JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 4
DOI:10.33266/1024-6177-2025-70-4-10-15
D.V. Molodtsova1, 2, E.A. Kotenkova3, E.K. Polishchuk4, A.A. Osipov2,
D.V. Guryev1, A.K. Chigasova1, 2, 5, N.Yu. Vorobyeva1, 2, A.N. Osipov1, 2, 3
Sensitivity to Chemoradiation Effects of Human Non-Small Cell Lung Cancer Cells Surviving Fractionated Irradiation with a Total Dose of 20 Gy
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
3 Moscow Institute of Physics and Technology (National Research University), Moscow Region, Dolgoprudny, Russia
4 Experimental clinic and research laboratory for bioactive substances of animal origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia
5 N.M. Emanuel Institute for Biochemical Physics, Moscow, Russia
Contact person: D.V. Molodtsova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Objective: To obtain human non-small cell lung cancer (NSCLC) cells that survived and showed stable growth after fractionated exposure to X-rays at a total dose of 20 Gy and to evaluate their sensitivity to additional irradiation and cisplatin.
Material and methods: The NSCLC cell line A549 was used in the study, and it was irradiated in the fractionated mode (5 fractions of 4 Gy) to obtain a subline of surviving cells – A549IR. A549 and A549IR cells were subjected to testing exposure to X-rays or cisplatin. Then, proliferative activity, 2D migration capacity and the efficiency of DNA double-strand break (DSB) repair were analyzed using a quantitative assessment of residual foci of the γH2AX and 53BP1 proteins.
Results: The study yielded NSCLC cells that survived and showed stable growth after fractionated X-ray irradiation with a total dose of 20 Gy. The resulting A549IR cells had altered morphology, decreased proliferative activity, and increased migration capacity. Analysis of residual 53BP1 foci after test irradiation with a dose of 6 Gy indicates increased efficiency of repair of radiation-induced DNA DSBs. It was also found that A549IR cells are more resistant to cisplatin.
Conclusion: Overall, the study results show that combination CRT for the treatment of NSCLC should be prescribed with caution if studies based on the animal model support current conclusions. NSCLC cells that have survived IR exposure may acquire resistance to cisplatin. To select the appropriate therapy, it is important to assess both the existing radio- and chemo-resistance of tumor cells and their resistance to therapeutic effects that developed during treatment.
Keywords: NSCLC cells, Х-ray irradiation, сisplatin, γH2AX, 53BP1, residual foci, DNA double-strand breaks
For citation: Molodtsova DV, Kotenkova EA, Polishchuk EK, Osipov AA, Guryev DV, Chigasova AK, Vorobyeva NYu, Osipov AN. Sensitivity to Chemoradiation Effects of Human Non-Small Cell Lung Cancer Cells Surviving Fractionated Irradiation with a Total Dose of 20 Gy. Medical Radiology and Radiation Safety. 2025;70(4):10–15. (In Russian). DOI:10.33266/1024-6177-2025-70-4-10-15
References
1. Watanabe S-i., Nakagawa K., Suzuki K., Takamochi K., Ito H., Okami J., et al. Neoadjuvant and Adjuvant Therapy for Stage III Non-Small Cell Lung Cancer. Japanese Journal of Clinical Oncology. 2017;47;12:1112-8. doi: 10.1093/jjco/hyx147.
2. Hao W., Wu L., Cao L., Yu J., Ning L., Wang J., et al. Radioresistant Nasopharyngeal Carcinoma Cells Exhibited Decreased Cisplatin Sensitivity by Inducing SLC1A6 Expression. Frontiers in Pharmacology. 2021;12:629264. doi: 10.3389/fphar.2021.629264.
3. Gomez-Casal R., Epperly M.W., Wang H., Proia D.A., Greenberger J.S., Levina V. Radioresistant Human Lung Adenocarcinoma Cells that Survived Multiple Fractions of Ionizing Radiation are Sensitive to HSP90 Inhibition. Oncotarget. 2015;6;42:44306-22. doi: 10.18632/oncotarget.6248.
4. Wang Y., Huang J., Wu Q., Zhang J., Ma Z., Ma S., et al. Downregulation of Breast Cancer Resistance Protein by Long-Term Fractionated Radiotherapy Sensitizes Lung Adenocarcinoma to SN-38. Investigational New Drugs. 2021;39;2:458-68. doi: 10.1007/s10637-020-01003-3.
5. Payton C., Pang L.Y., Gray M., Argyle D.J. Exosomes Derived from Radioresistant Breast Cancer Cells Promote Therapeutic Resistance in Naïve Recipient Cells. Journal of Personalized Medicine. 2021;11;12. doi: 10.3390/jpm11121310.
6. Wang Y., Huang J., Wu Q., Zhang J., Ma Z., Zhu L., et al. Decitabine Sensitizes the Radioresistant Lung Adenocarcinoma to Pemetrexed Through Upregulation of Folate Receptor Alpha. Frontiers in Oncology. 2021;11:668798. doi: 10.3389/fonc.2021.668798.
