SCIENTIFIC AND INFORMATION-ANALYTICAL MAGAZINE

ISSN 1024-6177 (Print), ISSN 2618-9615 (Online)

JOURNAL DESCRIPTION

The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.

Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.

Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.

The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.

Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.

The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.

Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.

The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.

Medical Radiology and Radiation Safety. 2018. Vol. 63. No. 1. P. 28-34

RADIATION BIOLOGY

DOI: 10.12737/article_5a855c9d5b1211.49546901

3H-Thymidine Influence on DNA Double Strand Breaks Induction in Cultured Human Mesenchymal Stem Cells

N.Yu. Vorobyeva, V.V. Uyba, O.A. Kochetkov, T.A. Astrelina, M.V. Pustovalova, A.K. Grekhova, T.M. Blokhina, E.I. Yashkina, D.I. Kabanov, V.A. Nikitina, Yu.B. Suchkova, I.V. Kobzeva, A.N. Osipov

A.I. Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

N.Yu. Vorobyeva – Senior Researcher, PhD Biol.; V.V. Uyba – Head of the Federal Medical Biological Agency of Russia, Dr. Sc. Med., Prof.; O.A. Kochetkov – Head of Lab., PhD Tech.; T.A. Astrelina – Head of Center for Biomedical Technologies, Dr. Sc. Med.; M.V. Pustovalova – Research Fellow; A.K. Grekhova – Junior Researcher; T.M. Blokhina – Research Fellow; E.I. Yashkina – Junior Researcher; D.I. Kabanov – Engineer; V.A. Nikitina – Leading Researcher, PhD Med.; Yu.B. Suchkova – Senior Researcher, PhD Med.; I.V. Kobzeva – Senior Researcher, PhD Med.; A.N. Osipov – Head of Dep., Dr. Biol. Sc., Prof.

Abstract

Purpose: To estimate the impact of 3H-thymidine on DNA double strand breaks (DSBs) induction in cultured human mesenchymal stem cells (MSC).

Material and methods: Isolation and cultivation of human bone marrow MSC was carried out according to a standard procedure. A sterile solution of 3H-thymidine with different specific radioactivity was added to the cell culture and incubated under the conditions of the CO2 incubator for 24 hours. The specific radioactivity of 3H-thymidine in the incubation medium was 50–1600 kBq/ml. To evaluate quantitatively the DSBs, an immunocytochemical analysis of the DSB marker – phosphorylated histone (γH2AX) foci was used. Additionally, the proportion of dividing cells was estimated using an immunocytochemical analysis of the cell proliferation marker, the Ki67 protein.

Results: It was shown that 24 h incubation of human MSC in a culture medium results in a dose-dependent increase in γH2AX foci. There is a linear increase in the foci γH2AX in the range of 50–400 kBq/ml, after which the relative quantitative yield of foci per unit of specific radioactivity begins to decrease. In general, the dose-effect relationship is approximated by the quadratic function y = 3.13 + 50.80x – 12.38x2 (R2 = 0.99), where y is the number of foci γH2AX in the cell nucleus, and x is the specific radioactivity in 1000 kBq/ml. It was found that incubation of human MSC in a culture medium containing 800 and 1600 kBq/ml of 3H-thymidine resulted in a statistically significant decrease in the cells proliferative activity compared to the control of ~1.25 and 1.41 respectively. The peculiar biological limitation of tritium accumulation in the cell nucleus explains well the nonlinear character of the dependence of the formation of DSBs on the specific radioactivity of 3H-thymidine in the culture medium observed in our study.

Conclusion: Quantitative analysis of γH2AX foci has proved to be a highly reproducible and highly sensitive method for evaluating the induction of DSBs in living cells under the action of 3H-thymidine. An analysis of the foci of γH2AX will be useful for accurate estimating the quantitative yield of DBS in living cells per dose of 3H-thymidine β-radiation. To do this, it is necessary to make a correct calculation of the doses received by the cells taking into account the microdistribution of 3H-thymidine in the cell volume and its accumulation in the DNA of living cells.

