Medical Radiology and Radiation Safety. 2016. Vol. 61. No. 4. P. 35-42

RADIATION SAFETY

V.Yu. Nugis, A.Yu. Bushmanov, H.E. Zapadinskaya, M.G. Kozlova, O.A. Tyhonova

Cytogenetic Studies 28-29 Years After the Accident at the Chernobyl NPP

A.I. Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

ABSTRACT

Purpose: Cytogenetic examination of liquidators who worked in different years and residents of different contaminated areas nearly 30 years after the accident at the Chernobyl nuclear power plant.

Material and methods: The cytogenetic studies were carried out with the use of peripheral blood lymphocyte cultures. The majority of individuals were examined with the help of FISH- and classical methods of staining chromosomes in parallel.

Results: In general the frequencies of FISH-detected translocations were above background levels in 15 (out of 58 people): 12 out of 41 liquidators and 3 out of 17 residents of contaminated territories. The single highly aberrant cells contained mostly unstable chromosome aberrations were detected by FISH-analysis in 9 examined individuals. The presence or absence of such cells was not associated with the availability of an estimated dose cytogenetically. The analysis of the data obtained the classical method showed that the overall frequency of chromosome rearrangements and the frequency of chromosome aberrations - indicators of radiation exposure - did not exceed the background values of the examined persons individually.

Conclusion: The obtained cytogenetic data indicate the possibility of cytogenetic dose evaluation in almost 30 years after the radiation accident with the help of FISH-method. The detection of single highly aberrant cells in peripheral blood lymphocyte cultures is characteristic for this contingent that apparently related to the action of alpha-emitting radionuclides.

Key words: chromosome aberrations, peripheral blood, lymphocytes culture, accidental irradiation, the accident at the Chernobyl NPP

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For citation: Nugis VYu, Bushmanov AYu, Zapadinskaya HE, Kozlova MG, Tyhonova OA. Cytogenetic Studies 28-29 Years after the Accident at the Chernobyl NPP. Medical Radiology and Radiation Safety. 2016;61(4):35-42. Russian.

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