JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2025. Vol. 70. № 2
DOI:10.33266/1024-6177-2025-70-2-16-22
S.A. Abdullaev1, 2, 3, D.V. Fomina1, 3, V.O. Menukhov1, 2, M.V. Dushenko1,
A.V. Tochilenko4, T.P. Kalinin5, E.V. Evdokimovskii2
Changes in the Copy Number and Gene Expression of mtDNA
in Various Tissues of Mice Exposed to Local Brain Irradiation
1 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
2 Institute of Theoretical and Experimental Biophysics, Pushchino, Russia
3 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
4 National Research Nuclear University MEPhI, Moscow, Russia
5 N.I. Pirogov Russian National Research Medical University, Moscow, Russia
Contact person: S.A. Abdullaev, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To determine changes in the copy number and gene expression of mtDNA in various tissues of mice subjected to local irradiation of the brain.
Material and methods: Male Balb/c mice aged 2 months were used. Only the head of the mouse was exposed to X-ray irradiation at a dose of 5 Gy (power 2.5 Gy/min). After removal from the animals, the tissues were homogenized on ice, after which the homogenate mass was divided into two parts to isolate nucleic acids. Liquid blood was collected separately, after which nucleated blood cells were separated into granulocyte and monocyte fractions by differential centrifugation by Diacoll density gradient. The total number of mtDNA copies and gene expression were assessed using real-time PCR.
Results: It was shown that in nucleated blood cells, after irradiation, the relative number of transcripts of the mitochondrial gene ATP6 increases. In granulocytes, this effect is much more pronounced than in monocytes. At the same time, the amount of mitochondrial DNA in nucleated blood cells decreases relative to the control level by 2–3 times. In the brain exposed to irradiation, an increase in the relative amount of mtDNA transcripts by about 3 times is also observed, compared to the control. In organs not exposed to irradiation (heart, liver, spleen), the same effect is observed as in the brain, namely, an increase in the relative amount of mtDNA transcripts. The number of copies of mtDNA itself in brain cells, after a sharp increase a day after irradiation, sharply decreases and remains so until the end of the experiment, 30 days later. In liver and heart cells, the opposite process occurs, namely, a significant increase in the number of mtDNA copies, with a maximum at 14–21 days from the moment of irradiation.
Conclusion: Thus, the obtained results allow us to say that the observed changes are due to the occurrence of the “bystander effect” that arose after local irradiation of the brain with X-ray radiation at a dose of 5 Gy.
Keywords: mtDNA, bystander effect, oxidative stress, X-ray exposure, brain, mice
For citation: Abdullaev SA, Fomina DV, Menukhov VO, Dushenko MV, Tochilenko AV, Kalinin TP, Evdokimovskii EV. Changes in the Copy Number and Gene Expression of mtdna in Various Tissues of Mice Exposed to Local Brain Irradiation. Medical Radiology and Radiation Safety. 2025;70(2):16–22. (In Russian). DOI:10.33266/1024-6177-2025-70-2-16-22
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was supported by the Russian Science Foundation (project No. 24-24-00446).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.12.2024. Accepted for publication: 25.01.2025.