framework of research and development «Technology-3» (the registration number of research and development in the EGISU R&D system: 1230113001053).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.