Medical Radiology and Radiation Safety. 2012. Vol. 57. No. 5. P. 5-10

RADIATION BIOLOGY

 K.V. Kotenko¹, I.K. Belyaev¹, Yu.P. Buzulukov², А.Yu. Bushmanov¹, V.F. Demin², I.V. Gmoshinski³, E.S. Zhorova¹, V.S. Kalistratova¹, N.S. Marchenkov², P.G. Nisimov¹, R.V. Raspopov³, V.Yu. Soloviev¹

Experimental Research of Zinc Oxide-labeled Nanoparticles Biokinetics in Rats’ Organism after Single Oral Administration by Labeled Atoms Technology

1. Burnasyan Federal Medical Biophysical Center (FMBC) of Federal Medical Biological Agency, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Russian Research Center Kurchatov Institute, Moscow, Russia; 3. Institute of Nutrition of RAMS, Moscow, Russia

Abstract

Purpose: To make quantitative assessment of zinc oxide-labeled nanoparticles’ biokinetics in laboratory animals’ (rats’) organism after single oral administration.

Material and methods: Suspension of zinc oxide nanoparticles with 30 nmmedian diameter was used as source material. Before the injection nanoparticles were irradiated to thermal neutron beam, so that part of the atoms was activated with formation of radioactive nucleus of ⁶⁵Zn with half-life of 234.8 days. Four groups of animals were used in the experiment (3 male Wistar rats with average body weight of ~ 170 g in each group). According to number of animals used in the experiment, the suspension of nanoparticles was divided into 12 parts of 0.5 mL each. Animals were sacrificed at hours 4, 24, 72 and 120 after administration; then organs of the sacrificed animals including brain, heart, lung, liver, spleen, pancreas, kidneys, testicle, also remained carcass, blood, excrement and urine were analyzed. Changes of organs’ activity were recorded on low-background gamma-ray spectrometer.

Results: Biokinetic parameters of Vistar rat-males are evaluated after single oral administration of zinc oxide nanoparticles. Maximum content of nanoparticles was observed in 24-72 hours after oral administration. ⁶⁵Zn mass distribution in rats’ organs at maximum point decreases according to the following sequence: liver®kidneys®spleen ®pancreas®heart®blood®brain.

Conclusion: 1. Technology of producing new type of product - labeled nanoparticles by means of irradiating them with thermal neutrons of nuclear reactor is elaborated. 2. It is proved that there is a possibility to do research of nanoparticles’ biokinetics in the laboratory animal organisms using labeled atoms technology. 3. ⁶⁵Zn activity records in the rats’ brain (near 0.06 % of administered activity) proves that nanoparticles can mount blood brain barrier.

Key words: nanoparticles, zinc oxide, radioactive label, experiment, rats, biokinetics, blood brain barrier

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For citation: Kotenko KV, Belyaev IK, Buzulukov YuP, Bushmanov АYu, Demin VF, Gmoshinski IV, Zhorova ES, Kalistratova VS, Marchenkov NS, Nisimov PG, Raspopov RV, Soloviev VYu. Experimental Research of Zinc Oxide-labeled Nanoparticles Biokinetics in Rats’ Organism after Single Oral dministration by Labeled Atoms Technology. Medical Radiology and Radiation Safety. 2012;57(5):5-10.

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