JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-59-65
A.I. Kotikova, V.S. Nikiforov, E.A. Blinova, A.V. Akleyev
Assessment of T-Regulatory Cell Population and Foxp3 Gene Expression in Chronically Exposed Residents of the Urals Region
Urals Research Center for Radiation Medicine, Chelyabinsk, Russia
Chelyabinsk State University, Chelyabinsk, Russia
Contact person: Alisa Igorevna Kotikova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: To conduct a pilot study on the quantity of regulatory T-cells (Treg) in the peripheral blood and to assess transcriptional activity of FOXP3 gene in chronically exposed persons.
Material and methods: The study included 77 participants who were divided into two groups: exposed people – 45 individuals, with an average accumulated dose to red bone marrow (RBM) of 641.21±80.41 mGy, and a comparison group – 32 individuals, with an average accumulated RBM dose of 20.38±2.51 mGy. The study on the assessment of FOXP3 gene expression was conducted on 298 individuals: the irradiated group consisted of 163 individuals with an average accumulated dose to RBM of 702±43.10 mGy; the comparison group included 135 individuals with an average accumulated dose to RBM of 17.30±1.40 mGy. The study groups did not differ significantly by age, sex and ethnicity. Quantitative assessment of regulatory T-cells in the peripheral blood was performed using flow cytometry method by the presence of T-helper markers CD3 and CD4, high expression of marker CD25 and low expression of marker CD127. Thus, the phenotype of T-regulatory lymphocytes was described as CD3+CD4+CD25highCD127low. The relative mRNA content of the FOXP3 gene was assessed by PCR-RT.
Results: More than 70 years after the onset of chronic radiation, no statistically significant changes in the pool of regulatory T-cells were detected in the exposed persons: the content of absolute and relative amount of Treg did not differ statistically significantly between the studied groups (p=0.91 and p=0.29, respectively); no statistically significant relationship of Treg indices with the accumulated doses to RBM and thymus and peripheral lymphoid organs were found. No statistically significant differences in FOXP3 gene mRNA expression were found between exposed individuals and the comparison group. A linear positive dependence of FOXP3 gene mRNA expression on the relative number of regulatory T-cells was shown (p=0.007).
Keywords: chronic exposure, regulatory T cells, immunity, gene expression, FOXP3
For citation: Kotikova AI, Nikiforov VS, Blinova EA, Akleyev AV. Assessment of T-Regulatory Cell Population and Foxp3 Gene Expression in Chronically Exposed Residents of the Urals Region. Medical Radiology and Radiation Safety. 2024;69(5):59–65. (In Russian). DOI:10.33266/1024-6177-2024-69-5-59-65
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out as part of the: «Study on the effect of chronic radiation exposure on the state of the human T-cell immune system».
Contribution. Kotikova AI ‒ laboratory research, statistical processing, article authoring; Nikiforov VS – laboratory research, statistical processing, article authoring; Blinova EA ‒ article authoring; Akleyev AV ‒ development of the research concept, scientific supervision, article authoring.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.