Key words: 3H-thymidine, DNA double strand breaks, γH2AX foci, mesenchymal stem cells

REFERENCES

  1. Kotzer T, Trivedi A. Dosimetric implications of atmospheric dispersal of tritium near a heavy-water research reactor facility. Radiat. Prot. Dosim. 2001;93:61-6.
  2. Okada S, Momoshima N. Overview of tritium. characteristics, sources, and problems. Health Phys. 1993;65:595-609.
  3. Alloni D, Cutaia C, Mariotti L, et al. Modeling dose deposition and DNA damage due to low-energy beta(-) emitters. Radiat. Res. 2014;182:322-30.
  4. Melintescu A, Galeriu D. Uncertainty of current understanding regarding OBT formation in plants. J. Environ. Radioact. 2017;167:134-49.
  5. Dingwall S, Mills CE, Phan N, et al. Human health and the biological effects of tritium in drinking water. Prudent policy through science  addressing the ODWAC new recommendation. Dose Response. 2011;9:6-31.
  6. Umata T. Estimation of biological effects of tritium. J. UOEH. 2017;39:25-33.
  7. Little MP, Lambert BE. Systematic review of experimental studies on the relative biological effectiveness of tritium. Radiat. Environ. Biophys. 2008;47:71-93.
  8. Task Group on Radiation Quality Effects in Radiological Protection CoREICoRP. Relative biological effectiveness (RBE), quality factor (Q), and radiation weighting factor (w(R)). A report of the International Commission on Radiological Protection. Ann ICRP. 2003;33:1-117.
  9. Harrison J. Doses and risks from tritiated water and environmental organically bound tritium. J. Radiol. Prot. 2009;29:335-49.
  10. Balonov MI, Muksinova KN, Mushkacheva GS. Tritium radiobiological effects in mammals. review of experiments of the last decade in Russia. Health Phys. 1993;65:713-26.
  11. Balonov MI, Chipiga LA. [Estimation of the dose from the intake of tritium oxide in the human body. The role of inclusion of tritium in the organic matter of tissues]. Radiats. Gigiena [Radiation Hygiene]. 2016;9(4):16-25. DOI: 10.21514/1998-426X-2016-9-4-16-25. Russian.
  12. Kochetkov OA, Monastyrskaya SG, Kabanov DI. [Problems of rationing technogenic tritium]. Saratov Journal of Medical Scientific Research. 2013;9(4):815-19. (English abstracts. Available from: https://cyberleninka.ru/article/n/problemy-normirovaniya-tehnogennogo-tritiya). Russian.
  13. Korzeneva IB, Kostuyk SV, Ershova LS, et al. Human circulating plasma DNA significantly decreases while lymphocyte DNA damage increases under chronic occupational exposure to low-dose gamma-neutron and tritium β-radiation. Mutation Res./Fundamental and Molecular Mechanisms of Mutagenesis. 2015;779:1-15.
  14. Osipov AN, Grekhova A, Pustovalova M, et al. Activation of homologous recombination DNA repair in human skin fibroblasts continuously exposed to X-ray radiation. Oncotarget. 2015;6:26876-85.
  15. Osipov AN, Pustovalova M, Grekhova A, et al. Low doses of X-rays induce prolonged and ATM-independent persistence of gammaH2AX foci in human gingival mesenchymal stem cells. Oncotarget. 2015;6:27275-87.
  16. Kotenko KV, Bushmanov AY, Ozerov IV, et al. Changes in the number of double-strand DNA breaks in Chinese hamster V79 cells exposed to gamma-radiation with different dose rates. Int. J. Mol. Sci. 2013;14:13719-26.
  17. Halazonetis TD, Gorgoulis VG, Bartek J. An oncogene-induced DNA damage model for cancer development. Science. 2008;319:1352-55.
  18. Chen J. Estimated yield of double-strand breaks from internal exposure to tritium. Radiat. Environ. Biophys. 2012;51:295-302.
  19. Moiseenko VV, Waker AJ, Hamm RN, Prestwich WV. Calculation of radiation-induced DNA damage from photons and tritium beta-particles. Part II. Tritium RBE and damage complexity. Radiat. Environ. Biophys. 2001;40:33-8.
  20. Vignard J, Mirey G, Salles B. Ionizing-radiation induced DNA double-strand breaks. A direct and indirect lighting up. Radiother. Oncol. 2013;108(3):362-9.
  21. Sharma A, Singh K, Almasan A. Histone H2AX phosphorylation. a marker for DNA damage. Methods Mol. Biol. 2012;920:613-26.
  22. Tsvetkova A, Ozerov IV, Pustovalova M, et al. γH2AX, 53BP1 and Rad51 protein foci changes in mesenchymal stem cells during prolonged X-ray irradiation. Oncotarget. 2017;8(38):64317-29.