7. Alhaddad L., Osipov A.N., Leonov S. The Molecular and Cellular Strategies of Glioblastoma and Non-Small-Cell Lung Cancer Cells Conferring Radioresistance. International Journal of Molecular Sciences. 2022;23;21:13577 doi: 10.3390/ijms232113577.
8. Molodtsova D., Guryev D.V., Osipov A.N. Composition of Conditioned Media from Radioresistant and Chemoresistant Cancer Cells Reveals miRNA and other Secretory Factors Implicated in the Development of Resistance. International Journal of Molecular Sciences. 2023;24;22:16498. doi: 10.3390/ijms242216498.
9. Babayan N., Grigoryan B., Khondkaryan L., Tadevosyan G., Sarkisyan N., Grigoryan R., et al. Laser-Driven Ultrashort Pulsed Electron Beam Radiation at Doses of 0.5 and 1.0 Gy Induces Apoptosis in Human Fibroblasts. International Journal of Molecular Sciences. 2019;20;20:51-40 doi: 10.3390/ijms20205140.
10. Shibata A., Jeggo P.A. Roles for 53BP1 in the Repair of Radiation-Induced DNA Double Strand Breaks. DNA Repair. 2020;93:102915. doi: 10.1016/j.dnarep.2020.102915.
11. Osipov A.N., Pustovalova M., Grekhova A., Eremin P., Vorobyova N., Pulin A., et al. Low Doses of X-Rays Induce Prolonged and ATM-Independent Persistence of γH2AX Foci in Human Gingival Mesenchymal Stem Cells. Oncotarget. 2015;6;29:27275-87. doi: 10.18632/oncotarget.4739.
12. Osipov A., Chigasova A., Yashkina E., Ignatov M., Vorobyeva N., Zyuzikov N., et al. Early and Late Effects of Low-Dose X-ray Exposure in Human Fibroblasts: DNA Repair Foci, Proliferation, Autophagy, and Senescence. International Journal of Molecular Sciences. 2024;25;15:8253 doi: 10.3390/ijms25158253.
13. Chigasova A.K., Pustovalova M.V., Osipov A.A., Korneva S.A., Eremin P.S., Yashkina E.I., et al. Post-Radiation Changes in The Number of Phosphorylated H2ax and Atm Protein Foci in Low Dose X-Ray Irradiated Human Mesenchymal Stem Cells. Medical Radiology and Radiation Safety. 2024;69;1:15-9. doi: 10.33266/1024-6177-2024-69-1-15-19.
14. Osipov A., Chigasova A., Belov O., Yashkina E., Ignatov M., Fedotov Y., et al. Dose Threshold for Residual γH2AX, 53BP1, pATM and p-p53 (Ser-15) Foci in X-Ray Irradiated Human Fibroblasts. International Journal of Radiation Biology. 2025;101;3:1-10. doi: 10.1080/09553002.2024.2445581.
15. Osipov A., Chigasova A., Yashkina E., Ignatov M., Fedotov Y., Molodtsova D., et al. Residual Foci of DNA Damage Response Proteins in Relation to Cellular Senescence and Autophagy in X-Ray Irradiated Fibroblasts. Cells. 2023;12;8:1209 doi: 10.3390/cells12081209.
16. Djuzenova C.S., Zimmermann M., Katzer A., Fiedler V., Distel L.V., Gasser M., et al. A Prospective Study on Histone γ-H2AX and 53BP1 Foci Expression in Rectal Carcinoma Patients: Correlation with Radiation Therapy-Induced Outcome. BMC Cancer. 2015;15;1:856. doi: 10.1186/s12885-015-1890-9.
17. Katsube T., Mori M., Tsuji H., Shiomi T., Wang B., Liu Q., et al. Most Hydrogen Peroxide-Induced Histone H2AX Phosphorylation is Mediated by ATR and is not Dependent on DNA Double-Strand Breaks. Journal of Biochemistry. 2014;156;2:85-95. doi: 10.1093/jb/mvu021.
18. Arcangeli S., Greco C. Hypofractionated Radiotherapy for Organ-Confined Prostate Cancer: is Less More? Nature Reviews Urology. 2016;13;7:400-8. doi: 10.1038/nrurol.2016.106.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The research was carried out with the support of the State Research Assignment cipher «Signal» (registration number in the EGISU R&D system: 123011200048-4).
Contribution. Writing: D.V. Molodtsova, A.N. Osipov; Experimental planning: D.V. Molodtsova, N.Yu. Vorobyeva, A.N. Osipov, D.V. Guryev; Experimental work: D.V. Molodtsova, N.Yu. Vorobyeva, E.A. Kotenkova, E.K. Polishchuk, A.A. Osipov, A.K. Chigasova; Vizualization: A.N. Osipov.
Article received: 20.03.2025. Accepted for publication: 25.04.2025.