JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
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Medical Radiology and Radiation Safety. 2018. Vol. 63. No. 4. P. 33-39
RADIATION MEDICINE
DOI: 10.12737/article_5b83b6185814e7.31842273
Transcriptional Activity of TP53 and MDM2 in Chronically Exposed People at Later Time Points
V.S. Nikiforov1,2 , A.V. Akleyev1,2
1. Urals Research Center for Radiation Medicine, Chelyabinsk, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Chelyabinsk State University, Chelyabinsk, Russia
V.S. Nikiforov - Postgraduate Student, Junior Researcher; A.V. Akleyev - Dr. Sci. Med., Prof., Director, Urals Research Center for Radiation Medicine
Abstract
Purpose: To study the levels of transcriptional activity of ТР53 and MDM2 genes in the residents of the Techa riverside villages chronically exposed at a wide dose range.
Material and methods: transcriptional activity of ТР53 and MDM2 genes was assessed in 95 persons. The main study group included 80 people exposed to combined external and internal radiation (peripheral blood samples were taken 60-70 years after the beginning of chronic radiation exposure), mean accumulated dose to red bone marrow was 0.86 ± 0.08 Gy (doses varied in the range 0.1-3.65 Gy). The control group consisted of 15 people living in similar socio-economic conditions in the Southern Urals; the accumulated doses to red bone marrow did not exceed 0.07 Gy. Gene transcription activity profile was studied with real-time PCR assay. The data were analyzed using a comparative CT method with normalization to the “housekeeping” gene transcription in each sample. Statistical analysis was performed using the software PAST.
Results and сonclusion: In the course of the analysis we did not receive statistically significant differences between the study groups, but there was a tendency to a decrease in gene transcription in the group of exposed persons. The correlation analysis showed a weak negative dependence for TP53 and MDM2 genes, and this dependence was characterized not only by the accumulated dose value but was also associated with the age of the individuals under study. A tendency to a decrease in the transcription activity of the genes under study was noted when studying the effect of the dose. Statistically significant differences were shown for MDM2 gene in the group of individuals whose accumulated doses exceeded 2 Gy (p = 0.044). The analysis of age-peculiarities on gene transcription revealed a statistically significant decrease in TP53 gene transcription with increasing age of patients (p = 0.02). Non-radiation factors including smoking were also studied. The levels of gene transcription were compared between men and women of 2 main ethnicities (Bashkirs/Tartars and Slavs). Results of the study showed that neither sex nor ethnicity had any effect on the levels of TP53 and MDM2 gene transcription in the study groups. The effect of smoking on the activity of the genes under study was negligible.
Key words: chronic irradiation, gene expression, TP53, MDM2, low doses, radiobiological response
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For citation: Nikiforov VS, Akleyev AV. Transcriptional Activity of TP53 and MDM2 in Chronically Exposed People at Later Time Points. Medical Radiology and Radiation Safety. 2018;63(4):33-9. Russian. DOI: 10.12737/article_5b83b6185814e7.31842273
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