Medical Radiology and Radiation Safety. 2014. Vol. 59. No. 3. P. 12-15
RADIATION BIOLOGY
A.A. Ivanov, G.A. Shal’nova, V.N. Mal’tsev, A.M. Ulanova, N.M. Stavrakova, T.M. Bulinina, V.G. Scachcova
Bacteriuria in Experimental Radiation Disease
Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: The investigation of urine microflora of irradiated mice using paper disks.
Material and methods: The method of paper discs was used for study of bacteriuria in mice (CBA×C57Bl)F1 after total body γ-irradiation at 5.0 (LD10/30), 6.5 (LD50–80/30), 7.0 (LD90/30) and 10,0 Gy (LD100/8). The paper disks being impregnated with one drop of urine, were placed on the Endo media surface and incubated in thermostat (37 °C) during 24 hours.
Results: It was shown that bacteriuria appeared in latent period with maximum in the period of main manifestations of acute radiation disease and disappeared at period of recovery. Its expression was proportional to the grade of acute radiation disease.
Conclusion: The method of paper disks can be used in experimental practice for study of infectious complication of radiation disease in mice.
Key words: acute radiation disease, paper discs, Endo media, γ-radiation, urina, enterobacter, enterococcus