  23. Grekhova AK, Eremin PS, Osipov AN, et al. [Delayed processes of γH2AX foci formation and degradation in human skin fibroblasts irradiated with x-rays in low doses]. Radiats. Biol. Radioekologia (‘Radiation biology. Radioecology’). 2015;55(4):395-401. (English abstracts. PubMed PMID: 26601539). Russian.
  24. Pustovalova M, Grekhova A, Astrelina Ь et al. Accumulation of spontaneous γH2AX foci in long-term cultured mesenchymal stromal cells. Aging (Albany NY). 2016;8(12):3498-506.
  25. Pustovalova M, Astrelina с, Grekhova A, et al. Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells. Aging (Albany NY). 2017;9(11):2397-410.
  26. Paull TT, Rogakou EP, Yamazaki V, et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr. Biol. 2000;10:886-95.
  27. Lobrich M, Shibata A, Beucher A, et al. GammaH2AX foci analysis for monitoring DNA double-strand break repair. strengths, limitations and optimization. Cell Cycle. 2010;9:662-9.
  28. Gonen RGU, Alfassi ZB, Priel E. Production of DNA double strand breaks in human cells due to acute exposure to tritiated water (HTO). Conference of the Nuclear Societies in Israel; 2014 Feb 11-13. Dead Sea (Israel). p. 69-73.
  29. Saintigny Y, Roche S, Meynard D, Lopez BS. Homologous recombination is involved in the repair response of mammalian cells to low doses of tritium. Radiat. Res. 2008;170:172-83.
  30. Ozerov IV, Osipov AN. [Kinetic model of repair of DNA double-strand breaks in primary human fibroblasts under the action of rare-ionizing radiation with different dose rates]. Kompiuternye issledovania i modelirovanie [Computer Studies and Modeling]. 2015;7(1):159-76. (English abstracts. Available from: http://crm-en.ics.org.ru/journal/article/2237/). Russian.
  31. Ozerov IV, Eremin PS, Osipov AN, et al. [Features of changes in the number of foci of proteins γH2AX and Rad51 in human fibroblasts subjected to prolonged exposure to low-intensity X-rays]. Saratov Scientific-Medical Journal. 2014;10(4):739-43. (English abstracts. Available from: https://cyberleninka.ru/article/n/osobennosti-izmeneniya-chisla-fokusov-belkov-un2ah-i-rad51-v-fibroblastah-kozhi-cheloveka-podvergavshihsya-prolongirovannomu). Russian.
  32. Ozerov IV, Bushmanov AYu, Anchishkina NA, et al. [Induction and repair of DNA double-strand breaks in V79 cells under long-term exposure to low-intensity γ-radiation]. Saratov Scientific-Medical Journal. 2013;9(4):787-91.(English abstracts. Available from: https://cyberleninka.ru/article/n/induktsiya-i-reparatsiya-dvunitevyh-razryvov-dnk-v-kletkah-linii-v79-pri-dlitelnom-vozdeystvii-nizkointensivnogo-u-izlucheniya). Russian.
  33. Duque A, Rakic P. Different effects of BrdU and 3H-Thymidine incorporation into DNA on cell proliferation, position and fate. J. Neurosci. 2011;31(42):15205-17.
  34. Hoy CA, Lewis ED, Schimke RT. Perturbation of DNA replication and cell cycle progression by commonly used [3H]thymidine labeling protocols. Mol. Cell Biol. 1990 Apr;10(4):1584-92.
  35. Hu VW, Black GE, Torres-Duarte A, Abramson FP. 3H-thymidine is a defective tool with which to measure rates of DNA synthesis. FASEB J. 2002;16(11):1456-7.
  36. Jurikova M, Danihel L, Polak S. Varga I. Ki67, pcna, and mcm proteins. Markers of proliferation in the diagnosis of breast cancer. Acta Histochemica 2016;118:544-52.

For citation: Vorobyeva NYu, Uyba VV, Kochetkov OA, Astrelina TA, Pustovalova MV, Grekhova AK, Blokhina TM, Yashkina EI, Kabanov DI, Nikitina VA, Suchkova YuB, Kobzeva IV, Osipov AN. 3H-Thymidine Influence on DNA Double Strand Breaks Induction in Cultured Human Mesenchymal Stem Cells. Medical Radiology and Radiation Safety. 2018;63(1):28-34. DOI: 10.12737/article_5a855c9d5b1211.49546901. Russian.

PDF (RUS) Full-text article (in Russian)

Contact Information

 

46, Zhivopisnaya st., 123098, Moscow, Russia Phone: +7 (499) 190-95-51. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Journal location

Attendance

2943154
Today
Yesterday
This week
Last week
This month
Last month
For all time
4251
2306
20363
33458
41450
113593
2943154
Forecast today
4512

Your IP:216.73.216.100
Visitor Counter

© Журнал "Медицинская радиология и радиационная безопасность" 2020г.

Яндекс.Метрика

Наверх